Gelatin coating promotes <i>in situ</i> endothelialization of electrospun polycaprolactone vascular grafts

Xing, Yuehao; Gu, Yongquan; Guo, Lu; Guo, Jianming; Xu, Zeqin; Xiao, Yonghao; Fang, Zhiping; Feng, Zeng‐guo; Wang, Zhonggao

doi:10.1080/09205063.2021.1909413

Cited by 23 publications

(19 citation statements)

References 57 publications

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“…As shown in Figure 2 B, the tensile strength of PET, PU@PET, Gel@PET, and PU-Gel@PET were 1.32 MPa, 1.45 MPa, 1.26 MPa, and 1.52 MPa, respectively. This parameter is about 9 times that of Xing’s work [ 34 ], whose burst tensile is approximately 1000 mmHg, while the burst tensile of swine blood vessels is between 2000~4000 mmHg [ 32 ]. Therefore, the sample we prepared met the requirement.…”

Section: Resultsmentioning

confidence: 99%

Improving Biocompatibility of Polyester Fabrics through Polyurethane/Gelatin Complex Coating for Potential Vascular Application

et al. 2022

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Medical apparatus and instruments, such as vascular grafts, are first exposed to blood when they are implanted. Therefore, blood compatibility is considered to be the critical issue when constructing a vascular graft. In this regard, the coating method is verified to be an effective and simple approach to improve the blood compatibility as well as prevent the grafts from blood leakage. In this study, polyester fabric is chosen as the substrate to provide excellent mechanical properties while a coating layer of polyurethane is introduced to prevent the blood leakage. Furthermore, gelatin is coated on the substrate to mimic the native extracellular matrix together with the improvement of biocompatibility. XPS and FTIR analysis are performed for elemental and group analysis to determine the successful coating of polyurethane and gelatin on the polyester fabrics. In terms of blood compatibility, hemolysis and platelet adhesion are measured to investigate the anticoagulation performance. In vitro cell experiments also indicate that endothelial cells show good proliferation and morphology on the polyester fabric modified with such coating layers. Taken together, such polyester fabric coated with polyurethane and gelatin layers would have a promising potential in constructing vascular grafts with expected blood compatibility and biocompatibility without destroying the basic mechanical requirements for vascular applications.

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Section: Resultsmentioning

confidence: 99%

Improving Biocompatibility of Polyester Fabrics through Polyurethane/Gelatin Complex Coating for Potential Vascular Application

et al. 2022

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“…[21,22] In addition to the above advantages, gelatin coating can also promote in situ endothelialization of electrospun vascular grafts. [23] To sum up, this study attempts to design and fabricate coaxially-structured fibers with upstanding mechanical and biological properties, which could combine the advantages of polyester materials and natural biological materials. PU was utilized as inner core to provide mechanical support, while biodegradable natural biomaterial gelatin acted as a shell to provide a surface for cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…[ 21 , 22 ] In addition to the above advantages, gelatin coating can also promote in situ endothelialization of electrospun vascular grafts. [ 23 ]…”

Section: Introductionmentioning

confidence: 99%

Design and characterization of small-diameter tissue-engineered blood vessels constructed by electrospun polyurethane-core and gelatin-shell coaxial fiber

et al. 2021

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Substitution or bypass is the most effective treatment for vascular occlusive diseases.The demand for artificial blood vessels has seen an unprecedented rise due to the limited supply of autologous blood vessels. Tissue engineering is the best approach to provide artificial blood vessels. In this study, a new type of small-diameter artificial blood vessel with good mechanical and biological properties was designed by using electrospinning coaxial fibers. Four groups of coaxial fibers vascular membranes having polyurethane/gelatin core-shell structure were cross-linked by the EDC-NHS system and characterized. The core-shell structure of the coaxial vascular fibers was observed by transmission electron microscope. After the crosslinking, the stress and elastic modulus increased and the elongation decreased, burst pressure of 0.11 group reached the maximum (2844.55 ± 272.65 mmHg) after cross-linking, which acted as the experimental group. Masson staining identified blue-stained ring or elliptical gelatin ingredients in the vascular wall. The cell number in the vascular wall of the coaxial group was found in muscle embedding experiment significantly higher than that of the non-coaxial group at all time points (p < 0.001). Our results showed that the coaxial vascular graft with the ratio of 0.2:0.11 had better mechanical properties (burst pressure reached 2844.55 ± 272.65 mmHg); Meanwhile its biological properties were also outstanding, which was beneficial to cell entry and offered good vascular remodeling performance. Polyurethane (PU); Gelatin (Gel); Polycaprolactone (PCL); polylactic acid (PLA);1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC); N-Hydroxy succinimide (NHS); 4-Morpholine-ethane-sulfonic (MES); phosphate buffered saline (PBS); fetal calf serum (FCS); Minimum Essential Medium (MEM); Dimethyl sulfoxide (DMSO); hematoxylineosin (HE).

