Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats

Choi, Jung-Won; Aseer, Kanikkai Raja; Chaudhari, Harmesh N.; Mukherjee, Rajib; Choi, Minsu; Yun, Jong Won

doi:10.1002/pmic.201200529

Cited by 11 publications

(8 citation statements)

References 65 publications

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“…Data represent mean ± SEM, n = 5 in each group. a p \ 0.05 as compared to the aFC group with the data of Choi et al [23] who did not observe gender-specific differences in controls. This difference may be ascribed to strain-specific variations, as in our study, Wistar rats were used and in the study of Choi et al [23] Sprague-Dawley rats were investigated.…”

Section: Discussionmentioning

confidence: 65%

“…a p \ 0.05 as compared to the aFC group with the data of Choi et al [23] who did not observe gender-specific differences in controls. This difference may be ascribed to strain-specific variations, as in our study, Wistar rats were used and in the study of Choi et al [23] Sprague-Dawley rats were investigated. However, the higher content of glucose in control male Wistar rats, as a model, seems to be from this point of view nearer to human population as shown by slightly higher glucose level in men as compared to women without any history of diabetes [24].…”

Section: Discussionmentioning

confidence: 65%

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Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex

et al. 2015

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Time sequence study was performed to characterize the effects of diabetes mellitus type 1 on properties of the Na, K-ATPase in cerebral cortex derived from normal and streptozotocin (STZ)-diabetic rats of both genders. The samples were excised at varying time intervals of diabetes induced by STZ (65 mg kg(-1)) for 8 days, and 8 and 16 weeks. Expression of α1-3 isoforms of Na, K-ATPase was not altered in statistically significant level during all stages of diabetes neither in female nor in male rats as revealed from Western blot analysis. Studies of kinetic properties of the enzyme resulted in variations in active number of Na, K-ATPase molecules as well as its qualitative properties. Sixteen-week-old control male rats showed better affinity to substrate as indicated by 13 % decrease of K m value. The effect persisted also in males subjected to 8 days lasting diabetes; however, in males subjected to 8 weeks lasting diabetes, the effect was lost. In 25-week-old rats, the Na, K-ATPase revealed again altered properties in males and females but the mechanism of the variation was different. In females, the number of active molecules of Na, K-ATPase was higher by 32 % in controls and by 17 % in rats with chronic diabetes when comparing to respective male groups as suggested by increased value of V max. So the properties of Na, K-ATPase in cerebral cortex, playing crucial role in maintaining intracellular homeostasis of Na(+) ions, depend on gender, age, and duration of diabetic insult.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 65%

Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex

et al. 2015

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“…In our earlier study, we found that plasma levels of both Ft-A and Ft-B are significantly lower in males after HFD feeding while remarkably higher in females [27]. Moreover, Ft-B has been shown to be up-regulated in plasma of both male and female diabetic rats [28]. Those results led us to hypothesize that gender-dimorphic regulation of fetuins is associated with hormonal effects.…”

Section: Introductionmentioning

confidence: 98%

Sex Hormones Regulate Hepatic Fetuin Expression in Male and Female Rats

Kim

Choi

Lee

et al. 2014

Cell Physiol Biochem

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Background: To date, there are limited studies on the sex-specific relationship between fetuins (Ft-A and Ft-B) and metabolic diseases. Our recent proteomic study has shown that fetuins may play sex-dependent roles in obesity and diabetes. In the present study, we investigated the expression of hepatic fetuins with respect to the effects of sex hormones both in vivo and in vitro. Methods & Results: A sex hormone-treated rat model was established in order to study the effects of sex hormones on hepatic fetuin expression. Animal experiments revealed that 17β-estradiol (E2)- and dihydrotestosterone (DHT)-treated rats showed opposite effects in terms of body weight gain in both genders. Interestingly, Ft-A and Ft-B were sex-dependently expressed in the livers of rats, responding to different regulatory modes of sex hormone receptors (ERα, ERβ, and AR). To validate in vivo data, rat normal liver cells were treated with E2 or DHT at different concentrations, and similar expression patterns as those in the animal-based experiments were confirmed. We found that these changes were mediated via sex hormone receptors using antagonist experiments. Conclusion: The results of the present study indicate that sex hormones induce gender-dimorphic expression of hepatic fetuins directly via sex hormone receptors. To the best of our knowledge, this is the first approach to address the effects of sex hormones on hepatic fetuin expression.

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“…Rat is one of the most commonly used experimental animal and has been utilized as a model organism in almost every biomedical research for human diseases. Many of these studies involved the analysis of plasma proteins since as blood constantly circulate through the body and perfuse the tissues and organs, the protein changes would directly reflect the physiological and pathological status of the animal . The complexity of plasma proteins, however, sets out analytical challenges and generally requires high‐resolution separation and sensitive detection.…”

Section: Introductionmentioning

confidence: 99%

“…In shotgun methods, proteins were directly digested with proteases and the peptide mixtures were analyzed by one‐ or multi‐dimensional LC‐MS/MS . In 2DE‐based methods, proteins were separated on a two‐dimensional gel by the difference of apparent p I and mass, and the protein spots of interest were excised and analyzed as intact proteins or as peptides after in‐gel digestion, using MALDI‐MS or LC‐ESI‐MS . Reference searching revealed that though shotgun proteomics has gained increased popularity, so far 2DE‐MS has still been the main approach for rat plasma proteomic analysis, probably due to the powerful separation of 2DE for proteins in complex mixtures and the capacity to provide straightforward and visible information on the distributions of the intact proteins or polypeptides.…”

Section: Introductionmentioning

confidence: 99%

Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC‐MS/MS

Chen

Wen

et al. 2016

Electrophoresis

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Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a reference gel was selected, on which 136 CBB-stained spots were numbered and subjected to in-gel digestion and quantitative LC-MS/MS. The analysis provided the assignment of 1-25 (average eight) non-redundant proteins in each spot and totally 199 proteins were assigned in the 136 spots. About 40% of the proteins were detected in more than one spot and 15% in more than ten spots. We speculate this complexity arose from multiple causes, including protein heterogeneity, overlapping of protein locations and formation of protein complexes. Consequently, such results could not be appropriately presented as a conventional 2DE map, i.e. a list or a gel pattern with one or a few proteins annotated to each spot. Therefore, the LC-MS/MS quantity data was used to reconstruct the gel distribution of each protein and a library containing 199 native protein maps was established for rat plasma. Since proteins that formed a complex would migrate together during the nondenaturing 2DE and thus show similar gel distributions, correlation analysis was attempted for similarity comparison between the maps. The protein pairs showing high correlation coefficients included some well-known complexes, suggesting the promising application of native protein mapping for interaction analysis. With the importance of rat as the most commonly used laboratory animal in biomedical research, we expect this work would facilitate relevant studies by providing not only a reference library of rat plasma protein maps but a means for functional and interaction analysis.

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Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats

Cited by 11 publications

References 65 publications

Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex

Effect of duration of diabetes mellitus type 1 on properties of Na, K-ATPase in cerebral cortex

Sex Hormones Regulate Hepatic Fetuin Expression in Male and Female Rats

Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC‐MS/MS

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