Gender-related differences in basal DNA damage in lymphocytes of a healthy Indian population using the alkaline Comet assay

Bajpayee, Mahima; Dhawan, Alok; Parmar, Devendra; Pandey, Alok Kumar; Mathur, Neeraj; Seth, Prahlad K.

doi:10.1016/s1383-5718(02)00175-4

Cited by 103 publications

(54 citation statements)

References 34 publications

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“…Several studies have observed-even in young, healthy individuals-higher levels of DNA damage in men than women (24) in agreement with greater levels of other oxidative markers in peripheral blood cells and in skeletal muscle biopsies (25). Oxidative DNA damage, together with a greater production of oxidant species and lower antioxidant properties, parallels the degree of insulin resistance (7) and progression of the metabolic syndrome in humans (4).…”

Section: Discussionmentioning

confidence: 70%

Maternal and Sex Dependency of Insulin Resistance: Longitudinal PET and Echocardiography Study from the Healthy Fetus to the Adult Minipig

et al. 2011

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Cardiovascular and metabolic vulnerability have an early developmental origin. We evaluated the potential influence of innate life factors, including the metabolism of the mother and the sex of the offspring, on cardiometabolic risk, including organ-specific insulin resistance, subclinical cardiac dysfunction, and DNA oxidative damage throughout the lifespan. Methods: Two female minipigs were studied during late pregnancy, and their offspring were restudied at the ages of 1 mo (n 5 11), 6 mo (n 5 9), and 9 mo (n 5 10, 6 offspring and 4 age-matched animals). We measured insulin-mediated glucose disposal in skeletal muscle, adipose tissue, liver, and myocardium using 18 F-FDG PET; cardiac function using 2-dimensional strain echocardiography; and DNA damage using the comet assay. Results: Glucose metabolism showed the 2 sows to have differences similar to those in their respective 1-mo-old offspring. Over time, compared with female animals, male animals developed myocardial insulin resistance (male animals vs. female animals: 34 6 5 vs. 58 6 8 mmol/min/kg at 6 mo, P 5 0.03; 29 6 8 vs. 60 6 7 mmol/min/kg at 9 mo, P 5 0.02). Cardiac function progressively deteriorated in male animals from 1 mo (radial strain, 260% 6 7%; strain rate, 25.4 6 0.9 s 21 ) to 6 mo (radial strain, 241% 6 5%; strain rate, 22.5 6 0.2 s 21 , P , 0.05 vs. 1 mo) and 9 mo (radial strain, 232% 6 5%; strain rate, 21.6 6 0.2 s 21 , P , 0.01 vs. 1 mo) and was significantly different from that in female animals (radial strain, 248% 6 4%; strain rate, 23.1 6 0.2 s 21 , P , 0.05 and P , 0.01, respectively). Oxidative damage was reduced in female animals and increased in male animals across age categories (P , 0.05). Conclusion: The metabolism of minipig offspring is influenced by maternal insulin sensitivity during early life stages. Sex-related effects prevail thereafter in healthy minipigs, documenting a precocious onset of cardiometabolic vulnerability in male offspring.

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Section: Discussionmentioning

confidence: 70%

Maternal and Sex Dependency of Insulin Resistance: Longitudinal PET and Echocardiography Study from the Healthy Fetus to the Adult Minipig

et al. 2011

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“…Some biomonitoring studies found positive association of age (Ozkul et al,1997;Moretti et al, 2000), sex (Betti et al, 1994;Bajpayee et al, 2002;Fenech et al, 2003), smoking (Burgaz et al, 1999;Hoffmann et al, 2005), alcohol intake (Ramirez et al, 2002) and tobacco chewing (Nair et al, 1991;Trivedi et al, 1993) while some studies observed no association of age (Zhu et al, 1999;Konapaka et al, 2003), sex (Anderson et al, 1993;Zhu et al, 1999;Konapaka et al, 2003), smoking (Moller et al, 2006), alcohol intake (Celik et al, 2007;Martinez-Valenzuela et al, 2009) with genetic damage. In our study, we did not observe any significant association of age, sex and consumption habits (smoking and alcohol use) with genetic damage.…”

Section: Discussionmentioning

confidence: 99%

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Giri

Yadav²,

Kumar

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Aim: It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coaltar workers exposed to PAHs at their work place. Methods: Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects. Results: Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects. Conclusion: In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt)+homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.

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“…Comet assay was carried out in A549 and IM9 cells to study DNA damage using the method of Bajpayee 29 , et al with some modifications. Slides were prepared in duplicate and lysed overnight in lysing solution (pH 10.0).…”

Section: Dna Damagementioning

confidence: 99%

Ultrafine Particles of Diesel Exhaust Induces Cytochrome P450 1A1 Mediated Oxidative Stress and DNA Damage in Cultured Blood and Lung Cells

et al. 2016

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Attempts were made to investigate the role of cytochrome P450 1A1 (CYP1A1) on similarities in the generation of reactive oxygen species (ROS) and DNA damage in IM9, a human B lymphoblastic cell line with A549, the human lung adenocarcinoma epithelial cell line on exposure of diesel exhaust particles (DEP). A suspension of ultrafine particles (< 0.2 µM) of DEP (1mg/ml) in DMEM-F12 medium, at a concentration range of 1-100 µg/ml, was added to the cells for 6-48h. Expression studies revealed that DEP induced similar increase in the expression of CYP1A1, generation of ROS and DNA damage in both the cells. Pre-incubation with 3-methylcholanthrene (MC), a CYP1A1 inducer resulted in higher magnitude of induction of CYP1A1, ROS and DNA damage. This synergistic effect was lowered when α-naphthoflavone (α-NF), an inhibitor of CYP1A1 catalysed reactions, was added to these cells. Though the magnitude of alterations was lower in IM9 cells when compared to A549 cells, similarities in the alterations in blood and lungs cells has further suggested that blood lymphocytes can be used as a surrogate to monitor toxicity of vehicular emissions.

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Gender-related differences in basal DNA damage in lymphocytes of a healthy Indian population using the alkaline Comet assay

Cited by 103 publications

References 34 publications

Maternal and Sex Dependency of Insulin Resistance: Longitudinal PET and Echocardiography Study from the Healthy Fetus to the Adult Minipig

Maternal and Sex Dependency of Insulin Resistance: Longitudinal PET and Echocardiography Study from the Healthy Fetus to the Adult Minipig

CYP1A1 Gene Polymorphisms: Modulator of Genetic Damage in Coal-Tar Workers

Ultrafine Particles of Diesel Exhaust Induces Cytochrome P450 1A1 Mediated Oxidative Stress and DNA Damage in Cultured Blood and Lung Cells

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