Gender-enriched transcription of activation associated secreted proteins in Ostertagia ostertagi

Visser, Aline; Zeveren, A.M. Van; Meyvis, Yves; Peelaers, Iris; Broeck, Wim Van Den; Gevaert, Kris; Vercruysse, J.; Claerebout, Edwin; Geldhof, Peter

doi:10.1016/j.ijpara.2007.08.008

Cited by 24 publications

(18 citation statements)

References 38 publications

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“…Adult ES proteins in O. ostertagi identified a homologue of an ASP and a vitellogenin [92], which were not identified in T. circumcincta ES proteins [101]. However, we have successfully identified a globin-like protein and Na-ASP-2 - a PR-1 protein from Necator americanus ) [102] and ASP-3 from O. ostertagi [103]. ASPs are the members of a group of nematode-specific molecules [5].…”

Section: Discussionmentioning

confidence: 99%

An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications

et al. 2012

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BackgroundTeladorsagia circumcincta (order Strongylida) is an economically important parasitic nematode of small ruminants (including sheep and goats) in temperate climatic regions of the world. Improved insights into the molecular biology of this parasite could underpin alternative methods required to control this and related parasites, in order to circumvent major problems associated with anthelmintic resistance. The aims of the present study were to define the transcriptome of the adult stage of T. circumcincta and to infer the main pathways linked to molecules known to be expressed in this nematode. Since sheep develop acquired immunity against T. circumcincta, there is some potential for the development of a vaccine against this parasite. Hence, we infer excretory/secretory molecules for T. circumcincta as possible immunogens and vaccine candidates.ResultsA total of 407,357 ESTs were assembled yielding 39,852 putative gene sequences. Conceptual translation predicted 24,013 proteins, which were then subjected to detailed annotation which included pathway mapping of predicted proteins (including 112 excreted/secreted [ES] and 226 transmembrane peptides), domain analysis and GO annotation was carried out using InterProScan along with BLAST2GO. Further analysis was carried out for secretory signal peptides using SignalP and non-classical sec pathway using SecretomeP tools.For ES proteins, key pathways, including Fc epsilon RI, T cell receptor, and chemokine signalling as well as leukocyte transendothelial migration were inferred to be linked to immune responses, along with other pathways related to neurodegenerative diseases and infectious diseases, which warrant detailed future studies. KAAS could identify new and updated pathways like phagosome and protein processing in endoplasmic reticulum. Domain analysis for the assembled dataset revealed families of serine, cysteine and proteinase inhibitors which might represent targets for parasite intervention. InterProScan could identify GO terms pertaining to the extracellular region. Some of the important domain families identified included the SCP-like extracellular proteins which belong to the pathogenesis-related proteins (PRPs) superfamily along with C-type lectin, saposin-like proteins. The 'extracellular region' that corresponds to allergen V5/Tpx-1 related, considered important in parasite-host interactions, was also identified.Six cysteine motif (SXC1) proteins, transthyretin proteins, C-type lectins, activation-associated secreted proteins (ASPs), which could represent potential candidates for developing novel anthelmintics or vaccines were few other important findings. Of these, SXC1, protein kinase domain-containing protein, trypsin family protein, trypsin-like protease family member (TRY-1), putative major allergen and putative lipid binding protein were identified which have not been reported in the published T. circumcincta proteomics analysis.Detailed analysis of 6,058 raw EST sequences from dbEST revealed 315 putatively secreted proteins. Amongst the...

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Section: Discussionmentioning

confidence: 99%

An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications

et al. 2012

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“…VAL-1 also has significant sequence homology with venom allergens from vespid wasps (Ag5) and fire ants (Ag3). 30 A study by Visser et al 32 showed that VAL-1 and its homolog may have a role in the reproductive development of the male Ostertagia worms. Thus the VAL-1 homolog appears to be important for the establishment and survival of the parasite in the host 20 and may have a possible role in immune evasion.…”

Section: Discussionmentioning

confidence: 99%

Multivalent vaccine formulation with BmVAL-1 and BmALT-2 confer significant protection against challenge infections with Brugia malayi in mice and jirds

