Gender differences in food allergy depend on the PPAR γ/NF-κB in the intestines of mice

Wang, Junjuan; Guo, Xin; Chen, Cheng; Sun, Shanfeng; Liu, Guirong; Liu, Manman; Hao, Mengzhen; Che, Huilian

doi:10.1016/j.lfs.2021.119606

Cited by 15 publications

(10 citation statements)

References 43 publications

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“…Gender is considered to be one of the factors causing food allergy, and female mice are generally considered to be more prone to allergy. , Wang et al . have demonstrated that female BALB/c mice were more vulnerable to food allergy . They found that systemic anaphylaxis in the food allergic mice was gender-dependent, and female mice treated with OVA exhibited a robust allergic reaction.…”

Section: Discussionmentioning

confidence: 99%

Study on the Sensitization and Antigenic Epitopes of Tropomyosin from Antarctic Krill (Euphausia superba)

Lin

Chi

et al. 2023

J. Agric. Food Chem.

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Antarctic krill (Euphausia superba), a shrimp-like marine crustacean, has become a beneficial source of high-quality animal protein. Meanwhile, a special focus has been placed on its potential sensitization issue. In this study, a 35 kDa protein was purified and identified to be Antarctic krill tropomyosin (AkTM) by high-performance liquid chromatography−tandem mass spectrometry (HPLC−MS/MS). The purified TM showed a strong IgE-binding capacity to shrimp/crab-allergic patients' sera, indicating that TM is the primary allergen in Antarctic krill. Simulated gastrointestinal digestion revealed that the digestion stability of TM to pepsin was higher than that to trypsin. The strong degranulation triggered by TM in RBL-2H3 cells suggested that AkTM has a strong sensitization capacity. The TM-sensitized BALB/c mice displayed severe anaphylactic symptoms; high levels of TMspecific IgE, sIgG1, and histamine; and increased IL-4, indicating that AkTM could provoke IgE-mediated allergic reactions. Bioinformatics prediction, indirect competition ELISA, and mast cell degranulation assay were used to map the antigenic epitopes of

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Section: Discussionmentioning

confidence: 99%

Study on the Sensitization and Antigenic Epitopes of Tropomyosin from Antarctic Krill (Euphausia superba)

Lin

Chi

et al. 2023

J. Agric. Food Chem.

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“…This is consistent with a previous study that showed that the female mice treated with OVA exhibited more serious systemic anaphylaxis than male mice. 26 Available clinical evidence suggests that sex differences likely play a role in food allergy development, for instance, by influencing the immune cells and mediators or the composition of the microbiome. 27 Here, more than four OVA-A-induced allergic reactions were successful (as evaluated by the diarrhea and allergy scores and the serum OVA-IgE levels).…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of l-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation

Gong

et al. 2023

Food Funct.

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“…In the study of OVA-A mechanisms, BALB/c mice are often used as model animals to study human-related diseases that contribute to the strong Th2 cytokine immune response. 30 Meanwhile, the OVA dose, the number of times OVA-A was induced, and the sex of the mice are crucial for successfully establishing an OVA-sensitized mouse model. Allergy-related clinical symptoms (diarrhea and allergy scores) and serum OVA-IgE levels were used to judge the effectiveness of the model.…”

Section: ■ Discussionmentioning

confidence: 99%

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Gong

et al. 2022

J. Agric. Food Chem.

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Ovalbumin (OVA), a common food protein, can cause deadly allergies with intestine-specific immune reactions. L-Theanine (LTA) shows great potential for regulating intestinal immunity. To investigate the regulatory effect of LTA intervention on intestine-specific immunity, a 41 day experiment was performed on BALB/c OVA-sensitized mice. The results show that injecting female mice intraperitoneally with 50 μg of OVA and administering 30 mg of OVA 4 times can successfully establish an OVAsensitized mouse model. LTA intervention significantly increased weight gain and thymus index (p < 0.05), decreased allergy and diarrhea scores (p < 0.05), and improved jejunum structure. Meanwhile, the histological score and degranulation of mast cells decreased. LTA intervention increased Clostridiales, Lachnospiraceae, Lactobacillus, Prevotella, and Ruminococcus abundance while decreasing Helicobacter abundance. Flow cytometry and Western blotting results indicated that 200 and 400 mg/kg of LTA upregulated the expression of T-bet and Foxp3 proteins (p < 0.05), thus promoting the differentiation of jejunum CD4+ T cells to Th1 and Tregs and increasing the cytokines IFN-γ, IL-10, and TGF-β (p < 0.05). We found that 200 and 400 mg/kg of LTA downregulated the expression of RORγt and GATA3, thus inhibiting the differentiation of Th2 and Th17 cells and decreasing cytokines IL-4, IL-5, IL-13 TNF-α, IL-6, and IL-17A (p < 0.05). LTA inhibited the degranulation of mast cells and significantly decreased the serum levels of OVA-IgE, HIS, and mouse MCPT-1 (p < 0.05). Therefore, LTA intervention alleviated OVA allergy by improving intestine-specific immunity.

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Gender differences in food allergy depend on the PPAR γ/NF-κB in the intestines of mice

Cited by 15 publications

References 43 publications

Study on the Sensitization and Antigenic Epitopes of Tropomyosin from Antarctic Krill (Euphausia superba)

Study on the Sensitization and Antigenic Epitopes of Tropomyosin from Antarctic Krill (Euphausia superba)

Synergistic effects of l-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

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