Gelatin/monetite electrospun scaffolds to regenerate bone tissue: Fabrication, characterization, and in-vitro evaluation

Singh, Yogendra Pratap; Mishra, Balaram; Gupta, Mukesh Kumar; Bhaskar, Rakesh; Han, Sung Soon; Mishra, Narayan Chandra; Dasgupta, Sudip

doi:10.1016/j.jmbbm.2022.105524

Cited by 12 publications

(7 citation statements)

References 35 publications

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“…At specific time intervals (7, 14, and 21 days for degradation in PBS solution and 1, 2, 4, 6, and 8 h for degradation in PBS solution containing lysozyme), the samples were removed from the degradation environment and weighed after 24 h of exposure to air (w 2 ). The percentage of degradation of scaffolds at any specified time was calculated using eq degradation % = W 1 − W 2 W 1 × 100 …”

Section: Methodsmentioning

confidence: 99%

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Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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Platelet-derived growth factor-BB (PDGF-BB) is a polypeptide growth factor generated by platelet granules faced to cytokines. It plays a role in forming and remodeling various tissue types, including epithelial tissue, through interaction with cell-surface receptors on most mesenchymal origin cells. However, it breaks down quickly in biological fluids, emphasizing the importance of preserving them from biodegradation. To address this challenge, we formulated and evaluated PDGF-encapsulated nanospheres (PD@PCEC) using polycaprolactone-polyethylene glycol-polycaprolactone. PD@PCECs were fabricated through the triple emulsion methodology and optimized by using the Box− Behnken design. The encapsulation efficiency (EE) of nanoencapsulated PDGF-BB was investigated concerning four variables: stirring rate (X1), stirring duration (X2), poly(vinyl alcohol) concentration (X3), and PDGF-BB concentration (X4). The selected optimized nanospheres were integrated into a gelatin-collagen scaffold (PD@PCEC@GC) and assessed for morphology, biocompatibility, in vitro release, and differentiation-inducing activity in human adipose-derived stem cells (hADSCs). The optimized PD@PCEC nanospheres exhibited a particle size of 177.9 ± 91 nm, a zeta potential of 5.2 mV, and an EE of 87.7 ± 0.44%. The release profile demonstrated approximately 85% of loaded PDGF-BB released during the first 360 h, with a sustained release over the entire 504 h period, maintaining bioactivity of 87.3%. The study also included an evaluation of the physicochemical properties of the scaffolds and an assessment of hADSC adhesion to the scaffold's surface. Additionally, hADSCs cultivated within the scaffold effectively differentiated into keratinocyte-like cells (KLCs) over 21 days, evidenced by morphological changes and upregulation of keratinocyte-specific genes, including cytokeratin 18, cytokeratin 19, and involucrin, at both transcriptional and protein levels.

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Section: Methodsmentioning

confidence: 99%

“…The percentage of degradation of scaffolds at any specified time was calculated using eq 4. 48 = × W W W degradation% 100…”

mentioning

confidence: 99%

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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“…Many researchers reported, besides biochemical composition and biocompatibility, other properties must be taken into account in choosing inorganic phase of artificial ECM-like structure [23]. Our Previous research work [13] gelatin/monetite electrospun scaffolds using electrospinning technique reported higher cell viability, adhesion, and spreading tendency in scaffolds with 5-7 wt% monetite content into the gelatin matrix. The alkaline phosphatase (ALP) activity and the elongation of MG-63 cells were found to be positively correlated with the monetite concentration, resulting in a spatial gradient of cell phenotypes.…”

Section: Introductionmentioning

confidence: 95%

“…Further, gelatin is also a natural polymer, derived from the thermal denaturation of collagen. Gelatin is biodegradable, biocompatible, does not stimulate immune-rejection reactions, and maintains molecular cues that control cellular behavior [12,13]. It possess extensive applications in the field of biomedical engineering and highly elctrospinnable which as a core, can increase the spin-ability of chitosan [14].…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and bioactivity of reinforced monetite with chitosan-gelatin electrospun composite scaffold for bone tissue engineering

Singh

Purohit²,

Gupta

et al. 2023

Biomed. Mater.

