Gene cluster analysis for the biosynthesis of elgicins, novel lantibiotics produced by paenibacillus elgii B69

Abstract

BackgroundThe recent increase in bacterial resistance to antibiotics has promoted the exploration of novel antibacterial materials. As a result, many researchers are undertaking work to identify new lantibiotics because of their potent antimicrobial activities. The objective of this study was to provide details of a lantibiotic-like gene cluster in Paenibacillus elgii B69 and to produce the antibacterial substances coded by this gene cluster based on culture screening.ResultsAnalysis of the P. elgii B69 genome sequence revealed the presence of a lantibiotic-like gene cluster composed of five open reading frames (elgT1, elgC, elgT2, elgB, and elgA). Screening of culture extracts for active substances possessing the predicted properties of the encoded product led to the isolation of four novel peptides (elgicins AI, AII, B, and C) with a broad inhibitory spectrum. The molecular weights of these peptides were 4536, 4593, 4706, and 4820 Da, respectively. The N-terminal sequence of elgicin B was Leu-Gly-Asp-Tyr, which corresponded to the partial sequence of the peptide ElgA encoded by elgA. Edman degradation suggested that the product elgicin B is derived from ElgA. By correlating the results of electrospray ionization-mass spectrometry analyses of elgicins AI, AII, and C, these peptides are deduced to have originated from the same precursor, ElgA.ConclusionsA novel lantibiotic-like gene cluster was shown to be present in P. elgii B69. Four new lantibiotics with a broad inhibitory spectrum were isolated, and these appear to be promising antibacterial agents.

Isolation and partial characterization of cyclic lipopeptide antibiotics produced by Paenibacillus ehimensis B7

“…Phylogenetic analysis based on 16S rRNA gene indicated that the isolate was closely related to P. elgii , P. koreensis , and P. tianmuensis (data not shown). This group of bacteria produces diverse antimicrobial agents, including lipopeptides [15,22,23], lantibiotics [24] and macrolide [14]. Interestingly, most extensively described lipopeptide antibiotics from this group of bacteria contain a high percentage of both Dab and a C6-C7 N-terminal fatty acyl chain [15,22].…”

The anti-Staphylococcus aureus activity of the phenanthrene fraction from fibrous roots of Bletilla striata

“…The ethanol extract was loaded onto a polyamide resin column and washed with distilled water, followed by elution with 20, 40, 60, 80, and 95% (v/v) ethanol. Each fraction was collected and tested for antibacterial activity using the agar diffusion method [19]. The tests were repeated three times to ensure reliability.…”

Identification of the biosynthesis gene cluster for the novel lantibiotic paenilan fromPaenibacillus polymyxaE681 and characterization of its product

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