Kalyanasundaram

Balumuri²

2011

RRTM

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Purpose Lymphatic filariasis, a mosquito-borne infection, affects 120 million people in 83 different countries. Mass drug administration is fully underway in several parts of the world to eradicate this infection by year 2020. Drugs alone are highly efficient treatments, but long-term sustainable prophylaxis requires an effective vaccine. No vaccines are available for humans and animals despite several potential candidate vaccine antigens having been identified. Brugia malayi vespid venom allergen homolog-like protein (BmVAL-1) and B. malayi abundant larval transcript (BmALT-2) are two of the most promising vaccine candidates. We evaluated various vaccination regimens consisting of DNA and protein antigens and evaluated the potential of monovalent and multivalent vaccine formulations in mice and jird animal models. Methods Mice and jirds were vaccinated with monovalent DNA preparations of BmVAL-1 or BmALT-2 in pVAX-1 vector or monovalent protein preparations of rBmVAL-1 and rBmALT-2 in alum using a homologous or heterologous prime boost approach. These vaccine regimens were then compared with a multivalent vaccine formulation consisting of DNA or hybrid protein formulation of the two antigens. Challenge experiments were performed with B. malayi L3 in mice and jirds to evaluate the degree of protection, and immunological parameters were determined in mice and humans to elucidate the characteristics of the protective immune responses. Results Vaccination with monovalent BmVAL-1 vaccine conferred 39% (DNA vaccine) to 54% (DNA prime and protein boost) protection in mice. A similar degree of protection was observed in jirds (50% to 52%). Monovalent BmALT-2 afforded 51% to 75% protection in mice and 58% to 79% protection in jirds. Our testing of a multivalent formulation of BmVAL-1 and BmALT-2, showed 57% to 82% protection in mice and 77% to 85% protection in jirds. A heterologous prime boost approach using the multivalent vaccine gave the highest degree of protection in both mice and jirds. Serological analysis in mice showed that BmVAL-1 vaccination induced an IgG1, IgG2a, and IgG3 antibody response, whereas BmALT-2 vaccination predominantly induced an IgG1 and IgG3 antibody response. Cytokine responses of antigen-responding cells in the spleen secreted predominantly IFN-γ and IL-5 in response to BmVAL-1, and IL-4, and IL-5 in response to BmALT-2. Conclusion A multivalent vaccine formulation of BmVAL-1 and BmALT-2 given as a prime boost regimen gave significant protection against lymphatic filariasis caused by B. malayi in mice and jirds. Because putatively immune endemic normal subjects also carry protective antibodies against BmVAL-1 and BmALT-2, developing this multivalent formulation as a prophylactic vaccine against B. malayi for human and veterinary use has great potential.

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“…SCP/TAPS belong to a large group of proteins that include the Ancylostoma -secreted proteins (ASPs; Cantacessi et al, 2009b). In parasitic nematodes, ASPs were first characterized for hookworms (Hawdon et al, 1996, 1999) and subsequently from related strongylid nematodes (Visser et al, 2008). As ASPs are abundant in the excretory/secretory (ES) products of the infective L3, they are thought to play an important role in the transition from the free-living to the parasitic stage of a nematode during its invasion of the host (Hawdon et al, 1996, 1999; Moser et al, 2005; Datu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

First transcriptomic analysis of the economically important parasitic nematode, Trichostrongylus colubriformis, using a next-generation sequencing approach

Cantacessi

Mitreva

Campbell

et al. 2010

Infection, Genetics and Evolution

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Trichostrongylus colubriformis (Strongylida), a small intestinal nematode of small ruminants, is a major cause of production and economic losses in many countries. The aims of the present study were to define the transcriptome of the adult stage of T. colubriformis, using 454 sequencing technology and bioinformatic analyses, and to predict the main pathways that key groups of molecules are linked to in this nematode. A total of 21,259 contigs were assembled from the sequence data produced from a normalized cDNA library; 7,876 of these contigs had known orthologues in the free-living nematode Caenorhabditis elegans, and encoded, amongst others, proteins with ‘transthyretin-like’ (8.8%), ‘RNA recognition’ (8.4%) and ‘metridin-like ShK toxin’ (7.6%) motifs. Bioinformatic analyses inferred that relatively high proportions of the C. elegans homologues are involved in biological pathways linked to ‘peptidases’ (4%), ‘ribosome’ (3.6%) and ‘oxidative phosphorylation’ (3%). Highly represented were peptides predicted to be associated with the nervous system, digestion of host proteins or inhibition of host proteases. Probabilistic functional gene networking of the complement of C. elegans orthologues (n = 2,126) assigned significance to particular subsets of molecules, such as protein kinases and serine/threonine phosphatases. The present study represents the first, comprehensive insight into the transcriptome of adult T. colubriformis, which provides a foundation for fundamental studies of the molecular biology and biochemistry of this parasitic nematode as well as prospects for identifying targets for novel nematocides. Future investigations should focus on comparing the transcriptomes of different developmental stages, both genders and various tissues of this parasitic nematode for the prediction of essential genes/gene products that are specific to nematodes.

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Gender-enriched transcription of activation associated secreted proteins in Ostertagia ostertagi

Cited by 24 publications

References 38 publications

An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications

An analysis of the transcriptome of Teladorsagia circumcincta: its biological and biotechnological implications

Multivalent vaccine formulation with BmVAL-1 and BmALT-2 confer significant protection against challenge infections with Brugia malayi in mice and jirds

First transcriptomic analysis of the economically important parasitic nematode, Trichostrongylus colubriformis, using a next-generation sequencing approach

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