Gender determination from pulpal tissue

Abstract

Objective:To evaluate the diagnostic performance of X (Barr body [BB]) and Y (F body [FB]) chromosomes observed in dental pulp tissue for gender determination of an individual.Materials and Methods:The study was carried out on 100 teeth (50 male and 50 female), which were indicated for extraction. The teeth were sectioned at various intervals (within 12 h to 49 days post-extraction), and the pulpal tissue was obtained. Two slides for each pulp tissue were prepared, one for 5% Quinacrine dihydrochloride stain (FB) and the other for Hemotoxylin and Eosin stain (BB). The slides were then observed under the fluorescent microscope for FB and under the light microscope for the BB respectively.Results:Gender determination from human pulp is possible up to 7 weeks. The percentage of FB and BB decrease gradually as the time interval increases. Further, an equation was derived from the data based on the canonical discriminant function coefficients.Conclusion:The determination of gender based on a joint search for the presence or absence of X (BB) and Y (FB) Chromosome is a reliable and cost-effective technique.

Forensic Dentistry as an Analysis Tool for Sex Estimation: A Review of Current Techniques

“…Its usefulness in sex determination depends on the viability of the sample -Das et al found that sex can still be determined accurately from the study of XY chromosomes from mucosal epithelium up to a period of 4 weeks after death, 86 while Khorate et al found that sex determination from the human pulp was possible up to 7 weeks. 87 Reddy et al found that the dental pulp from female subjects showed the presence of Barr bodies up to a maximum of 400 degrees Celsius. 88 Galdames et al found that teeth subjected beyond 400 degrees Celsius did not provide any viable tissues for analysis 86 -demonstrating that even though the dental pulp is well-protected by the resilient dental hard tissues, it would not be able to survive all destructive conditions.…”

SRY gene isolation from teeth for forensic gender identification—An observational study

“…Though various features like crown size, and root lengths can differentiate male and female genders, instances with an absence of dental records and mutilated teeth samples make it impossible to solely rely on the morphological traits [ 2 ]. Therefore, in such instances, molecular analysis like DNA fingerprinting or DNA profiling is advantageous [ 3 ]. Notably, the extraction of DNA samples for gene expression analysis in gender determination has been studied.…”

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

“…So, the present study is planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination. 3 …”

“…So, the present study is planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination. 3 The aim of the present study is to assess the viability of human dental pulp as a source for DNA and to establish a reproducible, simple, and standardized protocol for age determination by polymerase chain reaction (PCR) method. We adopted O'Callaghan et al's modified method of absolute quantification of telomere length by introducing oligomer standard to generate telomere values.…”