Objective: We reported a canaliculitis case due to Gemella haemolysans and Porphyromonas asaccarolytica. Methods: After the diagnosis of canaliculitis, the unusual finding of yellowishgreenish material supplied from canalicular curettage and canaliculotomy was sent to microbiology laboratory for the presence of aerobic, anaerobic bacteria and fungi. Systemic and topical antimicrobial treatment was administered. Results: Bacteriological stains revealed polymorphonuclear leukocytes and gram-positive cocci. Facultative anaerobe Gemella haemolysans and anaerobe Porphyromonas asaccarolytica were identified according to bacteriological cultures. Routine cultures were negative for fungi. Recurrence was not observed at two years follow-up. Conclusion: To our knowledge, this is the first reported case of canaliculitis due to both Gemella haemolysans and Porphyromonas asaccarolytica and is also remarkable ocular infection case occurred after any surgery and/or trauma.