Gelatin Effects on the Physicochemical and Hemocompatible Properties of Gelatin/PAAm/Laponite Nanocomposite Hydrogels

Li, Changpeng; Mu, Changdao; Lin, Wei; Ngai, To

doi:10.1021/acsami.5b05287

Cited by 118 publications

(90 citation statements)

References 52 publications

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“…As we can see, 6NC50 and 6NC50-NIPAM show typical bands of the main component. The stretching bands observed at 2931 and 1663 cm −1 correspond to C-H and C=O of carboxamide [35]. The characteristic peak related to Si-O stretching of laponite component was detected at 1003 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

2018

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In this study, we fabricated and characterized a smart shear-thinning hydrogel composed of gelatin and laponite for localized drug delivery. We added chitosan (Chi) and poly N-isopropylacrylamide-co-Acrylic acid (PNIPAM) particles to the shear-thinning gel to render it pH-responsive. The effects of total solid weight and the percentage of laponite in a solid mass on the rheological behavior and mechanical properties were investigated to obtain the optimum formulation. The nanocomposite gel and particles were characterized using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), zeta potential, and dynamic light scattering techniques. Finally, release related experiment including degradability, swelling and Rhodamine B (Rd) release at various pH were performed. The results suggest that incorporation of silicate nanoplatelets in the gelatin led to the formation of the tunable porous composite, with a microstructure that was affected by introducing particles. Besides, the optimum formulation possessed shear-thinning properties with modified rheological and mechanical properties which preserved its mechanical properties while incubated in physiological conditions. The release related experiments showed that the shear-thinning materials offer pH-sensitive behavior so that the highest swelling ratio, degradation rate, and Rd release were obtained at pH 9.18. Therefore, this nanocomposite gel can be potentially used to develop pH-sensitive systems.

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Section: Resultsmentioning

confidence: 99%

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

2018

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“…8. 19 Aer the introduction of LAPONITE®, the compressive stress-strain properties for all the gels are improved, as shown in Fig. Hence, MA-gelatin can not only act as a macromolecular crosslinker for the hydrogel preparation, but can also contribute to the construction of the rigid structure.…”

Section: Swelling Behavioursmentioning

confidence: 91%

“…19 All the measurements were carried out on an MTS Universal Testing Machine (CMT6202, MTS systems Co., Ltd., China) at room temperature. 19 All the measurements were carried out on an MTS Universal Testing Machine (CMT6202, MTS systems Co., Ltd., China) at room temperature.…”

Section: Measurements Of Mechanical Propertiesmentioning

confidence: 99%

“…It has been recognized that gelatin is able to improve cell adhesion and proliferation, [14][15][16] and inhibit platelet adhesion and thrombosis generation. 19 However, the incorporated gelatin chains would be released from the gel network during sustained exposure to an aqueous environment, since they are immobilized in the hydrogel only by noncovalent interactions. 19 However, the incorporated gelatin chains would be released from the gel network during sustained exposure to an aqueous environment, since they are immobilized in the hydrogel only by noncovalent interactions.…”

Section: Introductionmentioning

confidence: 99%

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Novel hemocompatible nanocomposite hydrogels crosslinked with methacrylated gelatin

Lin

2016

RSC Adv.

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Methacrylated gelatin (MA-gelatin) is a gelatin derivative synthesized by the reaction with methacrylic anhydride; the degree of substitution (DS) of primary amines by methacrylamide groups in the gelatin is closely related to the dosage of methacrylic anhydride. In this work, MA-gelatin has been developed as a macromolecular crosslinker to prepare novel nanocomposite hydrogels (NC gels) based on polyacrylamide (PAAm) and LAPONITE® nanoclay. Compared with unmodified gelatin, MA-gelatin improves the stability of the LAPONITE® suspension in the pre-gel system. FTIR results confirm the success of gelatin modification, in agreement with the 1 H NMR result, and the copolymerization of MA-gelatin and acrylamide monomers in the NC gel network. The increasing DS of MA-gelatin thus reduces the equilibrium swelling ratio (ESR) and pore sizes, and enhances the mechanical properties of the NC gels, due to the macromolecular crosslinking effect. Unlike the small molecular crosslinker N,N 0 -methylenebisacrylamide (BIS), MA-gelatin shows a stronger influence on the swelling behaviours and mechanical properties of NC gels. Moreover, the MA-gelatin crosslinked NC gels exhibit decreased bovine serum albumin (BSA) adsorption, prolonged blood-clotting time and nonhemolytic nature, indicating the improved antithrombogenicity. The results show that MA-gelatin can be a hemocompatible macromolecular crosslinker for the fabrication of biomedical materials.

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“…26–30 PNIPAM can be used as a thermo-responsive matrix; however, due to biostability of PNIPAM, it is not readily cleared from the body under physiological conditions. 24 To overcome this issue, a range of hydrogels are synthesized by combining PNIPAM and degradable functional groups 31 or polymers 32–34 .…”

Section: Introductionmentioning

confidence: 99%

Injectable nanoengineered stimuli-responsive hydrogels for on-demand and localized therapeutic delivery

et al. 2017

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“Smart” hydrogels are an emerging class of biomaterials that respond to external stimuli and have been investigated for a range of biomedical applications, including therapeutic delivery and regenerative engineering. Stimuli-responsive nanogels constructed of thermoresponsive polymers such as poly(N-isopropylacrylamide-co-acrylamide) (poly(NIPAM-co-AM)) and magnetic nanoparticles (MNPs), have been developed as “smart carriers” for on-demand delivery of therapeutic biomolecules via magneto-thermal activation. However, due to their small size and systemic introduction, these poly(NIPAM-co-AM)/MNPs nanogels result in limited control over long-term, localized therapeutic delivery. Here, we developed an injectable nanoengineered hydrogel loaded with poly(NIPAM-co-AM)/MNPs for localized, on-demand delivery of therapeutics (doxorubicin (DOX)). We have engineered shear-thinning and self-recoverable hydrogels by modulating the crosslinking density of a gelatin methacrylate (GelMA) network. poly(NIPAM-co-AM)/MNP nanogels loaded with DOX were entrapped within a GelMA pre-polymer solution prior to crosslinking. The temperature and magnetic field dependent release of loaded DOX was observed from the nanoengineered hydrogels (GelMA/(poly(NIPAM-co-AM)/MNPs)). Finally, the in vitro efficacy of DOX released from injectable nanoengineered hydrogels was investigated using preosteoblast and osteosarcoma cells. Overall, these results demonstrated that the injectable nanoengineered hydrogels could be used for on-demand and localized therapeutic delivery for biomedical applications.

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Gelatin Effects on the Physicochemical and Hemocompatible Properties of Gelatin/PAAm/Laponite Nanocomposite Hydrogels

Cited by 118 publications

References 52 publications

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

Smart Shear-Thinning Hydrogels as Injectable Drug Delivery Systems

Novel hemocompatible nanocomposite hydrogels crosslinked with methacrylated gelatin

Injectable nanoengineered stimuli-responsive hydrogels for on-demand and localized therapeutic delivery

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