Gelatin-templated biomimetic calcification for β-galactosidase immobilization

Kluyveromyces lactis β-galactosidase immobilization in calcium alginate spheres and gelatin for hydrolysis of cheese whey lactose

“…This result was higher than the one obtained by ESCOBAR et al (2014), who immobilized K. lactis β-galactosidase in calcium alginate spheres and achieved a maximum lactose conversion of 40% during the same reaction time. The highest conversion obtained in this research may be attributed to the use of gelatin, which decreased swelling of the alginate spheres, minimizing the leaching of the enzyme from the support to the external medium (SHEN et al, 2011).…”

Synthesis of a hybrid polymer-inorganic biomimetic support incorporating in situ pectinase from Aspergillus niger ATCC 9642

“…The immobilization methodology used in this work was adapted from Shen et al [16]. Initially, a 2 % (p/v) sodium alginate solution dissolved in sodium oxalate buffer (100 mM, pH 5.5) was prepared.…”

“…Shen et al [16] used potassium phosphate buffer (100 mM, pH 7.5) to obtain a polymer-inorganic support for encapsulation of b-galactosidase. Preliminary tests performed here (data not shown) demonstrated that potassium phosphate buffer led to the total loss of activity for the pectinolytic extract.…”

Synergistic effects of amine and protein modified epoxy-support on immobilized lipase activity

“…To overcome this limitation, researchers have adopted many methods, one of which is to modify the support surface with biofriendly molecules to obtain the biocompatible surface to reduce the enzyme denaturation during the immobilization [32][33][34]. There are many methods to form such biocompatible ("bio-friendly") support surfaces, including coating, adsorption, and self-assembly [35,36].…”