Gelation of gellan gum

Grasdalen, Hans; Smidsrød, Olav

doi:10.1016/0144-8617(87)90004-x

Cited by 351 publications

(172 citation statements)

References 21 publications

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…Similarly the secant modulus increased significantly when cation concentration was increased. Similar results for pure gellan gum gels [22] and pure alginate gels [23] have been previously reported. Vivoflow bioink was designed for cartilage bioprinting [14] and primary bovine chondrocytes were mixed into the ink prior to printing.…”

Section: Resultssupporting

confidence: 76%

Guidelines for standardization of bioprinting: a systematic study of process parameters and their effect on bioprinted structures

et al. 2016

View full text Add to dashboard Cite

Biofabrication techniques including threedimensional bioprinting could be used one day to fabricate living, patient-specific tissues and organs for use in regenerative medicine. Compared to traditional casting and molding methods, bioprinted structures can be much more complex, containing for example multiple materials and cell types in controlled spatial arrangement, engineered porosity, reinforcement structures and gradients in mechanical properties. With this complexity and increased function, however, comes the necessity to develop guidelines to standardize the bioprinting process, so printed grafts can safely enter the clinics. The bioink material must firstly fulfil requirements for biocompatibility and flow. Secondly, it is important to understand how process parameters affect the final mechanical properties of the printed graft. Using a gellan-alginate physically crosslinked bioink as an example, we show shear thinning and shear recovery properties which allow good printing resolution. Printed tensile specimens were used to systematically assess effect of line spacing, printing direction and crosslinking conditions. This standardized testing allowed direct comparison between this bioink and three commercially-available products. Bioprinting is a promising, yet complex fabrication method whose outcome is sensitive to a range of process parameters. This study provides the foundation for highly needed best practice guidelines for reproducible and safe bioprinted grafts.

show abstract

Section: Resultssupporting

confidence: 76%

Guidelines for standardization of bioprinting: a systematic study of process parameters and their effect on bioprinted structures

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The polymer backbone consists of glucose, glucuronic acid, and methyl pentose in the molar ratio 2:1:1 (39). Gelrite gels in the presence of monovalent or divalent cations (40,41). The mechanism of gelation involves the formation of doublehelical junction zones followed by aggregation of double-helical segments to form a 3D network by complexation with cations and hydrogen bonding with water (42).…”

Section: Rheologymentioning

confidence: 99%

In Situ Gelling Gelrite/Alginate Formulations as Vehicles for Ophthalmic Drug Delivery

Liu

Zhang

et al. 2010

AAPS PharmSciTech

View full text Add to dashboard Cite

Abstract. The objective of this study was to develop an ion-activated in situ gelling vehicle for ophthalmic delivery of matrine. The rheological properties of polymer solutions, including Gelrite, alginate, and Gelrite/alginate solution, were evaluated. In addition, the effect of formulation characteristics on in vitro release and in vivo precorneal drug kinetic of matrine was investigated. It was found that the optimum concentration of Gelrite solution for the in situ gel-forming delivery systems was 0.3% (w/w) and that for alginate solution was 1.4% (w/w). The mixture of 0.2% Gelrite and 0.6% alginate solutions showed a significant enhancement in gel strength at physiological condition. On the basis of the in vitro results, the Gelrite formulations of matrine-containing alginate released the drug most slowly. For each tested polymer solution, the concentration of matrine in the precorneal area was higher than that of matrinecontaining simulated tear fluid (STF) almost at each time point (p<0.05). The area under the curve of formulation 16 (0.2%Gelrite/0.6%alginate) was 4.65 times greater than that of containing matrine STF. Both the in vitro release and in vivo pharmacological studies indicated that the Gelrite/alginate solution had the better ability to retain drug than the Gelrite or alginate solutions alone. The tested formulation was found to be almost non-irritant in the ocular irritancy test. The overall results of this study revealed that the Gelrite/alginate mixture can be used as an in situ gelling vehicle to enhance ocular retention.

show abstract

“…10,11 Gellan gum is a polysaccharide that forms thermoreversible gels, being noncytotoxic in different tested situations. [12][13][14] It is commonly applied in the food industry, 15 and has been previously used in the biomedical field for drug delivery. 16 Gellan gum has been originally proposed by our group 17 as a new biomaterial for cartilage tissue engineering applications, yet recent studies can be found on the same application 18 and on the general use of gellan gum for tissue engineering.…”

mentioning

confidence: 99%

Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects

Oliveira

Gardel

Rada

et al. 2010

Journal Orthopaedic Research

126

104

View full text Add to dashboard Cite

In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellan gum with encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASC þ GF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellan gum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASC þ GF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASC þ GF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cells may constitute a valid alternative to currently used articular chondrocytes. ß

show abstract

Gelation of gellan gum

Cited by 351 publications

References 21 publications

Guidelines for standardization of bioprinting: a systematic study of process parameters and their effect on bioprinted structures

Guidelines for standardization of bioprinting: a systematic study of process parameters and their effect on bioprinted structures

In Situ Gelling Gelrite/Alginate Formulations as Vehicles for Ophthalmic Drug Delivery

Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects

Contact Info

Product

Resources

About