Gene and Cell Replacement via Neural Stem Cells

Kim, Hyoung Tai; Kim, Il Sun; Lim, Seung Eun; Lee, Il Shin; Park, Kook In

doi:10.3349/ymj.2004.45.suppl.32

Cited by 10 publications

(5 citation statements)

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“…Neural stem cells (NSCs) are self-renewing, multipotent cells that generate the main phenotype of the central nervous system. NSCs also undergo asymmetric cell division into two daughter cells, wherein one is specialized and the other is a nonspecialized subtype [ 21 , 22 ]. NSCs primarily differentiate into astrocytes, and oligodendrocytes, and neurons and show the corresponding cell phenotype, morphological structure, and biological features [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

Duan

Han

et al. 2016

BioMed Research International

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Objective. To investigate the effect of advanced motherhood on rat hippocampal neural stem cell proliferation. Methods. Female parents were subdivided into control and old mother group by age, and neural stem cells were cultured from hippocampal tissues for 24 h newborn offspring. The diameter and numbers of neurospheres were examined by microscopy, and differences in proliferation were examined by EdU immunofluorescence, CCK-8 assay, and cell cycle analysis. Results. The number of neurospheres in the old mother group after culture was lower than the control group. Additionally, neurospheres' diameter was smaller than that of the control group (P < 0.05). The EdU positive rate of the old mother group was lower than that of the control group (P < 0.05). CCK-8 assay results showed that the absorbance values for the old mother group were lower than that of the control group at 48 h and 72 h (P < 0.05). The proportions of cells in the S and G2/M phases of the cell cycle for the older mother group were less than that found for the control group (P < 0.05). Conclusion. The proliferation rates of hippocampal NSCs seen in the older mother group were lower than that seen in the control group.

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Section: Discussionmentioning

confidence: 99%

The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

Duan

Han

et al. 2016

BioMed Research International

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“…Human NSC have been immortalized by using vectors carrying v-myc (26,96,198,199). Among these cell lines, some have been shown to express electrophysiological properties of functional neurons and to respond to neurotransmitters in vitro (190), to promote functional recovery in animal models (48,94), and to integrate into the adult intact rat brain (161). Other cell lines, while showing functionality in vitro, have failed to improve behavioral deficits in hemiparkinsonian rats (142).…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Strategies for the Development of Cell Lines for Ex Vivo Gene Therapy in the Central Nervous System

Mejía-Toiber

Castillo²,

Giordano

2011

Cell Transplant

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Disorders of the central nervous system (CNS) as a result of trauma or ischemic or neurodegenerative processes still pose a challenge for modern medicine. Due to the complexity of the CNS, and in spite of the advances in the knowledge of its anatomy, pharmacology, and molecular and cellular biology, treatments for these diseases are still limited. The development of cell lines as a source for transplantation into the damaged CNS (cell therapy), and more recently their genetic modification to favor the expression and delivery of molecules with therapeutic potential (ex vivo gene therapy), are some of the techniques used in search of novel restorative strategies. This article reviews the different approaches that have been used and perfected during the last decade to generate cell lines and their use in experimental models of neuronal damage, and evaluates the prospects of applying these methods to treat CNS disorders.

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“…When cultured in vitro in an appropriate culture conditions, ES cells spontaneously differentiate into specialized cells such as cardiac [1][2][3][4][5], neural [6][7][8][9][10], hematopoietic [11][12][13][14][15], vascular smooth muscle [16; 17] endodermal [18; 19], adipogenic [20; 21] and chondrogenic [22]. This potential to differentiate into all cell types, known as pluripotency makes human ES cells unique for applications in cell transplantation therapy and other prospective areas of regenerative medicine.…”

Section: Es Cells Are Pluripotentmentioning

confidence: 99%

Prospects of Embryonic Stem Cells in Treatment of Hematopoietic Disorders

Srivastava

Malhotra²,

Esmaeli-Azad³

et al. 2007

CPB

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Cellular therapies derived from embryonic stem (ES) cells have gained a renewed interest with the experimental demonstration that an embryonic stem cell lines can be established from human blastocyst-stage embryos and prompted to differentiate into almost all types of cells present in the body including hematopoietic cells. Hematopoiesis is a series of cellular processes whereby short-lived mature blood cells are continuously replenished from a pool of rare pluripotential hematopoietic stem cells, in a highly orchestrated process. Aberrances in this intricate process may lead to a malignancy of essential blood-forming organs, causing diseases such as leukemia, aplastic anemia, lymphoma, myelodysplasia and myeloproliferative disorders. Embryonic stem cells show great potential and it may be technologically feasible to transplant differentiated ES cells and to cure various kinds of blood disorders. Understanding the biology of ES cell derived hematopoiesis may lead to the development of co-transplantation protocols that will result in a decreased morbidity and mortality by providing safer and simpler transplantation procedures for patients with malignant and non-malignant conditions. The potential utility of ES cells for gene therapy, tissue engineering and the treatment of a wide variety of currently untreatable diseases is simply too essential to ignore, however, our knowledge and ability to deliver these forms of therapy in a safe and efficient manner requires additional advances in the understanding of the basic biology of ES cells. In this article, we will discuss the factors and methodologies responsible for the differentiation of ES cells into hematopoietic progenitors and their potential to treat different blood related diseases.

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Gene and Cell Replacement via Neural Stem Cells

Cited by 10 publications

References 0 publications

The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

The Effect of Advanced Motherhood on Newborn Offspring’s Hippocampal Neural Stem Cell Proliferation

Strategies for the Development of Cell Lines for Ex Vivo Gene Therapy in the Central Nervous System

Prospects of Embryonic Stem Cells in Treatment of Hematopoietic Disorders

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