Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Quan, Renfu; Zheng, Xuan; Xu, Shichao; Zhang, Liang; Yang, Disheng

doi:10.1186/scrt508

Cited by 45 publications

(24 citation statements)

References 48 publications

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“…Chondroitin sulfate, one of the major components of cartilage ECM, has important therapeutic properties, such as anti‐inflammatory effects while promoting wound healing by increasing cellular adhesion and proliferation during the healing process . To benefit from these properties, chondroitin sulfate has been combined with gelatin and HA for developing 3D scaffolds for cartilage tissue engineering and skin substitutes . Addition of chondroitin sulfate to gelatin improved the resistance against collagenase‐induced degradation, preserving the storage modulus and porosity of gelatin, while HA promoted the integration of engineered cartilage with the host tissue and improved the scaffold strength.…”

Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

292

210

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Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

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Section: Gelatin–polysaccharides Composites In Cell Culture and Tissumentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

292

210

View full text Add to dashboard Cite

show abstract

“…For instance, Perng CK et al cultured adult human bone marrow MSCs on gelatin scaffold with pNIPAAm as transplanted grafts for skin regeneration which could increase the re-epithelialization markers of human pan-cytokeratin and E-cadherin and decrease the stem cell markers of human CD13 and CD105 during the period of wound healing [59]. In another study, gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold was seeded with VEGF-165 modified hair follicle stem cells as a three-dimensional skin substitute [60]. Xu K et al utilized thiol-ene Michael-type addition for rapid encapsulation of MSCs within a gelatin/poly (ethylene glycol) biomatrix, which facilitated better function of MSCs in rat skin wound [61].…”

Section: Discussionmentioning

confidence: 98%

Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing

et al. 2015

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“…45 Previous studies showed that if integrated with other natural compounds, aminoacids, minerals, and various antioxidant compounds, it improves cutaneous aging-induced wrinkles 36 and stimulates hair follicles stem cells. 46 In fact, chondroitin sulfate binds and modulates the functions of a large number of biomolecules involved in hair follicle differentiation or proliferation, 47 including fibroblast growth factors (FGFs), 48 vascular endothelial growth factor (VEGF), 49 sonic hedgehog, 50 bone morphogenetic proteins (BMPs), 51 Wnt 50 and hepatocyte growth factor (HGF).…”

Section: Discussionmentioning

confidence: 99%

A dietary supplement improves age-related dermatological changes. Observational, anecdotal, spontaneous study.

et al. 2017

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Skin ageing involves also its annexes, such as hair and nails. Hair follicles are produced during embryogenesis. 1 In the postnatal life, hair follicle grows through three distinct phases: (i) anagen (growth) phase; (ii) catagen (regressing) phase; and (iii) telogen (resting) phase.2 This puzzling selfrenewal property is maintained by the stem cell niche located in the bulge portion of the hair follicle. 3 The hair follicle has a highly complex structure with eight distinct cell layers. The nail is hard keratin adnexa, produced by the same germinative epithelium and process through which epidermal skin cells produce the stratum corneum. Nail keratinocytes contribute to cornification through the production of hard keratins and the hardness of the nail plate is due to a high concentration of sulfur matrix protein. 4The rate of people with significant dermis and epidermis disorders increases linearly with age: at 70 years about 70% of the population claims at least one related-skin problem. 5The loss of hydration, appearance of fine wrinkles, roughness, dryness, thickening, laxity, telangiectasia, and loss of tensile strength are common features of the A B S T R A C T ORIGINAL ARTICLE ASIAN JOURNAL OF MEDICAL SCIENCESBackground: The rate of people with significant dermis and epidermis disorders increases linearly with age. The majority of skin changes are a consequence of cumulative exposure to environmental factors, such as chronic ultraviolet exposure. Aims and Objectives: Age-related dermatological changes include skin laxity, nails fragility, hair loss and hair breakage. This spontaneous, anecdotal observational study investigates if a dietary supplementation based on collagen, chondroitin sulfate, low-molecular weight hyaluronic acid, Pycnogenol® and coenzyme Q10 can improve age-related dermatological changes. Materials and Methods: Thirty four patients received the dietary supplement for 2 times/daily for 12 weeks. Facial skin hydration, Trans Epidermal Water Loss (TEWL) and elasticity were assessed at baseline and after 12 weeks in five selected areas. Nails hardness of the index, middle and ring fingers were also assessed at baseline and at followup by a durometer and by a nail score. The Hair Mass Index (HMI) and the Hair Breakage Index (HBI) were assessed by a mechanical device together with hair score at baseline and after 12 weeks. Results: After 12 weeks skin hydration, TEWL and elasticity significantly improved in all the tested areas (p < 0.01 and p < 0.001). Nails hardness of index, middle and ring finger significantly increased (p < 0.001) after 12 weeks. HMI and HBI were both significantly reduced (p < 0.0001) after 12 weeks. In addition, both nails and hair score improved. Conclusions: Dietary supplementation significantly improved age-related dermatological changes.

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Gelatin-chondroitin-6-sulfate-hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three-dimensional skin substitute

Cited by 45 publications

References 48 publications

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing

A dietary supplement improves age-related dermatological changes. Observational, anecdotal, spontaneous study.

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