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“…One of the main aims in vascular prosthesis research is therefore to develop an ideal vascular prosthesis claiming the possibility of sterilization, storage and rapid availability, no or at least low cytotoxicity, good handling in surgical processing, no induction of external body complaints, no foreign body reaction, low thrombogenicity, variability of caliber; wall thickness; and length, and stability with elasticity and porosity with sufficient tightness [ ]. Various surface coatings, for example, attempt to improve artificial vascular prosthesis material [ ], [ ], [ ], [ ]. Specifically, the direct and fastest possible cell colonization on the different coatings with endothelial cells was investigated in this context by many groups so far [ , , ], [ ], [ ].…”

Section: Introductionmentioning

confidence: 99%

“…Various surface coatings, for example, attempt to improve artificial vascular prosthesis material [ ], [ ], [ ], [ ]. Specifically, the direct and fastest possible cell colonization on the different coatings with endothelial cells was investigated in this context by many groups so far [ , , ], [ ], [ ]. In addition to the reduction of thrombogenicity, a fast endothelialization of the vascular prosthesis should reduce intimal hyperplasia and thus prevent stenoses.…”

Section: Introductionmentioning

confidence: 99%

Facilitation of adhesion and spreading of endothelial cells on silicone oxide-coated dacron material by microwave-excited low-pressure plasma

Tilkorn

Sorg

Sanders

et al. 2021

Innovative Surgical Sciences

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Objectives Autologous transplants are still the means of choice for bypass surgery. In addition to good tolerability, there is a reduced thrombogenicity and fewer neointima hyperplasia compared to artificial materials. However, since viable transplants are limited, attempts are being made to improve existing artificial vascular prosthesis material. Next to the reduction of thrombogenicity, a rapid endothelialization of the vascular graft should reduce intimal hyperplasia and thus prevent stenoses. The effect of newly developed silicon oxide coatings on the growth of endothelial cells was therefore the goal of this work in a cell culture study. Methods A woven, uncoated polyethylene terephthalate (PET) vessel prosthesis was used. The coating process was carried out in a low-pressure plasma reactor in a multi-step process. After preparation of the vacuum chamber hexamethyldisiloxane (HDMSO) with oxygen was evaporated using argon plasma. By this an approx. 1 nm thin adhesion promoter layer was separated from plasma and HMDSO. The silicone oxide barrier layer was applied to the PET vessel samples. The carbon content of the layer could be selectively altered by changing the HMDSO oxygen flow ratio, resulting in coatings of 100 nm, 500 nm, and 1,000 nm. In addition, two different oxygen-to-HMDSO ratios were used. To achieve a carbon coating as low as possible, the ratio was set to 200:1. A carbon-rich layer was obtained with the 1:1 setting. The various coatings were then examined for their surface texture by scanning electron microscopy (SEM) as well as by cell culture experiments for cell viability and growth using EA.hy 926 cells. Results SEM showed no changes in the surface morphology; however a layer thickness of 1,000 nm showed peeled off coating areas. Alamar blue assays showed a significantly higher metabolic activity (p=0.026) for the coating 500 nm, ratio 200:1 compared to untreated control samples and a significantly lower metabolic activity (p=0.037) of the coating 500 nm, ratio 1:1 compared to the coating 500 nm, ratio 200:1. This underlines the apparent tendency of the 1:1 coating to inhibit the metabolic activity of the cells, while the 200:1 coating increases the activity. Fluorescence microscopy after calcein acetoxymethyl ester (AM) staining showed no significant difference between the different coatings and the uncoated PET material. However, a tendency of the increased surface growth on the coating 500 nm, ratio 200:1, is shown. The coatings with the ratio 1:1 tend to be less densely covered. Conclusions The results of this work indicate a great potential in the silicon coating of vascular prosthesis material. The plasma coating can be carried out easy and gently. Cell culture experiments demonstrated a tendency towards better growth of the cells on the 200:1 ratio coating and a poorer growth on the carbon-rich coating 1:1 compared to the uncoated material. The coating with silicon oxide with a thickness of 500 nm and an oxygen-HMDSO ratio of 200:1, a particularly low-carbon layer, appears to be a coating, which should therefore be further investigated for its effects on thrombogenicity and intimal hyperplasia.

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Gelatin coating promotes in situ endothelialization of electrospun polycaprolactone vascular grafts

Cited by 23 publications

References 57 publications

Improving Biocompatibility of Polyester Fabrics through Polyurethane/Gelatin Complex Coating for Potential Vascular Application

Improving Biocompatibility of Polyester Fabrics through Polyurethane/Gelatin Complex Coating for Potential Vascular Application

Design and characterization of small-diameter tissue-engineered blood vessels constructed by electrospun polyurethane-core and gelatin-shell coaxial fiber

Facilitation of adhesion and spreading of endothelial cells on silicone oxide-coated dacron material by microwave-excited low-pressure plasma

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