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In this study, chitosan-gelatin-monetite (CGM)-based electrospun scaffolds have been developed that closely mimicked the microstructure and chemical composition of the extracellular matrix of natural bone. CGM-based nanofibrous composite scaffolds were prepared with the help of the electrospinning technique, post-cross-linked using EDC and NHS solution to improve their stability in an aqueous environment. The prepared CG scaffold showed an average fiber diameter of 284 ± 17 nm, whereas 5 and 7 wt% monetite containing CGM5 and CGM7 scaffolds, exhibited an average fiber diameter of 390 ± 11 and 435 ± 15 nm, respectively, revealing the fine distribution of monetite particles on the fibrous surface. The distribution of monetite nanoparticles onto the CG nanofibrous surface was confirmed using XRD, FTIR, and EDAX. Moreover, the addition of 7 wt% monetite into the CG electrospun matrix increased their ultimate tensile strength from 2.01 ± 0.05 MPa in the CG scaffold to 11.68 ± 0.09 MPa in the CGM7 scaffold. SBF study and staining with ARS confirmed the higher mineralization ability of monetite-containing scaffolds compared to that revealed by the CG scaffold. The monetite incorporation into the CG matrix improved its osteogenic properties, including pre-osteoblast MG-63 cell adhesion, proliferation, and differentiation, when seeded with the cells. A higher degree of cellular adhesion, spreading, and migration was observed on the monetite-incorporated CG scaffold than that on the CG scaffold. From MTT assay, ALP activity, ARS staining, and immunocytochemistry study, the cultured cells discovered a more conducive microenvironment to proliferate and subsequently differentiate into osteoblast lineage in contact with CGM7 nanofibers rather than that in CGM0 and CGM5. In-vitro results indicated that electrospun CGM-based composite scaffolds could be used as a potential candidate to repair and regenerate new bone tissues.

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“…Currently, electrospun gelatin scaffolds in combination with other polymers have been developed for wound healing applications ( Ajmal et al, 2023 ; Lashkari et al, 2023 ; Zhang et al, 2023 ), nervous system tissue ( Liu et al, 2021 ; Talebi et al, 2021 ; Zamanifard et al, 2023 ), dental applications ( Sharifi et al, 2022 ; Acuña et al, 2023 ), bone tissue ( Sun et al, 2022 ; Singh et al, 2023 ) and skin tissue ( Baghersad et al, 2022 ; Farshi et al, 2022 ; Ghomi et al, 2023 ; Khalilimofrad et al, 2023 ), tendon implants ( Wang et al, 2022 ; Xue et al, 2022 ) and vascular grafts ( Zhu et al, 2020 ; Jiang et al, 2021 ; Fahad et al, 2023 ). All these studies have the characteristic of blending different synthetic polymers with gelatin to overcome shortcomings of both materials ( Chong et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Can we achieve biomimetic electrospun scaffolds with gelatin alone?

Roldán

Reeves

Cooper

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Gelatin is a natural polymer commonly used in biomedical applications in combination with other materials due to its high biocompatibility, biodegradability, and similarity to collagen, principal protein of the extracellular matrix (ECM). The aim of this study was to evaluate the suitability of gelatin as the sole material to manufacture tissue engineering scaffolds by electrospinning.Methods: Gelatin was electrospun in nine different concentrations onto a rotating collector and the resulting scaffold’s mechanical properties, morphology and topography were assessed using mechanical testing, scanning electron microscopy and white light interferometry, respectively. After characterizing the scaffolds, the effects of the concentration of the solvents and crosslinking agent were statistically evaluated with multivariate analysis of variance and linear regressions.Results: Fiber diameter and inter-fiber separation increased significantly when the concentration of the solvents, acetic acid (HAc) and dimethyl sulfoxide (DMSO), increased. The roughness of the scaffolds decreased as the concentration of dimethyl sulfoxide increased. The mechanical properties were significantly affected by the DMSO concentration. Immersed crosslinked scaffolds did not degrade until day 28. The manufactured gelatin-based electrospun scaffolds presented comparable mechanical properties to many human tissues such as trabecular bone, gingiva, nasal periosteum, oesophagus and liver tissue.Discussion: This study revealed for the first time that biomimetic electrospun scaffolds with gelatin alone can be produced for a significant number of human tissues by appropriately setting up the levels of factors and their interactions. These findings also extend statistical relationships to a form that would be an excellent starting point for future research that could optimize factors and interactions using both traditional statistics and machine learning techniques to further develop specific human tissue.

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Gelatin/monetite electrospun scaffolds to regenerate bone tissue: Fabrication, characterization, and in-vitro evaluation

Cited by 12 publications

References 35 publications

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Preparation, characterization, and bioactivity of reinforced monetite with chitosan-gelatin electrospun composite scaffold for bone tissue engineering

Can we achieve biomimetic electrospun scaffolds with gelatin alone?

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