Gender-related differences in circadian rhythm of rat plasma acetyl- and butyrylcholinesterase: Effects of sex hormone withdrawal

Alves-Amaral, Gracielle; Pires-Oliveira, Marcelo; Andrade-Lopes, Ana Luiza; Chiavegatti, Tiago; Godinho, Rosely Oliveira

doi:10.1016/j.cbi.2010.04.002

Cited by 21 publications

(8 citation statements)

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“…These observations have been recapitulated in a number of animal models of AD that have demonstrated greater behavioral deficits and AD pathology in females (Oakley et al, 2006;Hirata-Fukae et al, 2008;Carroll et al, 2010). In addition, higher BChE levels have been observed in females than in males (Mundell, 1944;Illsley and Lamartiniere, 1981;Edwards and Brimijoin, 1983;Alves-Amaral et al, 2010). Furthermore, BChE levels in rat serum were observed to be three times higher in females than males, and that gonadectomy in both sexes abolished this difference.…”

Section: Discussionmentioning

confidence: 63%

Butyrylcholinesterase-knockout reduces brain deposition of fibrillar β-amyloid in an Alzheimer mouse model

2015

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In Alzheimer's disease (AD), numerous β-amyloid (Aβ) plaques are associated with butyrylcholinesterase (BChE) activity, the significance of which is unclear. A mouse model, containing five human familial AD genes (5XFAD), also develops Aβ plaques with BChE activity. Knock-out of BChE in this model showed diminished fibrillar Aβ plaque deposition, more so in males than females. This suggests that lack of BChE reduces deposition of fibrillar Aβ in AD and this effect may be influenced by sex.

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Section: Discussionmentioning

confidence: 63%

Butyrylcholinesterase-knockout reduces brain deposition of fibrillar β-amyloid in an Alzheimer mouse model

2015

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“…como la genética, la edad, el género, el estado reproductivo, la regulación endocrina e incluso los ritmos circadianos y épocas climáticas, el estado nutricional y de salud de los organismos, la temperatura o la presencia en el medio de metales o biotoxinas (Roberts et al 1988, Alves-Amaral et al 2010. Por ello, la evaluación de la AChE como biomarcador específico para la exposición a plaguicidas OP debe complementarse con otros biomarcadores de exposición que puedan dar más información sobre los riesgos que representa los plaguicidas OP.…”

Section: Tejidounclassified

Organochlorine and anticholinergic pesticides in wild mice from wetland ecosystems of the Gulf of Mexico

Chi-Coyoc¹,

Escalona‐Segura²,

Moncada³

et al. 2016

Therya

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Terminos Lagoon area is a highly important economic and biological area, and one of the most important fresh water reservoirs in Mesoamerica. This area has been affected by human activities such as the disposal of pollutants, including pesticides that cause damage among wildlife. Our research evaluated the exposition of three wild rodent species Oryzomys couesi, Peromyscus leucopus, and Reithrodontomys gracilis to organochlorine and anticholinergic pesticides in nine localities of Terminos Lagoon. We collected samples of brain, skeletal muscle, and liver to evaluate organochlorine and anticholinergic pesticides exposure. We measure acetylcholinesterase (AChE) activity as anticholinergic pesticide exposure sign. We found significant differences in acetylcholinesterase activity among localities in brain for two species O. couesi and R. gracilis, but not for skeletal muscle tissue. We found 20 organochlorine compounds in rodent´s liver of which only lindane, aldrine and heptachlor were in amounts suggesting a recent exposure. The AChE inhibition is not enough evidence for anticholinergic pesticide exposure. Recent organochlorine pesticides application and exposition was detected, but sources are unknown, probably atmospheric deposition. Rodent exposure to pesticides could affect negatively its population dynamic. This detrimental effects can reach higher trophic levels because predators biomagnifies some of these compounds.Key words: Aldrin; AChE inhibition; carbamates; DDT; organophosphorus; Pollution; wild rodents.El área de Laguna de Términos es de gran importancia económica y biológica y es uno de los reservorios de agua dulce más importantes de Mesoamérica, la cual ha sido impactada por las actividades antrópicas que incorporan contaminantes, entre ellos los plaguicidas, los cuales dañan a la fauna silvestre. Este trabajo evaluó la exposición de tres especies de ratones silvestres Oryzomys couesi, Peromyscus leucopus y Reithrodontomys gracilis a plaguicidas anticolinérgicos y organoclorados en nueve sitios de Laguna de Términos. Se colectaron muestras de cerebro, músculo e hígado. Se midió la actividad de la enzima acetilcolinesterasa en músculo y cerebro como evidencia de la exposición a plaguicidas anticolinérgicos. Se determinaron lo compuestos organoclorados y sus concentraciones en muestras de hígado como evidencia de la exposición a los mismos. Se encontraron diferencias en la actividad de la enzima acetilcolinesterasa entre zonas de muestreo en cerebro para O. couesi y R. gracilis pero no en músculo. Se detectaron 20 compuestos organoclorados en el hígado de los ratones, de los cuales sólo el lindano, el aldrín y el heptacloro se encontraron en cantidades que sugieren una exposición reciente. La inhibición del a actividad de la AChE no es evidencia contundente para la exposición a plaguicidas anticolinérgicos en el área. Se detectó la aplicación y exposición reciente a plaguicidas OC, el pueden ser las deposiciones atmosféricas. La exposición de los ratones a los plaguicidas puede afectar negativamente ...

