Gene Analysis of Panicle Blast Resistance in Rice Cultivars with Rice Stripe Resistance.

Fujii, Kiyoshi; Hayano-Saito, Yuriko; Sugiura, Naoki; Hayashi, Nagao; Saka, Norikuni; Tooyama, Takamichi; Izawa, Toshihiko; Shumiya, A.

doi:10.1270/jsbbr.1.203

Cited by 25 publications

(16 citation statements)

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“…The Pb1 locus has previously been mapped to a Modan‐derived chromosomal region in the middle part of the long arm of chromosome 11 in japonica cultivars (Fujii et al. , 1999, 2000).…”

Section: Resultsmentioning

confidence: 99%

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Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication

Hayashi

Inoue

Kato³

et al. 2010

The Plant Journal

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234

161

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Rice blast is one of the most widespread and destructive plant diseases worldwide. Breeders have used disease resistance (R) genes that mediate fungal race-specific 'gene-for-gene' resistance to manage rice blast, but the resistance is prone to breakdown due to high pathogenic variability of blast fungus. Panicle blast 1 (Pb1) is a blast-resistance gene derived from the indica cultivar 'Modan'. Pb1-mediated resistance, which is characterized by durability of resistance and adult/panicle blast resistance, has been introduced into elite varieties for commercial cultivation. We isolated the Pb1 gene by map-based cloning. It encoded a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. The Pb1 protein sequence differed from previously reported R-proteins, particularly in the NBS domain, in which the P-loop was apparently absent and some other motifs were degenerated. Pb1 was located within one of tandemly repeated 60-kb units, which presumably arose through local genome duplication. Pb1 transcript levels increased during the development of Pb1+ cultivars; this expression pattern accounts for their adult/panicle resistance. Promoter:GUS analysis indicated that genome duplication played a crucial role in the generation of Pb1 by placing a promoter sequence upstream of its coding sequence, thereby conferring a Pb1-characteristic expression pattern to a transcriptionally inactive 'sleeping' resistance gene. We discuss possible determinants for the durability of Pb1-mediated blast resistance.

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“…The Pb1 locus has previously been mapped to a Modan‐derived chromosomal region in the middle part of the long arm of chromosome 11 in japonica cultivars (Fujii et al. , 1999, 2000).…”

Section: Resultsmentioning

confidence: 99%

“…A blast‐resistance gene, Panicle blast 1 ( Pb1 ), was found in japonica rice cultivars ‘Tsukinohikari’ and ‘Asanohikari’ at a single chromosomal region derived from the indica cultivar ‘Modan’ (Fujii et al. , 1999).…”

Section: Introductionmentioning

confidence: 99%

Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication

Hayashi

Inoue

Kato³

et al. 2010

The Plant Journal

Self Cite

234

161

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“…On the other hand, partial resistance reduces the extent of pathogen reproduction in the compatible interaction (Jonson 1983;Parlevliet 1988). However, there are some exceptions such as Pif (Toriyama et al 1968) and Pb1(t) (Fujii et al 1999), which are single genes conferring partial resistance to blast. However, there are some exceptions such as Pif (Toriyama et al 1968) and Pb1(t) (Fujii et al 1999), which are single genes conferring partial resistance to blast.…”

Section: Introductionmentioning

confidence: 99%

Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32

et al. 2002

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The rice cultivar Chubu 32 possesses a high level of partial resistance to leaf blast. The number and chromosomal location of genes conferring this resistance were detected by restriction fragment length polymorphism (RFLP) linkage mapping and quantitative trait locus (QTL) analysis. For the mapping, 149 F(3) lines derived from the cross between rice cultivar Norin 29, with a low level of partial resistance, and Chubu 32 were used, and their partial resistance to leaf blast was assessed in upland nurseries. A linkage map covering six chromosomes and consisting of 36 RFLP markers was constructed. In the map, only one significant QTL (LOD>2.0) for partial resistance was detected on chromosome 11. This QTL explained 45.6% of the phenotypic variation. The segregation ratio of the F3 lines was 3:1 for partial resistance to susceptibility. These results suggest that the partial resistance in Chubu 32 is controlled by a major gene.

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“…However, 25 years has elapsed since the first crossing of a japonica variety to ‘Rantaj‐emas2’. One of the reasons why such a long period has been required is that ‘Daichino‐kaze’ is a pyramid variety to which rice stripe resistance gene Stv‐b i from a Pakistan variety ‘Modan’ and rice blast resistance gene Pb1 (Fujii et al. 1999) have been introduced.…”

Section: Discussionmentioning

confidence: 99%

Development of cleaved amplified polymorphic sequence (CAPS) markers linked to a green rice leafhopper resistance gene, Grh3(t)

Saka¹,

Tsuji²,

Toyama³

et al. 2006

Plant Breeding

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The chromosomal location of the resistance gene for green rice leafhopper (GRLH), an injurious insect for rice, has been determined and RFLP markers closely linked to this gene have been identified. The susceptible japonica rice variety ÔNipponbareÕ was crossed with a resistant japonica rice line ÔAichi42Õ, in which green rice leaf hopper resistance had been introduced from an indica variety ÔRantaj-emas2Õ, and the 100 F 2 plants obtained were used for linkage analysis. The green rice leafhopper resistance gene, Grh3(t), was mapped between RFLP markers C288B and C133A on chromosome 6 and co-segregated with C81. Of the RFLP markers tightly linked to Grh3(t), C81 was converted to a SCAR marker and C133A to a cleaved amplified polymorphic sequence marker that could distinguish the heterozygous genotype to establish an effective marker-aided selection system for the GRLH resistance gene.

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Gene Analysis of Panicle Blast Resistance in Rice Cultivars with Rice Stripe Resistance.

Cited by 25 publications

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Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication

Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication

Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32

Development of cleaved amplified polymorphic sequence (CAPS) markers linked to a green rice leafhopper resistance gene, Grh3(t)

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