Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas

Forsyth, Peter; Wong, H; Laing, T D; Rewcastle, N. B.; Morris, D G; Muzik, Huong; Leco, K J; Johnston, Robert Mackenzie; Brasher, P M A; Sutherland, G; Edwards, Dylan R.

doi:10.1038/sj.bjc.6690291

Cited by 278 publications

(186 citation statements)

References 44 publications

(50 reference statements)

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“…MMP-9 was expressed in tumor cells and blood vessels and more strongly correlated with tumor grade [22]. We demonstrated on our astrocytoma collection significant positive correlation of MMP-9 expression and tumor grade which is in agreement with Pagestencher and Forsyth et al [21,22]. These data suggest that MMP-9 playes an important role in the pathophysiology of human gliomas and could be involved in both tumor invasion and angiogenesis.…”

Section: Discussionsupporting

confidence: 80%

“…MMP-9 was also observed higher in gliomas than in normal brain tissue. MMP-9 was expressed in tumor cells and blood vessels and more strongly correlated with tumor grade [22]. We demonstrated on our astrocytoma collection significant positive correlation of MMP-9 expression and tumor grade which is in agreement with Pagestencher and Forsyth et al [21,22].…”

Section: Discussionsupporting

confidence: 80%

“…Immunohistochemical detection showed that MMP-9 was localised to vessel walls, to neutrophils in areas of haemorrhage, and in glioblastomas [21]. Forsyth et al [22] analysed cDNA from 46 brain gliomas and showed significantly elevated levels of MMP-2 in low and high grade gliomas, in comparison with normal tissue. MMP-9 was also observed higher in gliomas than in normal brain tissue.…”

Section: Discussionmentioning

confidence: 99%

“…MMP-2 and MMP-9 members have been observed in gliomaderived cell lines [21]. Among MMPs, MMP-9 is particularly interesting because of a predominant localization in the region of angiogenesis [22]. There is some evidence that MMP-9 could be a potential angiogenic factor that signals through VEGF-VEGFR system [23].…”

mentioning

confidence: 99%

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Analysis of VEGF, Flt-1, Flk-1, nestin and MMP-9 in relation to astrocytoma pathogenesis and progression

Hlobílková¹,

Ehrmann²,

Knizetova³

et al. 2009

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Astrocytomas, particularly high grade astrocytoma, are brain tumors with potent angiogenic activity. Our immnunohistochemical study assessed vascular endothelial growth factor (VEGF), VEGF receptors (Flk-1, and Flt-1), the intermediate filamental protein nestin which plays a role in central nervous system development, and MMP-9, which belongs the family of matrix metalloproteinases implicated in tumor invasion and angiogenesis regulation. We investigated the expression of VEGF, its receptors, nestin and MMP-9 in astrocytomas and their correlation with tumor grade. We used paraffin-embedded samples from 66 patients, 29 with low grade (WHO-grade II) and 37 with high grade (WHO-grade III and IV) astrocytomas. Antibodies against VEGF, Flk-1, Flt-1, nestin, CD34 and MMP-9 were used, followed by standard indirect immunohistochemical methods. Expression of Flt-1 and Flk-1 showed no significant differences between low and high grade tumor groups. Expression of VEGF and MMP-9 was increased in the high grade group (p equal to or less than 0.026 and 0.024). Nestin expression in tumor astrocytes and endothelial cells increased in high grade group (p same 0.007 and 0.003). Higher expression of VEGF in high grade astrocytomas may subsequently lead to activation of survival, angiogenesis and migration. Expression of nestin and MMP-9 also suggest their likely role in astrocytoma vascular development and proliferation.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of VEGF, Flt-1, Flk-1, nestin and MMP-9 in relation to astrocytoma pathogenesis and progression

Hlobílková¹,

Ehrmann²,

Knizetova³

et al. 2009

neo

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“…We used gelatin zymography to measure the enzymatic activity of matrix metalloproteinase-2 (MMP-2), a recognized endoprotease in glioma invasion [37], in media harvested after CQ treatments of GBM cells. The experiments revealed concentration-dependent reductions of MMP-2 activity in supernatants of CQ-treated primary cultures and cell lines; since we hypothesize that CQ's effect are partly owed to reduce TGF-β secretion, we added a rescue condition by supplementing 25 μM CQ with 1 ng/ml rhTGF-β 2 ( Fig.…”

Section: Chloroquine Inhibits Matrix Metalloproteinase-2 Activity Andmentioning

confidence: 99%

Chloroquine inhibits the malignant phenotype of glioblastoma partially by suppressing TGF-beta

Roy¹,

Poirier²,

Fortin³

2015

Invest New Drugs

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Association of Rosacea With Risk for Glioma in a Danish Nationwide Cohort Study

et al. 2016

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Gelatinase-A (MMP-2), gelatinase-B (MMP-9) and membrane type matrix metalloproteinase-1 (MT1-MMP) are involved in different aspects of the pathophysiology of malignant gliomas

Cited by 278 publications

References 44 publications

Analysis of VEGF, Flt-1, Flk-1, nestin and MMP-9 in relation to astrocytoma pathogenesis and progression

Analysis of VEGF, Flt-1, Flk-1, nestin and MMP-9 in relation to astrocytoma pathogenesis and progression

Chloroquine inhibits the malignant phenotype of glioblastoma partially by suppressing TGF-beta

Association of Rosacea With Risk for Glioma in a Danish Nationwide Cohort Study

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