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“…After centrifugation (3,000 g, 4°C) for 20 min, plasma was collected and kept at Ϫ20°C until the determination of cholinesterase activity. Enzymatic assays were performed using an adaptation of the colorimetric method (24), as described by Alves-Amaral et al (1). Plasma samples (10 l) were incubated in 96-well microplates with 0.01 M 5,5=-dithio-bis-(2-nitrobenzoic acid, DTNB, Sigma-Aldrich) and the excess of the substrate (acetylthiocholine, 0.075 M, SigmaAldrich) in 0.2 mM phosphate buffer, pH 8.0 at 30°C, in the presence of the selective inhibitor of butyrylcholinesterase, tetraisopropylpyrophosphoramide (IsoOMPA 10 Ϫ3 M, Sigma-Aldrich).…”

mentioning

confidence: 99%

Increase in parasympathetic tone by pyridostigmine prevents ventricular dysfunction during the onset of heart failure

Lataro¹,

Silva²,

Fazan³

et al. 2013

American Journal of Physiology-Regulatory, Integrative and Comp

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Lataro RM, Silva CA, Fazan R Jr, Rossi MA, Prado CM, Godinho RO, Salgado HC. Increase in parasympathetic tone by pyridostigmine prevents ventricular dysfunction during the onset of heart failure. Am J Physiol Regul Integr Comp Physiol 305: R908 -R916, 2013. First published August 28, 2013 doi:10.1152/ajpregu.00102.2013.-Heart failure (HF) is characterized by elevated sympathetic activity and reduced parasympathetic control of the heart. Experimental evidence suggests that the increase in parasympathetic function can be a therapeutic alternative to slow HF evolution. The parasympathetic neurotransmission can be improved by acetylcholinesterase inhibition. We investigated the long-term (4 wk) effects of the acetylcholinesterase inhibitor pyridostigmine on sympathovagal balance, cardiac remodeling, and cardiac function in the onset of HF following myocardial infarction. Myocardial infarction was elicited in adult male Wistar rats. After 4 wk of pyridostigmine administration, per os, methylatropine and propranolol were used to evaluate the cardiac sympathovagal balance. The tachycardic response caused by methylatropine was considered to be the vagal tone, whereas the bradycardic response caused by propranolol was considered to be the sympathetic tone. In conscious HF rats, pyridostigmine reduced the basal heart rate, increased vagal, and reduced sympathetic control of heart rate. Pyridostigmine reduced the myocyte diameter and collagen density of the surviving left ventricle. Pyridostigmine also increased vascular endothelial growth factor protein in the left ventricle, suggesting myocardial angiogenesis. Cardiac function was assessed by means of the pressure-volume conductance catheter system. HF rats treated with pyridostigmine exhibited a higher stroke volume, ejection fraction, cardiac output, and contractility of the left ventricle. It was demonstrated that the long-term administration of pyridostigmine started right after coronary artery ligation augmented cardiac vagal and reduced sympathetic tone, attenuating cardiac remodeling and left ventricular dysfunction during the progression of HF in rats.

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Gender-related differences in circadian rhythm of rat plasma acetyl- and butyrylcholinesterase: Effects of sex hormone withdrawal

Cited by 21 publications

References 57 publications

Butyrylcholinesterase-knockout reduces brain deposition of fibrillar β-amyloid in an Alzheimer mouse model

Butyrylcholinesterase-knockout reduces brain deposition of fibrillar β-amyloid in an Alzheimer mouse model

Organochlorine and anticholinergic pesticides in wild mice from wetland ecosystems of the Gulf of Mexico

Increase in parasympathetic tone by pyridostigmine prevents ventricular dysfunction during the onset of heart failure

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