Gene and Cell Therapy for Heart Failure

Muinck, Ebo D. de

doi:10.1089/ars.2009.2495

Cited by 7 publications

(11 citation statements)

References 142 publications

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“…Adenoviruses , which are non-enveloped and contain linear double-stranded DNA, can be easily manipulated and will accept large inserts up to 35 kilobases in size [100]. Adenoviruses can infect host cells with very high efficiency and are capable of generating high levels of gene expression [78].…”

Section: Gene Transfermentioning

confidence: 99%

“…They can readily infect cardiac cells resulting in stable gene expression for up to one year [114], and since they are not known to cause any human disease, very little immune response is triggered [100]. For these reasons, AAV are considered excellent candidates for use in gene therapy in humans [106].…”

Section: Gene Transfermentioning

confidence: 99%

“…However, lentiviruses , which are a genus of the retrovirus family, can multiply in non-dividing cells [117], making them suitable for use in adult [80, 118] as well as neonatal [80, 117–119] cardiomyocytes. Importantly, lentiviruses do not induce inflammatory and immune responses, and can induce long duration expression by incorporating the transgene into the host genome [100]. These viruses can accommodate a relatively large (up to 18 kb [120]) insert, which makes them better suited for the transfer of large genes than AAV.…”

Section: Gene Transfermentioning

confidence: 99%

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Methods in cardiomyocyte isolation, culture, and gene transfer

Louch

Sheehan

Wolska

2011

Journal of Molecular and Cellular Cardiology

420

396

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Since techniques for cardiomyocyte isolation were first developed nearly 35 years ago, experiments on single myocytes have yielded great insight into their cellular and sub-cellular physiology. These studies have employed a broad range of techniques including electrophysiology, calcium imaging, cell mechanics, immunohistochemistry and protein biochemistry. More recently, techniques for cardiomyocyte culture have gained additional importance with the advent of gene transfer technology. While such studies require a high quality cardiomyocyte population, successful cell isolation and maintenance during culture remain challenging. In this review, we describe methods for the isolation of adult and neonatal ventricular myocytes from rat and mouse heart. This discussion outlines general principles for the beginner, but also provides detailed specific protocols and advice for common caveats. We additionally review methods for short-term myocyte culture, with particular attention given to the importance of substrate and media selection, and describe time-dependent alterations in myocyte physiology that should be anticipated. Gene transfer techniques for neonatal and adult cardiomyocytes are also reviewed, including methods for transfection (liposome, electroporation) and viral-based gene delivery.

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Section: Gene Transfermentioning

confidence: 99%

Section: Gene Transfermentioning

confidence: 99%

Section: Gene Transfermentioning

confidence: 99%

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Methods in cardiomyocyte isolation, culture, and gene transfer

Louch

Sheehan

Wolska

2011

Journal of Molecular and Cellular Cardiology

420

396

View full text Add to dashboard Cite

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“…In recent years, stem cell technology is gaining pace in the management and regenerative therapy of heart failure. 2 Research on cell-based regenerative therapy has focused on identifying an ideal cellular source. The optimal cell source to create an engineered myocardial patch should be easy to harvest, proliferate under controlled conditions, nonimmunogenic and should have the ability to differentiate into mature, functional cardiomyocyte.…”

Section: Progress and Challenges In Stem Cell Regenerative Therapymentioning

confidence: 99%

Regenerative therapy and tissue engineering for the treatment of end-stage cardiac failure

Finosh

Jayabalan

2012

Biomatter

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“…A number of different vector systems are already available, but each of them has limiting drawbacks [106]. A number of different vector systems are already available, but each of them has limiting drawbacks [106].…”

Section: Gene Therapy and I/rmentioning

confidence: 99%

Cell Survival Programs and Ischemia /Reperfusion: Hormesis, Preconditioning, and Cardioprotection

Krenz¹,

Baines²,

Kalogeris³

et al. 2013

Colloquium Series on Integrated Systems Physiology: From Molecule to Function

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ii This e-book is an original work commissioned for the Colloquium Digital Library of Life Sciences, a curated collection of time-saving pedagogical resources for researchers and students who want to quickly get up to speed in a new area of life science/biomedical research. Each e-book available in Colloquium is an in-depth overview of a fast-moving or fundamental area of research, authored by a prominent contributor to the field. We call these resources 'Lectures' because authors are asked to provide an authoritative, state-of-the-art overview of their area of expertise, in a manner that is accessible to a broad, diverse audience of scientists (similar to a plenary or keynote lecture at a symposium/meeting/colloquium). Readers are invited to keep current with advances in various disciplines, gain insight into fields other than their own, and refresh their understanding of core concepts in cell & molecular biology. Physiology is a scientific discipline devoted to understanding the functions of the body. It addresses function at multiple levels, including molecular, cellular, organ, and system. An appreciation of the processes that occur at each level is necessary to understand function in health and the dysfunction associated with disease. Homeostasis and integration are fundamental principles of physiology that account for the relative constancy of organ processes and bodily function even in the face of substantial environmental changes. This constancy results from integrative, cooperative interactions of chemical and electrical signaling processes within and between cells, organs and systems. This eBook series on the broad field of physiology covers the major organ systems from an integrative perspective that addresses the molecular and cellular processes that contribute to homeostasis. Material on pathophysiology is also included throughout the eBooks. The state-of the-art treatises were produced by leading experts in the field of physiology. Each eBook includes stand-alone information and is intended to be of value to students, scientists, and clinicians in the biomedical sciences. Since physiological concepts are an ever-changing work-in-progress, each contributor will have the opportunity to make periodic updates of the covered material.Published titles (for future titles please see the website, www.morganclaypool.com/page/lifesci) ABSTRACTThe major purpose of this book is to review the evidence supporting the concept that intrinsic cell survival programs can be activated by a variety of mildly noxious stimuli or pharmacologic agents to confer protection against the deleterious effects of ischemia/reperfusion (I/R). We begin with a discussion of the concept of hormesis (a term used most extensively in the toxicologic literature which refers to biphasic cellular responses that depend on concentration or intensity of a stimulus), review the seminal studies that led to the discovery of the cardioprotective effects of ischemic preconditioning, and outline its therapeutic potential (Chapter 1). This is followed ...

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Gene and Cell Therapy for Heart Failure

Cited by 7 publications

References 142 publications

Methods in cardiomyocyte isolation, culture, and gene transfer

Methods in cardiomyocyte isolation, culture, and gene transfer

Regenerative therapy and tissue engineering for the treatment of end-stage cardiac failure

Cell Survival Programs and Ischemia /Reperfusion: Hormesis, Preconditioning, and Cardioprotection

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