GENE CONSTRUCT-BASED SERINE PROTEASE OF Bacillus pumilus AS A FEED ADDITIVE FOR POULTRY FARMING

The objective of the study was to determine the effect of adding protease obtained from the Bacillus pumilus 3-19 strain to the diet of Hubboard broiler chickens on the growth indicators of poultry and the composition of the cecum microbiota of broiler chickens. As a result of metagenomic analysis of 16S rRNA genes in the contents of the cecum of chickens, it was shown that the control and experimental groups formed two separate clusters, which indicates differences in microbial communities in these two groups. Analysis of alpha diversity showed an increase in species richness in the group of chickens that received protease as a feed additive. On day 35, both groups were dominated by bacterium of Firmicutes (21.13 — 49.01%) and Bacteroidetes (37.68 — 67.72%) groups. It was shown that in the group of chickens receiving protease as a feed additive, the average daily gain in live weight was higher than in the control group by 0.5 kg (P < 0.05). The introduction of protease resulted in an increased F/B ratio, which was positively correlated with chicken body weight. Thus, the addition of B. pumilus 3-19 protease to broiler feed leads to improved feed intake and digestibility, which is of great importance for reducing the cost of the final product. The use of protease regulates the cecum microbiota of broiler chickens and increases microbial diversity on 35 day of chicken growth.

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“…Subtilisin-like proteinase was isolated and purified using the B. pumilus 3-19 strain, as described earlier [13].…”

Section: Methods Of Research 21 Creation Of the Enzymementioning

confidence: 99%

Effect of Bacillus pumilus 3-19 protease on growth parameters and gut microbiome of broiler chickens

et al. 2020

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“…Subtilisin-like proteinase was isolated from the B. pumilus 7 p strain and purified as previously described by (Koryagina et al, 2018). B. pumilus 7 p is a natural soil isolate, which shows an increased level of subtilisinlike proteinase secretion.…”

Section: Extraction and Purification Of Bacterial Enzymesmentioning

confidence: 99%

“…At a bile concentration of 5%, the residual activity of the enzyme was 60%. Its activity was not suppressed by natural inhibitors, such as trypsin inhibitor, which allows it to function in the gastrointestinal tract of chickens (Koryagina et al, 2018). Grounding on the abovementioned properties, the obtained enzymes were concluded to be prospective feed supplements for poultry.…”

mentioning

confidence: 95%

The Effect of Bacterial Enzyme-Based Feed Additives on the Productivity, Digestibility and Assimilation of Nutrients in Young Laying Hens

Yur’evich¹,

Леонидовна²,

Olegovna³

et al. 2020

OnLine Journal of Biological Sciences

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GENE CONSTRUCT-BASED SERINE PROTEASE OF Bacillus pumilus AS A FEED ADDITIVE FOR POULTRY FARMING

Cited by 2 publications

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Effect of Bacillus pumilus 3-19 protease on growth parameters and gut microbiome of broiler chickens

Effect of Bacillus pumilus 3-19 protease on growth parameters and gut microbiome of broiler chickens

The Effect of Bacterial Enzyme-Based Feed Additives on the Productivity, Digestibility and Assimilation of Nutrients in Young Laying Hens

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