Gender modulation of Ca2+ uptake in cardiac mitochondria

Arieli, Yehuda; Gursahani, H.; Eaton, Matt; Hernandez, Lisa A.; Schaefer, Saul

doi:10.1016/j.yjmcc.2004.04.023

Cited by 60 publications

(57 citation statements)

References 28 publications

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“…This value is between the reported fluxes of Ca +2 measured in isolated mitochondria for physiologic conditions (32 to 350 nmol Ca 2+ /g/s). 25,26 Moreover, the present results show that 5-hydroxydecanoate was able to prevent the postischemic stunning previously seen in CPG hearts pretreated with 10 mmol/L Clzp, 17 suggesting opposite effects of both drugs. This was confirmed by the reappearance of stunning when Clzp was increased to 30 mmol/L under CPG + 100 mmol/L 5-hydroxydecanoate ( Fig.…”

Section: Discussionsupporting

confidence: 61%

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

Ragone

Consolini

2009

Journal of Cardiovascular Pharmacology

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Section: Discussionsupporting

confidence: 61%

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

Ragone

Consolini

2009

Journal of Cardiovascular Pharmacology

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“…chondria (0.3 mg/ml) were incubated in 2 ml of buffer D, and redox state of PNs was measured in the presence of glutamate-malate (5 mM-2.5 mM) or succinate (5 mM) Ϯ rotenone (1 M). Redox state of PN was calculated as described by Arieli et al (2): NAD(P)H ratio ϭ (F s Ϫ Fmin)/(Fmax Ϫ Fmin), where Fs is the fluorescence measured after addition of respiratory substrates, Fmin is the baseline fluorescence of mitochondria measured in the absence of respiratory substrates, which represents PNs in their fully oxidized form, and F max is the fluorescence recorded after addition of 2 M antimycin A, which represents PNs in their fully reduced form. Preliminary experiments indicated that F min values measured with this method were similar to those obtained when mitochondria were uncoupled by the addition of 100 nM CCCP.…”

Section: Methodsmentioning

confidence: 99%

Exercise training induces respiratory substrate-specific decrease in Ca²⁺-induced permeability transition pore opening in heart mitochondria

Marcil

Bourduas²,

Ascah³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

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The purpose of this study was to determine whether regular exercise (treadmill running, 10 wk) alters the susceptibility of rat isolated heart mitochondria to Ca(2+)-induced permeability transition pore (PTP) opening and whether this could be associated with changes in the modulation of PTP opening by selected physiological effectors. Basal leak-driven and ADP-stimulated respiration in the presence of substrates for complex I, II, and IV were not affected by training. Fluorimetric studies revealed that in the control and exercise-trained groups, the amount of Ca(2+) required to trigger PTP opening was greater in the presence of complex II vs. I substrates (230 +/- 12 vs. 134 +/- 7 nmol Ca(2+)/mg protein, P < 0.01; pooled average of control and trained groups). In addition, with a substrate feeding the complex II, training increased by 45% (P < 0.01) the amount of Ca(2+) required to trigger PTP opening both in the presence and absence of the PTP inhibitor cyclosporin A. However, membrane potential, reactive oxygen species production, NAD(P)H ratio, and Ca(2+) uptake kinetics were not different in mitochondria from both groups. Together, these results suggest the existence of a substrate-specific regulation of the PTP in heart mitochondria and suggest that regular exercise results in a reduced sensitivity to Ca(2+)-induced PTP opening in presence of complex II substrates.

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“…However, similar to the mammalian heart, the trout heart is hypoxia-intolerant and experiences pronounced decrements in contractile function (2,24,33). During these periods, oxidation of fatty acids will be limited and ATP production is likely to be supported by anaerobic glycolysis.…”

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confidence: 99%

Sex differences in energy metabolism and performance of teleost cardiac tissue

Battiprolu¹,

Harmon²,

Rodnick³

2007

American Journal of Physiology-Regulatory, Integrative and Comp

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This study examined the effects of different oxygenation levels and substrate availability on cardiac performance, metabolism, and biochemistry in sexually immature male and female rainbow trout (Oncorhynchus mykiss). Ventricle strips were electrically paced (0.5 Hz, 14 degrees C) in hyperoxic or hypoxic Ringer solution. Our results demonstrate that 1) males sustain isometric force production (F) longer than females under hyperoxia (P O2 = 640 mmHg) with exogenous glucose present; 2) contractility is not maintained under moderate (P O2 = 130 mmHg) or severe hypoxia (P O2 = 10-20 mmHg) with glucose in either sex; however, following reoxygenation, F is higher in females compared with males; and 3) female tissue has higher lactate levels, net lactate efflux, and lactate dehydrogenase activity than males, whereas males have higher glycogen, citrate synthase, and beta-hydroxy acyl-CoA dehydrogenase activities, and greater inotropic responses to exogenous glucose and octanoate. No sex differences were detected in responsiveness to epinephrine and inhibitors of glucose transport or activities of hexokinase and pyruvate kinase. We conclude that sex differences exist in rainbow trout cardiac tissue: females appear to prefer glycolysis for ATP production, whereas males have a higher capacity for aerobic and lipid metabolism.

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Gender modulation of Ca2+ uptake in cardiac mitochondria

Cited by 60 publications

References 28 publications

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

Exercise training induces respiratory substrate-specific decrease in Ca²⁺-induced permeability transition pore opening in heart mitochondria

Sex differences in energy metabolism and performance of teleost cardiac tissue

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Gender modulation of Ca2+ uptake in cardiac mitochondria

Cited by 60 publications

References 28 publications

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

CARDIAC Role of the Mitochondrial Ca2+ Transporters in the High-[K+]o Cardioprotection of Rat Hearts Under Ischemia and Reperfusion: A Mechano-Energetic Study

Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore opening in heart mitochondria

Sex differences in energy metabolism and performance of teleost cardiac tissue

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Exercise training induces respiratory substrate-specific decrease in Ca²⁺-induced permeability transition pore opening in heart mitochondria