“…The technology is based on a combination of microemulsion PCR on magnetic beads followed by allele-specific oligonucleotide hybridization and laser-induced fluorescence detection on a flow cytometer . On the other hand, end-point bio(chemi)luminometric assays have found wide applications in DNA/RNA assays since they provide higher detectability, wider linear range, high throughput, and simpler instrumentation (no need for excitation light). − Enzymes, along with chemiluminogenic substrates, or photoproteins have been employed as reporters in hybridization assays for the determination of PCR-amplified DNA, − determination of microRNA, and quantitative competitive PCR. , Bio(chemi)luminometric assays for genotyping of point mutations (e.g., single-nucleotide polymorphisms) comprise three steps: (i) exponential amplification, usually by PCR, (ii) an allele-discrimination reaction, e.g., primer extension reaction , or oligonucleotide ligation reaction, and (iii) detection of the reaction product by exploiting a bio(chemi)luminescent reporter. However, the aforementioned assays simply discriminate the three genotypes (normal, homozygote, and heterozygote) in inherited mutations without quantifying the amount of the mutant allele in the sample.…”