Gene clusters encoding the cytotoxic necrotizing factor type 1, Prs-fimbriae and α-hemolysin form the pathogenicity island II of the uropathogenic Escherichia coli strain J96

Blum, G

doi:10.1016/0378-1097(95)00009-t

Cited by 97 publications

(148 citation statements)

References 11 publications

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“…In a study by Sabate et al, PAI IV536 was found most frequently in both commensal and UPEC isolates from patients with pyelonephritis and urinary sepsis [14]. In most studies worldwide, the presence of PAI IV536 in the Enterobacteriace family is documented, and this marker is known as broad-hostrange PAI or high-pathogenicity island (HPI) [11,18,19]. Our results also showed that one UPEC isolate from patients with an implanted catheter and four UPEC isolates from patients without urinary catheters suffering from sepsis carried only PAI IV536 as a single PAI marker.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Firoozeh

Soleimani-Moorchekhorti

Zibaei

2017

J Infect Dev Ctries

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Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs) .The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536 were not detected in the UPEC strains. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.

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Section: Discussionmentioning

confidence: 99%

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Firoozeh

Soleimani-Moorchekhorti

Zibaei

2017

J Infect Dev Ctries

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“…Pathogenicity-associated islands are common among ExPEC (Blum et al, 1995;Chen et al, 2006;Lloyd et al, 2007) and have some characteristic features, including: (1) disparate G+C content from the core genome; (2) association with pathogenic organisms, but not commensal counterparts; (3) contain virulence-associated genes; (4) insertion sites or flanking direct repeats; (5) a high frequency of insertion at tRNA sites and (6) often mobility (pseudo)genes (Dobrindt et al, 2004;Gal-Mor & Finlay, 2006;Hacker & Kaper, 2000). PAI-X was associated with E. coli isolated from patients experiencing a variety of clinical syndromes, including asymptomatic bacteriuria, cystitis, (Hannan et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli

et al. 2013

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Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173583.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120587.5 %) than upper urinary tract origin (39/53574 %; P,0.05) or commensal isolates (28/78536 %; P¡0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.

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“…The pap/prs gene clusters coding for P®mbriae and the cnf1 gene are actually associated with a hly operon coding for the ahemolysin, forming the so-called pathogenicity island 5 or Pai 5 (Blum et al, 1995;De Rycke et al, 1999;Dozois and Curtiss III, 1999).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970

Bost

Bâbe

Jacquemin

et al. 2001

Veterinary Microbiology

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A total of 434 Escherichia coli isolated from septicemic calves between 1958 and 1965 and 430 E. coli isolated from diarrheic calves between 1967 and 1970 were studied by colony hybridisation and PCR assays for the presence of the cnf1-and the cnf2-like genes. They were also studied for the presence of genes coding for putative virulence factors associated with the CNF toxins including F17-, Pap-and Sfa-®mbrial adhesins and the recently described CDT-III toxin and AfaVIII-a®mbrial adhesin. Thirty (7%) of the 434 septicemic strains were positive for CNF by colony hybridisation. Twenty-six were con®rmed as necrotoxigenic E. coli type 2 (NTEC2) and four as NTEC1 by PCR. Thirty-®ve (8%) of the 430 diarrheic strains were positive for CNF by colony hybridisation. Five of them were studied by PCR and con®rmed as NTEC1. The 26 septicemic NTEC2 strains and 20 of the 35 diarrheic NTEC including three of the ®ve NTEC1 were positive for CDT-III. All adhesins studied were present in NTEC as well as in non-NTEC. NTEC1 were mainly Pap-, Sfa-and/or Afa8-positive, whereas NTEC2 were mainly F17-and/or Afa8-positive. This study shows that necrotoxigenic E. coli with their associated adhesins and toxins were present in calves as early as 1958, but their prevalence seems to have increased since that time. #

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Gene clusters encoding the cytotoxic necrotizing factor type 1, Prs-fimbriae and α-hemolysin form the pathogenicity island II of the uropathogenic Escherichia coli strain J96

Cited by 97 publications

References 11 publications

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli

Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970

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