Gelatin/Carboxymethyl chitosan based scaffolds for dermal tissue engineering applications

Agarwal, Tarun; Narayan, Rajan; Maji, Somnath; Behera, Shubhanath; Kulanthaivel, Senthilguru; Maiti, Tapas K.; Banerjee, Indranil; Pal, Kunal; Giri, Supratim

doi:10.1016/j.ijbiomac.2016.04.028

Cited by 111 publications

(67 citation statements)

References 25 publications

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“…But, the surface of the GA‐modified gelatin showed a porous network structure which was formed due to the sublimation of ice in the frozen gelatin samples generated during the freeze‐drying processing (Kang, Tabata, & Ikada, ). Therefore, GA‐modified gelatin was used to prepare porous gelatin scaffolds in the field of tissue engineering (Agarwal et al, ; Chaochai et al, ).…”

Section: Resultsmentioning

confidence: 99%

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Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Zhu

Zhang

et al. 2019

J Food Biochem

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Gelatin has been identified as a valuable material for controlled release of food ingredients because of its highly ramified three‐dimensional network. In the present work, gelatin was further modified with glutaraldehyde (GA) and prepared as microspheres to further improve its control to release of food ingredients. Features (i.e., chemical structures, thermal properties, network structures, and methylene blue (MB) release kinetics) of both the unmodified and GA‐modified gelatin microspheres were investigated. Results showed that the application of GA enhanced the formation of intramolecular and/or intermolecular crosslinking of gelatin and improved its thermal stability. Besides, the release efficiency of MB from the GA‐modified gelatin was less affected by temperature and pH value compared to the unmodified gelatin. Kinetics analysis proved that the release of MB from gelatin followed the first‐order kinetics. Both the release constant (k) of MB and the diffusion coefficient (Da) of GA‐modified gelatin were significantly lower than those of the unmodified gelatin. Practical applications Gelatin is a valuable material for the release control of food ingredients (e.g., oils, vitamins, antioxidants, and flavor compounds) attributes to its highly ramified three‐dimensional network. However, the low mechanical strength of gelatin makes it susceptible to many environmental factors and has restricted its application. In the present work, gelatin was modified by glutaraldehyde (GA). The chemical structure, thermal properties, network structure, and methylene blue release kinetics of the GA‐modified gelatin microspheres were properly investigated. This research proved that GA‐modified gelatin could be a potential carrier for the release control of given food contents in food industry.

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Section: Resultsmentioning

confidence: 99%

“…the freeze-drying processing (Kang, Tabata, & Ikada, 1999). Therefore, GA-modified gelatin was used to prepare porous gelatin scaffolds in the field of tissue engineering (Agarwal et al, 2016;Chaochai et al, 2016).…”

Section: Sem Analysismentioning

confidence: 99%

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Zhu

Zhang

et al. 2019

J Food Biochem

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“…6D. The collagenase enzymes are often used for evaluating the enzymatic degradation of wound dressings 66,67 . The PCL/Gel scaffolds which were immersed in the collagenase enzymes solution exhibited 46% and 78% weight loss after 7 and 14 days, respectively.…”

Section: Hydrolytic and Enzymatic Degradation Hydrolytic Degradationmentioning

confidence: 99%

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Eskandarinia

Kefayat

Agheb

et al. 2020

Sci Rep

133

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one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. in the present study, polycaprolactone/gelatin (pcL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (pU/eep). the pU/eep membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells' adhesion and proliferation. the bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers' diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats' skin wound model. taking together, the pU/eep-pcL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities. Skin is always at the exposer of different types of damages 1. Severe skin damages can be life-threatening due to loss of human body fluids, electrolytes, and nutritional components from the wound area. Therefore, wound dressings have gained lots of attention 2. An ideal wound dressing should protect the wound from external contaminants and facilitate the healing process. However, one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are

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“…[12,22]. Bununla birlikte yara örtücü olarak kullanılan kitosan filmlerinin su absorpsiyon kapasitelerinin %100-900 arasında değiştiği bilinmektedir [25,26]. Bu çalışmada hazırlanan sarı kantaron katkılı filmlerin su absorpsiyon kapasitelerinin %107-412 arasında değiştiği ve yara örtücü film olarak kullanılmaya uygun olduğu görülmektedir.…”

Section: Su İçeriği Ve Su Absorpsiyon Kapasitesi (Water Content and Wunclassified

Sarı Kantaron (Hypericum perforatum) katkılı kitosan ve jelatin esaslı yara örtücü filmlerinin üretimi ve karakterizasyonu

Küçük

Yildirim

2019

Gazi Üniversitesi Mühendislik Mimarlık Fakültesi Dergisi

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Highlights:Graphical/Tabular Abstract  Preparation of chitosangelatin based wound dressing film  Incoporation of St.John's Worth to wound dressing film  Antibacterial effect of St.John's Worth Figure A. Preparation scheme for St.john's wort supported wound dressing filmsPurpose: The present study aimed to investigate the potential use of St.John's Wort (Hypericum perforatum) incorporated films based on chitosan and gelatin as wound dressing. Theory and Methods:Wound infections due to various organisms often lead to challenging complications for public health during wound care management and have been conducted many studies to develop new wound dressing materials which plays critical role in the healing process . A new, innovative wound dressing material still needs to be developed to overcome the current limitations by accompanying the unique features, such as promoted moist environment, rapid wound healing, mechanical protection, non-cytotoxic to healthy tissue, antimicrobial/antifungal effects. In this work, St. John's Wort (Hypericum perforatum) supported wound dressing films based on chitosan and gelatin were prepared by using solvent casting technique. As wound dressing film structures were confirmed by FTIR and optical microscope its physical properties such as thickness, mass, water vapor permission rate (WVPR), water absorption capacity, water content and dressing pH were determined. Also, antibacterial activities were determined by the agar disc diffusion method for gram positive (S. Aureus, B. Subtilis) and gram negative (S. Typhi, E. Coli) microorganisms. Results:St.John's Wort (Hypericum perforatum) incorporated films that are investigated for their potential as wound dressing films have water absorption capacities of 107-646%, water contents of 6.4-23.1% and water vapor permeability rate (WVPR) of 356.25-984.38 g/m2day respectively. Also, pH values of films were range of 5.26-7.66 and these values are suitable for wound dressing application. It has been observed that all films exhibit antibacterial activity by forming inhibition diameters against four different microorganisms. Conclusion:Material selection is an important step to design an ideal wound dressing. St.John's Wort (Hypericum perforatum) incorporated films based on chitosan and gelatin were successfully prepared using a sequential solvent casting method. The results obtained in this study showed that St.John's Wort incorporated films could be used as potential candidates for wound healing application

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Gelatin/Carboxymethyl chitosan based scaffolds for dermal tissue engineering applications

Cited by 111 publications

References 25 publications

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Sarı Kantaron (Hypericum perforatum) katkılı kitosan ve jelatin esaslı yara örtücü filmlerinin üretimi ve karakterizasyonu

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