Gender-specific metabolic responses in hepatopancreas of mussel Mytilus galloprovincialis challenged by Vibrio harveyi

Liu, Xiaoli; Sun, Hushan; Wang, Yiyan; Ma, Mengwen; Zhang, Yuemei

doi:10.1016/j.fsi.2014.08.002

Cited by 32 publications

(15 citation statements)

References 33 publications

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“…Impacted metabolites in male mussels (e.g., threonine, glutamate, lysine, and histidine) suggest a change in energy metabolism, while a disturbance of both osmoregulation and energy metabolism is rather demonstrated in females (modulation of the osmolytes betaine and hypotaurine, ATP elevation). Other studies in environmental metabolomics also demonstrated the gender-specific response of mussels exposed to the pesticide triazophos [ 19 ], the flame retardant tetrabromobisphenol A [ 20 ], bisphenol A [ 21 ], and the pharmaceutical active compound fadrozole hydrochloride [ 22 ], or challenged by pathogens [ 23 , 24 ]. However, those studies did not fully take advantage of the multifactorial experimental structure of the data to characterize both exposure effects and gender-specific responses and to relate the observed variability (metabolic changes) to the main factors or their interaction in a multivariate context.…”

Section: Introductionmentioning

confidence: 99%

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

et al. 2020

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Environmental metabolomics is a powerful approach to investigate the response of organisms to contaminant exposure at a molecular scale. However, metabolomic responses to realistic environmental conditions can be hindered by factors intrinsic to the environment and the organism. Hence, a well-designed experimental exposure associated with adequate statistical analysis could be helpful to better characterize and relate the observed variability to its different origins. In the current study, we applied a multifactorial experiment combined to Analysis of variance Multiblock Orthogonal Partial Least Squares (AMOPLS), to assess the metabolic response of wild marine mussels, Mytilus galloprovincialis, exposed to a wastewater treatment plant effluent, considering gender as an experimental factor. First, the total observed variability was decomposed to highlight the contribution of each effect related to the experimental factors. Both the exposure and the interaction gender × exposure had a statistically significant impact on the observed metabolic alteration. Then, these metabolic patterns were further characterized by analyzing the individual variable contributions to each effect. A main change in glycerophospholipid levels was highlighted in both males and females as a common response, possibly caused by oxidative stress, which could lead to reproductive disorders, whereas metabolic alterations in some polar lipids and kynurenine pathway were rather gender-specific. This may indicate a disturbance in the energy metabolism and immune system only in males. Finally, AMOPLS is a useful tool facilitating the interpretation of complex metabolomic data and is expected to have a broad application in the field of ecotoxicology.

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Section: Introductionmentioning

confidence: 99%

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

et al. 2020

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“…In crustaceans, the functions of the hepatopancreas include carbohydrate and lipid metabolism, oxidative stress, energy storage and breakdown (Wang et al, 2008 ). Therefore, we hypothesized that the M. nipponense hepatopancreas undergoes marked metabolomic changes in response to hypoxia (Liu et al, 2014 ; Song Q. et al, 2017 ). Nonetheless, the specific mechanisms by hepatopancreas of prawns respond to hypoxia stress are largely unknown and hypoxia-related metabolomics information remains limited.…”

Section: Introductionmentioning

confidence: 99%

Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Sun

Guo

et al. 2018

Front. Physiol.

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Hypoxia represents a major physiological challenge for prawns and is a problem in aquaculture. Therefore, an understanding of the metabolic response mechanism of economically important prawn species to hypoxia and re-oxygenation is essential. However, little is known about the intrinsic mechanisms by which the oriental river prawn Macrobrachium nipponense copes with hypoxia at the metabolic level. In this study, we conducted gas chromatography-mass spectrometry-based metabolomics studies and assays of energy metabolism-related parameters to investigate the metabolic mechanisms in the hepatopancreas of M. nipponense in response to 2.0 O2/L hypoxia for 6 and 24 h, and reoxygenation for 6 h following hypoxia for 24 h. Prawns under hypoxic stress displayed higher glycolysis-related enzyme activities and lower mRNA expression levels of aerobic respiratory enzymes than those in the normoxic control group, and those parameters returned to control levels in the reoxygenated group. Our results showed that hypoxia induced significant metabolomic alterations in the prawn hepatopancreas within 24 h. The main metabolic alterations were depletion of amino acids and 2-hydroxybutanoic acid and accumulation of lactate. Further, the findings indicated that hypoxia disturbed energy metabolism and induced antioxidant defense regulation in prawns. Surprisingly, recovery from hypoxia (i.e., reoxygenation) significantly affected 25 metabolites. Some amino acids (valine, leucine, isoleucine, lysine, glutamate, and methionine) were markedly decreased compared to the control group, suggesting that increased degradation of amino acids occurred to provide energy in prawns at reoxygenation conditions. This study describes the acute metabolomic alterations that occur in prawns in response to hypoxia and demonstrates the potential of the altered metabolites as biomarkers of hypoxia.

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“…; Liu et al . ). The metabolome is so sensitive that differences in the age of fish larvae can be detected within samples that are only a few hours apart in developmental stage (Huang et al .…”

Section: Metabolomic Strategiesmentioning

confidence: 97%

“…In most cases, controls and treatment groups should have the same genetic background and should be matched for gender, age, size-class and/or development stage. For example, male and female mussels from a homogenous population can easily be discriminated based on their metabolite profiles (Cubero-Leon et al 2012), and have sex-specific physiological responses to environmental stressors, toxin exposures and pathogen infections (Ji et al 2013a,b;Ellis et al 2014;Liu et al 2014a). The metabolome is so sensitive that differences in the age of fish larvae can be detected within samples that are only a few hours apart in developmental stage (Huang et al 2013), and marine invertebrate larvae of the same age but different size-class can be discriminated based on their metabolite profiles (Young et al 2015b).…”

Section: Experimental Design and Samplingmentioning

confidence: 99%

Metabolomic strategies for aquaculture research: a primer

Young

Alfaro

2016

Reviews in Aquaculture

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Metabolomics is a fast-evolving field that provides qualitative and quantitative analyses of metabolites within cells, tissues or biofluids. Recent applications of metabolomics approaches in aquaculture research have highlighted the huge potential for solving problems within all aspects of the production line, from hatchery production to postharvest quality control. To assist with the growing application of metabolomics in aquaculture research, this contribution provides a review of techniques and steps necessary to conduct metabolomics research, from experimental design to data interpretation. Specifically, we target scientists who are new to the field of metabolomics, and we offer simple, but comprehensive steps and strategies to conduct this type of research. We conclude this primer with some advice on how to access relevant expertise and facilities for metabolomics-based aquaculture research.

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Gender-specific metabolic responses in hepatopancreas of mussel Mytilus galloprovincialis challenged by Vibrio harveyi

Cited by 32 publications

References 33 publications

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

Multifactorial Analysis of Environmental Metabolomic Data in Ecotoxicology: Wild Marine Mussel Exposed to WWTP Effluent as a Case Study

Based on the Metabolomic Approach the Energy Metabolism Responses of Oriental River Prawn Macrobrachium nipponense Hepatopancreas to Acute Hypoxia and Reoxygenation

Metabolomic strategies for aquaculture research: a primer

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