Gelatin–genipin‐based biomaterials for skeletal muscle tissue engineering

Gattazzo, Francesca; Maria, Carmelo De; Rimessi, Alessandro; Donà, Silvio; Braghetta, Paola; Pinton, Paolo; Vozzi, Giovanni; Bonaldo, Paolo

doi:10.1002/jbm.b.34057

Cited by 48 publications

(37 citation statements)

References 73 publications

(125 reference statements)

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“…Genipin was first utilized as a crosslinker in 1988 and since then its use has been studied for a wide array of applications [29]. In materials as diverse as the trachea, pericardium, cartilage, chitosan and gelatin gels, genipin has been successfully utilized to increase the mechanical strength and reduce antigenicity [8,[30][31][32][33]. Our study correlates with others on the ability of genipin to attach functionalized AuNPs to the tissue.…”

Section: Discussionsupporting

confidence: 72%

Genipin Attachment of Conjugated Gold Nanoparticles to a Decellularized Tissue Scaffold

et al. 2019

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Decellularized allograft tissue is used for a wide array of tissue injuries and repair with tenons and ligament repair being among the most common. However, despite their frequent use there is concern over the lengthy inflammatory period and slow healing associated with allografts. One promising solution has been the use of nanoparticles. There is currently no easy, fast method to achieve consistent conjugation of nanoparticles to tissue. The available conjugation methods can be time-consuming and/or may create numerous cytotoxic byproducts. Genipin, a naturally derived crosslinking agent isolated from the fruits of Gardenia jasminoides was investigated as a conjugation agent to achieve fast, consistent crosslinking without cytotoxic byproducts. The rational of utilizing genipin is that is reacts spontaneously with amino-group-containing compounds such as proteins, collagens, and gelatins, and does not require extensive washing after conjugation. Porcine diaphragm tendons were decellularized and then immersed in cysteamine functionalized gold nanoparticles and genipin for various time points. Tissue scaffolds were tested for the degree of crosslinking, gold nanoparticle concentrations, and fibroblast attachment and biocompatibility. Results demonstrated that genipin can successfully and reproducibly attach gold nanoparticles to tissue in as little as 15 min. The genipin had no cytotoxic effects and improved fibroblast attachment and proliferation. Genipin can be used to attach gold nanoparticles to tissue in a fast, cell safe manner.

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Section: Discussionsupporting

confidence: 72%

Genipin Attachment of Conjugated Gold Nanoparticles to a Decellularized Tissue Scaffold

et al. 2019

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“…Indeed, as biomaterials for tissue engineering, a wide diversity of gelatin-based structures have been described in literature 17 including nano-and microspheres 18,19 or particles, 3D scaffolds [20][21][22][23][24] , electrospun nanofibers [25][26][27] , cryogel scaffolds 28 , composite materials [29][30][31] and in situ gelling formulations. This reverts in a broad range of applications going from drug and growth factors delivery 18,19,30 to tissue repair and regeneration for ocular 23 , bone 30 , skeletal muscle 32 or soft tissue 24,31 engineering.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Generation of Reactive Species by Cold Plasma in Gelatin Solutions for Selective Cancer Cell Death

Labay

Roldán

Tampieri

et al. 2020

ACS Appl. Mater. Interfaces

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Atmospheric pressure plasma jets generate reactive oxygen and nitrogen species (RONS) in liquids and biological media, which find application in the new area of plasma medicine. These plasma-treated liquids demonstrated recently to possess selective properties on killing cancer cells and attract attention towards new plasma-based cancer therapies. These allow for local delivery by injection in the tumor but can be quickly washed away by body fluids. By confining these RONS in a suitable biocompatible delivery system, great perspectives can be opened in the design of novel biomaterials aimed for cancer therapies. Gelatin solutions are evaluated here to store RONS generated by atmospheric pressure plasma jets, and their release properties are evaluated. The concentration of RONS was studied in 2% gelatin as a function of different plasma parameters (treatment time, nozzle distance and gas flow) with two different plasma jets. Much higher production of reactive species (H 2 O 2 and NO 2-) was revealed in the polymer solution than in water after plasma treatment. The amount of RONS generated in gelatin is greatly improved with respect to water, with concentrations of H 2 O 2 and NO 2between 2 and 12 times higher for the longest plasma treatments. Plasma-treated gelatin exhibited the release of these RONS to a liquid media, which induced an effective killing of bone cancer cells. Indeed, in vitro studies on Sarcoma Osteogenic (SaOS-2) cell line exposed to plasma-treated gelatin lead to timedependent increasing cytotoxicity with the longer plasma treatment time of gelatin. While SaOS-2 cell viability decreased down to 12%-23% after 72 hours for cells exposed to 3-min treated gelatin, viability of healthy cells (hMSC) was preserved (around 90%), establishing the selectivity of the plasma-treated gelatin on cancer cells. This sets the basis for designing improved hydrogels with high capacity to deliver RONS locally to tumors.

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“…Gelatin is a mixture of peptide hydrolysed from collagen and has been widely used in food, pharmaceutical and cosmetic industries as a stabilizing ingredient since it is non-toxic, biocompatible, and biodegradable. In tissue engineering and biomedicine, gelatin-based hydrogels with porous network structures can provide an ideal environment for cells attachment and promote their colonization, migration and proliferation [6][7][8] . The gelation of mammalian and warm-water fish gelatin in aqueous solution occurs at temperature below 25 °C due to physical folding of the polypeptide backbone, i.e.…”

mentioning

confidence: 99%

High strength and low swelling composite hydrogels from gelatin and delignified wood

Wang

Zhou

2020

Sci Rep

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A delignified wood template with hydrophilic characteristics and high porosity was obtained by removal of lignin. Gelatin was infiltrated into the delignified wood and further crosslinked with a natural crosslinker genipin to form hydrogels. The composite hydrogels showed high mechanical strength under compression and low swelling in physiological condition. The effect of genipin concentrations (1, 50 and 100 mM) on structure and properties of the composite hydrogels were studied. A porous honeycomb structure with tunable pore size and porosity was observed in the freeze-dried composite hydrogels. High elastic modulus of 11.82 ± 1.51 MPa and high compressive yield stress of 689.3 ± 34.9 kPa were achieved for the composite hydrogel with a water content as high as 81%. The equilibrium water uptake of the freeze-dried hydrogel in phosphate buffered saline at 37 °C was as low as 407.5%. These enables the delignified wood structure an excellent template in composite hydrogel preparation by using infiltration and in-situ synthesis, particularly when high mechanical strength and stiffness are desired.

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Gelatin–genipin‐based biomaterials for skeletal muscle tissue engineering

Cited by 48 publications

References 73 publications

Genipin Attachment of Conjugated Gold Nanoparticles to a Decellularized Tissue Scaffold

Genipin Attachment of Conjugated Gold Nanoparticles to a Decellularized Tissue Scaffold

Enhanced Generation of Reactive Species by Cold Plasma in Gelatin Solutions for Selective Cancer Cell Death

High strength and low swelling composite hydrogels from gelatin and delignified wood

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