Gene-based molecular marker system for multiple disease resistances in tomato against Tomato yellow leaf curl virus, late blight, and verticillium wilt

Jung, Jungsu; Kim, Hyun Jung; Lee, Je Min; Oh, Chang‐Sik; Lee, Hyung‐Jin; Yeam, Inhwa

doi:10.1007/s10681-015-1442-z

Cited by 26 publications

(18 citation statements)

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“…The Ty -1 gene is located on chromosome 6 in a genomic region in repulsion phase linkage with resistance genes against other pathogens, including the Mi -1.2 gene that confers resistance in tomato to the three most important root-knot nematode species: Meloidogyne incognita, Meloidogyne javanica, and Meloidogyne arenaria [ 27 , 28 ]. Molecular markers for monitoring the presence of the Ty -1 gene/locus in tomato cultivars are now available [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Reis

Fonseca

Ribeiro

et al. 2020

Viruses

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A complex of begomoviruses (Geminiviridae) can cause severe tomato yield losses in the neotropics. Here, next-generation sequencing was employed for large-scale assessment of single-stranded (ss)DNA virus diversity in tomatoes either harboring or lacking the large-spectrum begomovirus tolerance Ty-1 gene. Individual leaf samples exhibiting begomovirus-like symptoms (n = 107) were field-collected, circular DNA-enriched, subdivided into pools (with and without Ty-1), and Illumina-sequenced. Virus-specific PCR and Sanger dideoxy sequencing validations confirmed 15 distinct ssDNA virus/subviral agents (occurring mainly in mixed infections), which highlight the potential drawbacks of employing virus-specific resistance in tomato breeding. More viruses (14 versus 6 species) were observed in tomatoes without the Ty-1 gene. A gemycircularvirus (Genomoviridae), a new alpha-satellite, and two novel Begomovirus species were identified exclusively in samples without the Ty-1 gene. A novel begomovirus was found only in the Ty-1 pool, being the only species associated with severe symptoms in Ty-1 plants in our survey. Our work is the first step towards the elucidation of the potential begomovirus adaptation to Ty-1 and its specific filtering effects on a subset of ssDNA viral/subviral agents.

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Section: Introductionmentioning

confidence: 99%

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Reis

Fonseca

Ribeiro

et al. 2020

Viruses

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“…In contrast to common molecular markers based on DNA polymorphisms in regions flanking of the gene of interest, gene-based markers markedly enhance the efficiency of MAS (Salgotra et al 2014). Since the Ty-1/Ty-3 gene was identified, four gene-based markers have been developed (Jung et al 2015). Three of them are codominant CAPS markers, and one is a dominant HRM marker.…”

Section: Discussionmentioning

confidence: 99%

“…Gene-based molecular markers of Ty-3 would be useful to predict the phenotypic variations. There have been gene-based markers developed for Ty-1/Ty-3 by comparative analysis of tomato accessions (Jung et al 2015;Lee et al 2015). In the present study, we developed Ty-3 gene-based co-dominant markers in a bi-parental population to improve the efficiency of genotyping and to increase the diversity of molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance GeneTy-3

Dong¹,

Han²,

Siddique³

et al. 2016

Plant Breed. Biotech

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2016 (February) ABSTRACT The viral disease induced by Tomato yellow leaf curl virus (TYLCV) reduces tomato (Solanum lycopersicum) yield significantly in tropical and subtropical regions. A number of loci, including Ty-1 to Ty-5, conferring resistance to TYLCV have been described and introgressed into modern tomato cultivars. The availability of molecular markers linked to these genes would expedite the introgression of TYLCV resistance into commercial cultivars. In the present study, we developed gene-based markers linked to the Ty-3 gene using a segregating population derived from a cross between the TYLCV-resistant line S. lycopersicum 'A45' and the susceptible line S. lycopersicum 'A39'. Agrobacterium-mediated screening was used to test TYLCV resistance of plants in the segregating population, and the resistance was evaluated by a visual scoring method and polymerase chain reaction analysis. By comparing sequences of the Ty-3 genes of the resistant and susceptible lines, two high-resolution melting (HRM) markers (Ty3-HRM1 and Ty3-HRM2) and one sequence characterized amplified region (SCAR) marker (Ty3-SCAR1) were developed. The HRM markers were based on single nucleotide polymorphisms at the 13th exon and the 15th intron, whereas the SCAR marker was based on a 246-bp deletion in the 16th intron. These gene-based markers will be useful tools for marker-assisted selection in breeding programs to improve TYLCV resistance of tomato.

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“…Increased access to genomic information has led to a considerable number of gene-based markers for disease resistances, which are greatly advantageous compared with neutral markers linked to the genes of interest (Kage et al, 2015;Kamphuis et al, 2015). PCR-gel based systems using cleaved and amplified polymorphic sequence (CAPS) markers and high-throughput SNP detection systems using high resolutionmelt (HRM) markers have been utilized widely to detect multiple SNPs associated with disease-resistance traits (Lochlainn et al, 2011;Jung et al, 2015). Currently, several advanced, high- Abbreviations: eIF4E (eukaryotic translation initiation factor 4E), eIF(iso)4E (eukaryotic translation initiation factor iso 4E), Pelo (a messenger RNA surveillance factor), VPg (genome linked viral protein), CPR (constitutive expresser of pathogenesis related genes), CI-Cter (C terminal of cylindrical inclusion helicase)…”

Section: Applications Of Natural Virus Resistance: Molecular Breedingmentioning

confidence: 99%

Current advances and prospectus of viral resistance in horticultural crops

Yeam

2016

Hortic. Environ. Biotechnol.

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Abstract. Viruses are a major threat causing massive yield loss and economical damage to crop production worldwide. Through complex evolutionary processes, plants encounter and overcome viral infection by developing effective resistance mechanisms. Over the past decade, remarkable progress has been made in understanding the nature of plant resistance to viruses at the molecular level. This review summarizes the major resistance strategies that plants use to prevent viral infection. Recent investigations suggest that antiviral RNA silencing is the most prevalent defense strategy in plants.Other forms of resistance include R gene-mediated resistance and host factor-related recessive resistance. Naturally occurring resistances arise and are maintained in numerous virus-plant pathosystems based mainly on arms-race relationships and the cost-efficiency of resistance acquisition. In addition to the current status of the known resistance mechanisms, this review discusses the future prospectus for the practical application of plant resistances that influence resistance durability in agricultural ecosystems. Such applications include molecular breeding strategies using advanced molecular marker systems and the utilization of trans-or cis-genetics via the acquisition of engineered disease resistances.

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Gene-based molecular marker system for multiple disease resistances in tomato against Tomato yellow leaf curl virus, late blight, and verticillium wilt

Cited by 26 publications

References 50 publications

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Metagenomics of Neotropical Single-Stranded DNA Viruses in Tomato Cultivars with and without the Ty-1 Gene

Gene-Based Markers for the Tomato Yellow Leaf Curl Virus Resistance GeneTy-3

Current advances and prospectus of viral resistance in horticultural crops

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