Gene Cluster Conferring Streptomycin, Sulfonamide, and Tetracycline Resistance in Escherichia coli O157:H7 Phage Types 23, 45, and 67

Abstract

Multidrug resistance to streptomycin, sulfonamide, and tetracycline (AMR-SSuT) was identified in 156 of 171 isolates of Escherichia coli O157:H7 of phage types 23, 45, and 67. In 154 AMR-SSuT isolates, resistance was encoded by strA, strB, sul2, and tet(B), which in 59 of 63 tested isolates were found clustered together on the chromosome within the cdiA locus.

Bacteriophages Contribute to the Spread of Antibiotic Resistance Genes among Foodborne Pathogens of the Enterobacteriaceae Family – A Review

“…In a Canadian study, Ziebell et al (2011) characterized 187 isolates of STEC O157:H7 and observed that 45 of these possessed resistance to streptomycin, sulfonamide, and tetracycline. Of the 45 isolates, 43 belonged to three phage types: PT23, PT45, and PT67.…”

No evidence for a bovine mastitisEscherichia colipathotype

“…It partly contains the biofilmassociated polysaccharide synthesis pga locus. The resistance-related genes of GI4 are located on the AMR-SSuT (antimicrobial multidrug resistance to streptomycin, sulfonamide, and tetracycline) island, which is prevalent in E. coli from the bovine habitat [33,34]. In summary, the MGEs of MAEC strains 1303 and ECC-1470 do not carry many known virulence-associated genes, which may entail an advantage to mastitis pathogens.…”

“…It partly contains the biofilmassociated polysaccharide synthesis pga locus plus flanking genes identified as mastitisassociated by Goldstone et al [35]. The resistance-related genes of GI4 are located on the AMR-SSuT island (antimicrobial multidrug resistance to streptomycin, sulfonamide, and tetracycline), which is prevalent in E. coli from the bovine habitat [78,79]. The encoded resistance genes are strAB, sul2, and tetDCBR.…”

“…The encoded resistance genes are strAB, sul2, and tetDCBR. A comparison of the corresponding genomic region of E. coli 1303 with two publicly available AMR-SSuT island sequences from E. coli strain SSuT-25 [78] and E. coli O157:H7 strain EC20020119 [79] is shown in Additional file 4: Figure S5. Transposon Tn10, also present on the resistance plasmid R100, is an integral part of the AMR-SSuT island and comprises the tetDCBR genes.…”

“…The same workflow was done for the antimicrobial multidrug resistance element of 1303 (AMR-SSuT in GI4) in comparison to the E. coli SSuT-25 AMR-SSuT element [78] (accession number: EF646764), the E. coli O157:H7 EC20020119 AMR-SSuT region (accession number: HQ018801) [79], and transposon Tn10 of Shigella flexneri 2b plasmid R100 (accession number: AP000342).…”