Gender Dimorphism in High-Fat-Diet-Induced Insulin Resistance in Skeletal Muscle of Aged Rats

Gómez-Pérez, Yolanda; Amengual-Cladera, Emilia; Català-Niell, Antoni; Thomàs-Moyà, Elena; Gianotti, Magdalena; Proenza, Ana M.; Lladó, Isabel

doi:10.1159/000185538

Cited by 75 publications

(44 citation statements)

References 54 publications

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“…This difference was reflected in the greater divergence in body weight and liver adiposity between females fed the LF and HF diets. Similar gender dimorphic responses have been observed in rats fed HF diets (1,2) . These preliminary data provide evidence for sex-specific responses to high-fat intake but no apparent effect (up to 12 weeks of age) of the maternal low-folate insult.…”

supporting

confidence: 79%

Double insults: phenotypic consequences of folate depletion during development and high fat intake from weaning

et al. 2010

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The developmental origins of health and disease hypothesis propose that exposures during early life may contribute to increased risk of disease in adulthood. Growing evidence from animal models suggests that nutritional insults in utero result in altered programming of offspring, causing increased disease risk in later life. In a previous study, we observed that maternal folate depletion during pregnancy resulted in adult offspring which were heavier (P = 0.016) and had heavier organs (liver, P = 0.024; small intestine, P = 0.036) (unpublished data). The present study aimed to test the hypothesis that offspring born to folate-depleted mothers are more susceptible to increased adiposity and associated health consequences, particularly when fed a high-fat diet from weaning.Pairs of female C57BL/J6 mice were assigned randomly to folate-adequate (2 mg folic acid/kg) or folate-deplete (0.4 mg folic acid/kg) diets 4 weeks prior to mating. Dams remained on the allocated diets during pregnancy and lactation. At weaning (mean 23 d), offspring were allocated at random to a low (LF; 5 %) or high fat (HF; 20 %) diet, resulting in four treatment groups. Offspring were weighed weekly from weaning and food intake was assessed at 9 and 18 weeks of age. Total adiposity and liver fat deposition were assessed in a subset of mice (n 9 per treatment group) by MRI scanning at 12 and 24 weeks of age. Data were analysed using univariate ANOVA.At 9 weeks, food intake was not statistically different between treatment groups for female mice, but males given the HF diet postweaning ate 21.1 % less per week (P < 0.001) than those on the LF diet. Folate depletion during pregnancy and lactation did not significantly affect body weight, total adiposity or liver fat deposition in offspring at 12 weeks. At 12 weeks, female offspring fed the HF diet were 13.2 % heavier (P < 0.001) than those on the LF diet. Although HF-fed male offspring were 6.9 % heavier than those fed the LF diet, this difference was not statistically significant (P > 0.05). Total adiposity was increased (P < 0.001) in both male (23.0%) and female (30.2%) mice fed the HF diet from weaning. Liver fat tended to be higher in HF-fed female (23.2%, P = 0.059) and male (17 %, P = 0.284) mice.Folate depletion during development did not appear to influence appetite, body weight or adiposity at 12 weeks of age. However, HF feeding from weaning resulted in sex-specific phenotypes with the male mice appearing to have greater ability than the females to regulate food intake when exposed to the HF diet. This difference was reflected in the greater divergence in body weight and liver adiposity between females fed the LF and HF diets. Similar gender dimorphic responses have been observed in rats fed HF diets (1,2) . These preliminary data provide evidence for sex-specific responses to high-fat intake but no apparent effect (up to 12 weeks of age) of the maternal low-folate insult.We thank NuGO and the BBSRC (BB/G007993/1) for funding.

