Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days

Qi, Baiyu; Kong, Ling-Zhu; Lu, Yingqiang

doi:10.1016/j.jflm.2012.09.014

Cited by 15 publications

(10 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative mRNA / rRNA expression versus the age of the stain followed a linear trend for up to 60 days. Besides, Qi et al examined the β-actin / 18S-rRNA ratio in blood, aged for 28 days, from 16 Han Chinese [32]. The ratio of the two RNA species changed in a linear fashion but a gender-effect was observed.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

See 1 more Smart Citation

Degradation of human mRNA transcripts over time as an indicator of the time since deposition (TsD) in biological crime scene traces

Salzmann

Russo

Kreutzer

et al. 2021

Forensic Science International: Genetics

View full text Add to dashboard Cite

show abstract

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

“…For 18s-J o u r n a l P r e -p r o o f rRNA conflicting results were reported. Some studies identified 18s-RNA to be relatively stable [30,32,33], while others reported a degrading pattern [31,34,35,54].…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

Degradation of human mRNA transcripts over time as an indicator of the time since deposition (TsD) in biological crime scene traces

Salzmann

Russo

Kreutzer

et al. 2021

Forensic Science International: Genetics

View full text Add to dashboard Cite

show abstract

“…Atomic force microscopy has investigated the changes in RBC physiology over time [51][52][53] while mass-spectrometry 54,55 , chromatography [56][57][58][59][60] and fluorescence spectroscopy [61][62][63] have measured changes in metabolites and protein composition of deposited bloodstains. DNA 38,39,[64][65][66][67] , total RNA, mRNA and miRNA [10][11][12][68][69][70][71][72][73][74][75][76][77][78][79][80] degradation have also been targeted in longer time series. Recently, microbial profiling of bloodstains has described the timewise changes in microbial communities inhabiting the bloodstains 81,82 .…”

Section: Introductionmentioning

confidence: 99%

The crux of time: A meta-analysis of ex vivo whole blood degradation

Elliott

Shafer

Stotesbury

2022

Preprint

View full text Add to dashboard Cite

Estimating the time since deposition (TSD) of a bloodstain can provide important medico-legal information for crime scene investigation. Research in this area primarily investigates the degradation of either hemoglobin or genetic material over time. In this work, we present a comprehensive meta-analysis on bloodstain TSD research. Our results are interpreted from 25 quantitative studies used to probe the effect of biomolecule studied, analytical technique used, substrate porosity, environmental conditions, and blood source on TSD estimates. There was an overall strong effect of time across studies (Fisher’s Zr = 1.66, r = 0.93), and generally, we found that the type of biomolecule studied (e.g., hemoglobin, DNA) had equal effect sizes for TSD estimation. Differences in the mean TSD effect size were also observed between substrate porosity. Interestingly, the blood source does not significantly influence the magnitude of the effect sizes in TSD estimation. Despite the clear effect of time, forensically relevant prediction of bloodstain TSD remains complicated by inter-donor variability, type of substrate and environmental conditions. We recommend that future bloodstain TSD research increase sample size, include summary statistics and standardize experimental methodologies so that we can develop a quantitative understanding of the physicochemical processes involved in whole blood degradation in ex vivo conditions.

show abstract

“…1,2 There has been a recent tendency to analyse biochemical parameters from dried blood, with the assumption that this method provides convenience in terms of the storage and shipping of blood samples. 3,4 In medicolegal cases, the identification of chronic and metabolic diseases of those involved by studying biochemical markers in bloodstains recovered from a crime scene can be an important step in elucidating the case.…”

Section: Introductionmentioning

confidence: 99%

Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

et al. 2019

View full text Add to dashboard Cite

This study aimed to determine haemoglobin A1c (HbA1c) levels in bloodstains shed on glass and fabric surfaces on specified test dates. Blood samples were taken from 26 patients (13 diabetic and 13 non-diabetic). Initial HbA1c levels were detected by using high-performance liquid chromatography (HPLC), and bloodstains were created on both cotton fabric and glass surfaces. Samples were processed at different ages (0, 7, 14, 28 and 56 days) by diluting distilled water and then measuring HbA1c levels by HPLC again. In all stains, HbA1c levels could be determined by using HPLC, but there was a moderate rise in accordance with the age of the stains. A statistically significant difference was found for bloodstains on clothes compared to those on glass surfaces. Receiver operating curve analysis found a sensitivity of 1.0 and specificity of 0.923 (cut-off 6.55) for glass surfaces on the seventh day; a sensitivity of 1.0, a specificity of 0.846 (cut-off 6.45) for clothes on the seventh day; a sensitivity of 1.0 and a specificity of 0.923 (cut-off 6.85) for clothes on the 56th day; and a sensitivity of 1.0 and a specificity of 0.846 (cut-off 7.55) for glass surfaces on the 56th day. In conclusion, this study found that HbA1c levels could be measured with high reliability from forensic bloodstains by using HPLC. Thus, in cases where DNA data banks cannot identify individuals, it would make sense to turn to those who have a medical history of diabetes among the suspects with the results of high HbA1c levels.

show abstract

Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days

Cited by 15 publications

References 23 publications

Degradation of human mRNA transcripts over time as an indicator of the time since deposition (TsD) in biological crime scene traces

Degradation of human mRNA transcripts over time as an indicator of the time since deposition (TsD) in biological crime scene traces

The crux of time: A meta-analysis of ex vivo whole blood degradation

Determination of haemoglobin A1c levels using high-performance liquid chromatography of bloodstains

Contact Info

Product

Resources

About