Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (Sj-FABPc) from the human blood fluke Schistosoma japonicum

Becker, Marion; Kalinna, Bernd H.; Waine, G.J.; McManus, Donald P.

doi:10.1016/0378-1119(94)90706-4

Cited by 80 publications

(50 citation statements)

References 24 publications

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“…There are at least two main classes of FABPs, a cytoplasmic and a plasmalemmal FABP (11). Cytoplasmic FABP has been identified in a variety of parasites, including Fasciola hepatica (12), Schistosoma mansoni (5) and S. japonicum (13). Plasmalemmal FABP seems not to be present in trematodes.…”

mentioning

confidence: 99%

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm

Brito

Oliveira²,

Oliveira

et al. 2002

Braz J Med Biol Res

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Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues have a high flow of and demand for lipids, a finding that supports the putative role of Sm14 as an intracellular transporter of fatty acids from host cells.

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mentioning

confidence: 99%

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm

Brito

Oliveira²,

Oliveira

et al. 2002

Braz J Med Biol Res

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“…The overall amino acid sequence of the 19 kDa protein appears similar to that of E. coli amine oxidase (757 amino acid residues; pir 140924) in the region of residues 217^392 (domain of D3^S1) [26], as shown in Fig. 4B, whereas the amino-terminal region (3^49) of the same protein is homologous to the amino-terminal region (1^47) of a fatty acid binding protein (132 amino acid residues; GenBank, L23322) [27] (data not shown). However, it is di¤-cult to rationalize the relationship between the functions and the structural similarities in these examples at present.…”

Section: Structure and Function Of The 35 Kda And 19 Kda Proteinsmentioning

confidence: 81%

Secretional luciferase of the luminous shrimpOplophorus gracilirostris: cDNA cloning of a novel imidazopyrazinone luciferase

Watanabe²,

et al. 2000

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The deep-sea shrimp Oplophorus gracilirostris secretes a luciferase that catalyzes the oxidation of coelenterazine to emit blue light. The luciferase (M r approx. 106 000) was found to be a complex composed of 35 kDa and 19 kDa proteins, and the cDNAs encoding these two proteins were cloned. The expression of the cDNAs in bacterial and mammalian cells indicated that the 19 kDa protein, not the 35 kDa protein, is capable of catalyzing the luminescent oxidation of coelenterazine. The primary sequence of the 35 kDa protein revealed a typical leucine-rich repeat sequence, whereas the catalytic 19 kDa protein shared no homology with any known luciferases including various imidazopyrazinone luciferases. ß

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“…Zhang et al [13] reported that vaccination using a cocktail DNA vaccine induced an increased IgG-specific response against crude worm antigens. SjFABP (encoding a 132-amino acid protein of 14.7 kDa) is found within lipid droplets below the subtegumental region of the male worm, and in the vitelline droplets of the vitelline glands of the female worm; these glands provide nutrients to the developing egg [26,27]. Since schistosomes are unable to synthesize long-chain fatty acids or sterols, and hence are completely dependent on their host for these substances, SjFABP is particularly important to S. japonicum for the uptake, transport and compartmentalisation of host-derived fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine

Li¹,

et al. 2006

Cellular and Molecular Biology Letters

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The aim of this study was to construct and evaluate the immunity efficacy of the DNA multivalent vaccine pVIVO 2 SjFABP-23. The vaccine was constructed and produced as follows. Forty BALB/c mice were divided into four groups designated pVIVO 2 , pVIVO 2 Sj23, pVIVO 2 SjFABP and pVIVO 2 SjFABP-23. Each mouse was immunized with 100 µg of the corresponding plasmid DNA by intramuscular injection. 28 days postvaccination, the mice were challenged with S. japonicum cercariae, and the worm and egg burdens were determined 42 days post-challenge. Serum samples were collected from all the mice before and after vaccination and at the end of the experiment, and used for antibody detection. The IFN-γ and IL-4 levels were quantified in the supernatants of specifically stimulated spleen cells. The number of worms was reduced by 52%, 40% and 42% in mice respectively immunized with pVIVO 2 SjFABP-23, pVIVO 2 Sj23 or pVIVO 2 SjFABP. A respective 61%, 38% and 39% egg reduction was determined relative to those mice that only received the empty pVIVO 2 plasmid. pVIVO 2 SjFABP-23 immunization increased IgG levels against SWAP and SEA. Increased IFN-γ levels were detected in the supernatant of specific stimulated spleen cells from mice immunized with the 3 different constructs. The multivalent DNA vaccine developed induced higher levels of protection than the two monovalent tested vaccines.

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Gene cloning, overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein (Sj-FABPc) from the human blood fluke Schistosoma japonicum

Cited by 80 publications

References 24 publications

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm

Secretional luciferase of the luminous shrimpOplophorus gracilirostris: cDNA cloning of a novel imidazopyrazinone luciferase

Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine

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