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supporting

confidence: 79%

Double insults: phenotypic consequences of folate depletion during development and high fat intake from weaning

et al. 2010

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“…Previous studies in our laboratory performed in several tissues point to rat gender differences in morphology and mitochondrial function [7,[14][15][16][17][18][19]. Taking this background into account, the aim of the present study was to investigate the gender-related differences in rat liver mitochondrial morphology, function and biogenesis in response to the overweight induced by chronic feeding with a HFD, imitating the dietary habit increasingly found in developing countries.…”

Section: Introductionmentioning

confidence: 95%

“…HFD feeding increases the amount of substrate available for oxidation and the electron flux through the mitochondrial respiratory chain by enhancing free radical production. In addition, an impairment of antioxidant defenses has also been reported in diet-induced obesity [7]. An excess of ROS is harmful to cells but, within a certain level, ROS may be involved in the communication between mitochondria and the nucleus controlling the mitochondrial biogenesis process [8].…”

Section: Introductionmentioning

confidence: 99%

Long-term High-fat-diet Feeding Impairs Mitochondrial Biogenesis in Liver of Male and Female Rats

Nadal-Casellas

Amengual-Cladera

Proenza

et al. 2010

Cell Physiol Biochem

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Background/Aims: Mitochondrial biogenesis includes both mitochondrial proliferation and differentiation and its regulation under different physiological conditions is not clear. Given the sexual dimorphism previously found in mitochondrial function, the aim of this study was to investigate the gender-dependent effect of chronic high-fat-diet (HFD) feeding on rat liver mitochondrial function and biogenesis. Methods: Ten-week old male and female rats were fed a HFD (26% fat) or a control diet (2.9% fat) for 26 weeks. Mitochondrial morphology was studied. Mitochondrial DNA and protein content, hydrogen peroxide production, oxidative capacity, antioxidant defenses, as well as markers of oxidative damage and mitochondrial biogenesis were analyzed. Results: Female rats showed higher levels of mitochondrial protein and an enhanced oxidative capacity per mitochondrion than males. In both genders, HFD feeding increased mtDNA content and decreased mitochondrial differentiation markers. Conclusion: In comparison to male rats, females show higher oxidative capacity as a consequence of their greater mitochondrial differentiation under both control and obese status. In response to HFD feeding, the oxidative capacity of the whole mitochondrial population is maintained in both genders. This is obtained by means of an enhancement of mitochondrial proliferation, which counteracts the diet-induced impairment of the function of each mitochondrion.

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“…Earlier studies demonstrated that sex dimorphism pointed towards a higher tendency of male rats to undergo metabolic syndrome manifestations when exposed to HFD feeding [19]. HFD feeding causes more detrimental effect in males due to higher insulin resistance, lower mitochondrial capacity, and lower facility to adapt to altered metabolic energy conditions [20].…”

Section: Introductionmentioning

confidence: 99%

Gender Dimorphism in Skeletal Muscle Proteome Between Lean and Diet-induced Obese Rats

Choi

et al. 2011

Cell Physiol Biochem

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Background: Although it has been believed for a long time that gender differences in physiology and metabolism were not relevant beyond the reproductive system, new research has indicated that sexual dimorphism may be more prevalent than previously believed. Therefore, the goal of this study was to develop a global view of the changes in gender-dependent protein abundance in two different types of skeletal muscles (soleus and gastrocnemius) of lean and high fat diet (HFD)-induced obese rats. Methods: To examine differential expression of proteins between gender and diet, we performed differential proteome analysis of skeletal muscle from lean and obese rats of both genders fed a HFD using 2-DE combined with MALDI-TOF-MS. Results: Our gender-specific proteome comparison showed that male and female rats displayed different patterns of proteome regulation including proteins involved in muscle contraction, carbohydrate and lipid metabolism, oxidative phosphorylation, as well as detoxification and antioxidant defenses. Conclusions: most of the candidate proteins identified herein by differential proteomics were previously unrecognized in gender dimorphism of skeletal muscle. Our data can serve as the basis for specific evidence-based interventions allowing for the prevention and treatment of obesity by matching the different needs of women and men such as the development of gender-based medicine.

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Gender Dimorphism in High-Fat-Diet-Induced Insulin Resistance in Skeletal Muscle of Aged Rats

Cited by 75 publications

References 54 publications

Double insults: phenotypic consequences of folate depletion during development and high fat intake from weaning

Double insults: phenotypic consequences of folate depletion during development and high fat intake from weaning

Long-term High-fat-diet Feeding Impairs Mitochondrial Biogenesis in Liver of Male and Female Rats

Gender Dimorphism in Skeletal Muscle Proteome Between Lean and Diet-induced Obese Rats

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