Gelatin Hydrogel With Basic Fibroblast Growth Factor for Tympanic Membrane Regeneration

Abstract

These data suggest that hydrogel impregnated with bFGF induces regeneration of the tympanic membrane and can conservatively treat tympanic membrane perforation.

Regeneration of the tympanic membrane using fibroblast growth factor-2

“…The best dosage and application time for fibroblast growth factor-2 for the regeneration of tympanic membrane perforations are unclear. The application dosage and time given in some previous studies are shown in Table IV 4 – 9 , 11 – 32 . The dosage in these studies was not uniform.…”

“…Some scholars reported that the fibroblast growth factor-2 group showed a significantly improved closure rate and shorted the closure time in subjects with acute tympanic membrane perforations compared with the phosphate-buffered saline treated group or glycerol group 4 , 5 , 7 – 9 . In addition, fibroblast growth factor-2 treatment also promotes the healing of glucocorticoid-induced tympanic membrane perforations 39 , 40 .…”

No systemic exposure of transtympanic heparin-binding epidermal growth factor like growth factor

“…(Santa Maria et al, Santa Maria et al, 2015) The other concern for growth factor treatments for the tympanic membrane is the potential to induce cholesteatoma or malignancy formation, but this is yet to be observed in any animal or human studies of tympanic membrane growth factor treatment. (Acharya et al, 2015, Hakuba et al, 2010, Hakuba et al, 2014, Hakuba et al, 2003, Kanemaru et al, 2011, Ramalho and Bento, 2006, Ma et al, 2002, Dvorak et al, 1995, Santa Maria et al, 2015) Based on all available research to date, the application of trans tympanic HB-EGF is safe to the local tissues. A phase one clinical trial will be needed to definitively answer this question.…”

Tissue Engineering for Otorhinolaryngology–Head and Neck Surgery

“…All the animals in the lattermost group developed a fibrous layer in addition to an epithelial and mucosal layer, with complete closure of the perforation. 70 This study is one of many to use bFGF as a bioactive molecule to aid in the closure of tympanic membrane perforations. [71][72][73] In addition to gelatin hydrogels, absorbable gelatin compressed sponge (Gelfoam, Pfizer), HA, decellularized dermis, chitosan, alginate, and even rice paper have been explored as potential scaffolds, with the bulk of the literature exploring epidermal growth factor and FGF families as bioactive molecules.…”