Gene and Cell Therapy in Germany and the EU

Cichutek, Klaus

doi:10.1007/s00003-008-0323-y

Cited by 5 publications

(2 citation statements)

References 12 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present study used genetically modified NSCs overexpressing GDNF or BDNF neutotrophic factors to examine sciatic nerve regeneration across a long nerve defect. Although genetic manipulation and cell therapy exhibit good effects on curing spinal cord and peripheral nerve injury in animal models, most of them are difficult, risky, or ethically problematic to transfer to the clinic (21). The nonviral transfection was chosen as it reduced the risk of ectotopic and uncontrolled gene expression compared with direct injection.…”

Section: Discussionmentioning

confidence: 99%

Sciatic Nerve Regeneration by Microporous Nerve Conduits Seeded With Glial Cell Line‐Derived Neurotrophic Factor or Brain‐Derived Neurotrophic Factor Gene Transfected Neural Stem Cells

Dai

Chiu

et al. 2011

Artificial Organs

View full text Add to dashboard Cite

Neurotrophic factors such as the glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF) promote nerve cell survival and regeneration, but their efficacy in repairing a longer gap defect of rat sciatic nerve (15 mm) has not been established. In this study, two recombinant mammalian vectors containing either rat GDNF gene or BDNF gene were constructed and each was transfected into neural stem cells (NSCs). It was found that the transfection of GDNF or BDNF gene into NSCs led to significantly enhanced expression of GDNF or BDNF mRNA. The amount of GDNF or BDNF protein secreted from the transfected NSCs showed a 3.3-fold or 2.5-fold increase than that from nontransfected NSCs, respectively. The regeneration capacity of rat sciatic nerve in a poly(D,L-lactide) conduit seeded with GDNF or BDNF-transfected NSCs was evaluated by the histology, functional gait, and electrophysiology after 8 weeks of implantation. It was observed that the degree of myelination and the size of regenerated tissue in the conduits seeded with GDNF- and BDNF-transfected NSCs were higher than those seeded with the nontransfected NSCs. Conduits seeded with GDNF-transfected NSCs had the greatest number of blood vessels. The functional recovery assessed by the functional gait and electrophysiology was significantly improved for conduits seeded with GDNF or BDNF-transfected NSCs. It was concluded that the genetically modified NSCs may have potential applications in promoting nerve regeneration and functional recovery.

show abstract

Section: Discussionmentioning

confidence: 99%

Sciatic Nerve Regeneration by Microporous Nerve Conduits Seeded With Glial Cell Line‐Derived Neurotrophic Factor or Brain‐Derived Neurotrophic Factor Gene Transfected Neural Stem Cells

Dai

Chiu

et al. 2011

Artificial Organs

View full text Add to dashboard Cite

show abstract

“…Andererseits überwiegen die Forschungskapazitäten auf den Sektoren der Zell-und Stammzell-Therapie bereits die Studien, die langfristig zur Somatischen Gentherapie in Deutschland betrieben werden. Regenerative Ansätze mit pluripotenten Stamm-Zellen finden sowohl in der Cardiologie, der Hepatologie, der Neurologie als auch beim Knochen-und Stützgewebe den Zugang zu klinischen Studien oder Verträglichkeitsprüfungen (Acikgoez und Bader, 2008;Cichutek, 2008;Yerebakan et al, 2008).…”

unclassified

5. Berliner Genetik-Workshop

Grugel

2008

J. Verbr. Lebensm.

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer

Gevaert

Bracke

et al. 2017

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer. Manuscript submitted (2016). Acknowledgement: China Scholarship Council (CSC)  A HILIC method for the analysis of HEPES was developed, validated and applied to commercial products from different suppliers.  The influence of different factors on retention time was investigated to get a better understanding of the retention.  The obtained data were evaluated by different retention models and a mixed model of partition and adsorption mechanism was found to fit best, with adsorption being the main retention mechanism, combined with partition and ion exchange mechanisms.  The stress test of HEPES found one degradant under oxidative conditions, identified as N-oxide by high resolution MS.

show abstract

Gene and Cell Therapy in Germany and the EU

Cited by 5 publications

References 12 publications

Sciatic Nerve Regeneration by Microporous Nerve Conduits Seeded With Glial Cell Line‐Derived Neurotrophic Factor or Brain‐Derived Neurotrophic Factor Gene Transfected Neural Stem Cells

Sciatic Nerve Regeneration by Microporous Nerve Conduits Seeded With Glial Cell Line‐Derived Neurotrophic Factor or Brain‐Derived Neurotrophic Factor Gene Transfected Neural Stem Cells

5. Berliner Genetik-Workshop

Hydrophilic interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer

Contact Info

Product

Resources

About