Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05

Yan, Jinyong; Yang, Jiangke; Xu, Li; Yan, Yunjun

doi:10.1016/j.molcatb.2007.07.006

Cited by 57 publications

(38 citation statements)

References 33 publications

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“…The presence of K + , Li + , Ag + , and Mg 2+ ions significantly reduced the lipase activity to 70%, 79%, 72%, 65%, respectively. Consistent with previous reports [48][49][50] , the enzyme retained half of its original activity in the presence of Fe 2+ (52%) or Hg 2+ (54%). The 20% inactivation of lipase activity in the presence of PMSF revealed that the bacterial lipase had an active serine residue in its catalytic site thus giving it a property of serine protease 47 .…”

Section: Effects Of Chemicals On Lipase Activitysupporting

confidence: 92%

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Charoenpanich¹,

Suktanarag²,

Toobbucha³

2011

ScienceAsia

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Aeromonas sp. EBB-1 isolated from marine sludge in Angsila, Thailand produced an extracellular thermophilic lipase. Maximum lipase activity of the culture medium was obtained after 15 h at 25°C and pH 8.0 with 0.5% (v/v) olive oil as a carbon source. The enzyme was successfully purified 30-fold to homogeneity by successive ammonium sulphate precipitation, gel-permeation column chromatography, and ultrafiltration. The purified bacterial lipase possessed a relative molecular mass of 45 kDa. Highest lipase activity was determined at pH 8.0 and 37°C with p-nitrophenyl palmitate as a substrate. The enzyme was stable at pH 6.0-8.0 and at temperatures of 30-80°C. Enzyme activity decreased slightly in 50% (v/v) isoamyl alcohol, decane, or heptane. Lipase activity decreased to a half in the presence of butanol and benzene, and by more than 60% in DMSO, methanol, ethanol, and hexadecane. Lipase activity was inhibited by most of the salt ions (except Ca 2+ ions) as well as by Tween 80, DTT, PMSF, SDS, and EDTA. Although lipase showed variable specificity/hydrolytic activity towards a number of p-nitrophenyl esters, it was preferentially active towards long-carbon chain acyl esters (C12-C16). The high temperature stability and ability to hydrolyse long length esters support the potential of this lipase enzyme as a vigorous biocatalyst for industrial applications.

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Section: Effects Of Chemicals On Lipase Activitysupporting

confidence: 92%

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Charoenpanich¹,

Suktanarag²,

Toobbucha³

2011

ScienceAsia

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“…lipase genes also in P. pastoris, obtained (273 ± 2.4) μmol FFA min -1 g -1 of dry cells. In addition, Galactomyces geotrichum lipases have also been successfully expressed, mostly in Picchia pastoris 28,70 . In all these examples, the lipase activity or lipid contents in the clones or mutants of Geotrichum were much higher compared to original strains, indicating that these techniques can used successfully in industrial applications that need a high level of lipases activity.…”

Section: Production Of Geotrichum Spp Lipasesmentioning

confidence: 99%

“…Lipases from Geotrichum candidum are known for their high specificity for unsaturated long chain fatty acids and esters, while lipases from Galactomyces geotrichum present specificity for other esters of long chain fatty acids and good stability regarding temperature, pH and organic solvents. These properties allow their utilization in different reactions utilizing various vegetable oils 22,25,[27][28][29] . These microorganisms have been isolated from soil and plants 29,30 , dairy products 31,32 , rotten vegetables and fruits 33 , different infections 34 , and many other sources 35 .…”

Section: Introductionmentioning

confidence: 99%

A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

Maldonado

Lopes

Aguiar‐Oliveira

et al. 2017

Chem.Biochem.Eng.Q.

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Lipases are enzymes produced from innumerous microorganisms, plants and animal cells. They catalyze reactions of different lipid sources. The Geotrichum fungi are good producers of lipases with high hydrolytic activity and specificity for unsaturated fatty acids. A great number of studies have reported the importance of lipase from this genus and described important fermentation parameters for the enzyme production, such as nutrients, temperature, pH, inoculum, time of fermentation and others. Furthermore, different strategies have been used to purify and immobilize lipases from Geotrichum and innumerous applications are cited in different processes as polyunsaturated fatty acids enrichment, hydrolysis and esterification of fat and oils, synthesis of aromas, biodiesel, and many others. This review highlights fundamental aspects of the production, purification, characterization, immobilization, and the applications of lipases produced by the genus Geotrichum.

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“…Yan et al (2007) made cloning and purification of a lipase from G. geotrichum to know the enzyme potential for biodiesel production, selective hydrolysis, esterification for enrichment in PUFAs and for oil-contaminated biodegradation. A lipase isolated from G. geotrichum Y05 strain is thermostable and tolerant to organic solvents, therefore, it can be used as an excellent industrial catalyst (Yan et al, 2007).…”

Section: Biodegradation and Bioremediation Properties Of Galactomycesmentioning

confidence: 99%

Galactomyces geotrichum – moulds from dairy products with high biotechnological potential

Grygier

Myszka

Rudzińska

2017

Acta Sci Pol Technol Aliment

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The article reviews the properties of the Galactomyces geotrichum species, the mould that is most important for the dairy industry. G. geotrichum mould has been isolated from milk, cheeses and alcoholic beverage. Its presence in food products makes it possible to obtain a characteristic aroma and taste, which corresponds to the needs and preferences of consumers. G. geotrichum plays an important role in ecology, where the mould is employed for the degradation of various hazardous substances and wastewater treatment. It has also been found to have potential for biofuel production. In addition to this, G. geotrichum can be applicable in two further major areas: agriculture and health protection.

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Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05

Cited by 57 publications

References 33 publications

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A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

Galactomyces geotrichum – moulds from dairy products with high biotechnological potential

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Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05

Cited by 57 publications

References 33 publications

Untitled

Untitled

A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

Galactomyces geotrichum &#8211; moulds from dairy products with high biotechnological potential

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Galactomyces geotrichum – moulds from dairy products with high biotechnological potential