Gelatin and collagen binding to Staphylococcus aureus strains

Carret, G.; Emonard, H.; Fardel, G.; Druguet, M.; Herbage, D.

doi:10.1016/s0769-2609(85)80063-6

Cited by 17 publications

(11 citation statements)

References 9 publications

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“…It is also in accordance with that of Hazlett et al [25] and Ramphal et al [26] who reported that the receptors involved in the adherence of P. aeruginosa to bronchial epithelium is different from those involved in the adherence of the same pathogen to the corneal cells. Similar observations were previously reported with S. aureus [27,28], S. epidermidis [29,30], S. saprophyticus [24,31], and E. coli [32,33]. Accepting the idea that pathogens use different receptors to adhere to different types of mammalian cell will pave the road for accepting the conflict regarding antiadherent effect of carbocysteine with different cell lines.…”

Section: Discussionsupporting

confidence: 81%

Activity of some Mucolytics Against Bacterial Adherence to Mammalian Cells

Hafez

Aboulwafa

Yassien

et al. 2008

Appl Biochem Biotechnol

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In this article, some mucolytic agents were tested for their activity to prevent bacterial adherence to mammalian cells. Preliminary screening for antiadherent activity showed that ambroxol, bromhexine, ammonium chloride, and ammonium acetate but neither guaiphenesin nor carbocysteine significantly reduced the adherence of the tested clinical isolates to cultured mammalian cells. The antiadherent effect of such agents was observed when mammalian cells were treated with these agents either before or after bacterial adherence, and this effect was exhibited in a dose-dependent manner. The minimum concentrations of ambroxol, bromhexine, ammonium chloride, and ammonium acetate required for mammalian cells treatment to prevent bacterial adherence were 2.5, 5, 50, and 20 ng/ml, respectively, whereas significantly higher mucolytic concentrations were required to eradicate bacteria that adhered to mammalian cells. Upon treatment of mammalian cells with mucolytics, the maximum reduction in adherence of the tested isolates attained by ambroxol, bromhexine, ammonium chloride, ammonium acetate were 99%, 98%, 75%, and 54% to that of control, respectively. Insignificant difference existed between the antiadherent activities of ambroxol and bromhexine, while both agents had significantly higher effect than ammonium chloride and ammonium acetate. Pretreatment of the immobilized mucin with ambroxol, bromhexine, ammonium chloride, or ammonium acetate reduced the adherence of Pseudomonas aeruginosa, Escherichia coli, and staphylococcal isolates to this receptor analogue. A strong correlation was observed for the antiadherent activity of the tested mucolytics in case of mammalian cells and immobilized mucin. Moreover, pretreatment of the immobilized receptor analogues chondroitin sulfate-B and heparin with the abovementioned agents significantly reduced the adherence of Staphylococcus aureus, P. aeruginosa, and E. coli isolates to such immobilized glycoproteins.

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Section: Discussionsupporting

confidence: 81%

Activity of some Mucolytics Against Bacterial Adherence to Mammalian Cells

Hafez

Aboulwafa

Yassien

et al. 2008

Appl Biochem Biotechnol

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“…Previous studies have shown that bacteria may bind to fibronectin (7,15,23,25,26,32), laminin (7,16,27,29,32), and collagen (3,7,12,32) (5,23). These bacteria have been demonstrated to bind to the N-terminal region of fibronectin with high affinity (19).…”

mentioning

confidence: 99%

Binding of collagen to Staphylococcus aureus Cowan 1

Speziale¹,

Raucci²,

Visai³

et al. 1986

J Bacteriol

128

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(Pro-Gly-Pro)., (Pro-Pro-Gly)jo, and (Pro-OH-Pro-Gly)j0 were recognized by the receptor, whereas the peptides (Pro-Ala-Gly). and polyproline showed no inhibitory activity.The matrix proteins fibronectin and laminin may mediate the substrate adhesion of eucaryotic cells, and putative receptors present on the surfaces of cells are believed to recognize distinct sites in the adhesive proteins (35). Some eucaryotic cells (e.g., hepatocytes and chondrocytes) may also adhere to collagen substrate, and the presence of collagen-specific receptors has been implicated (18,22). The collagen receptor on hepatocytes appears to have broad specificity, since it binds to several different types of collagen as well as to isolated collagen a chains, collagen peptides, and synthetic collagen analogs (22).In the process of tissue adherence of pathogenic bacteria, structures at the host cell surface or in the extracellular matrix are recognized by specific receptors present on the bacterial cells. Previous studies have shown that bacteria may bind to fibronectin (7, 15, 23, 25, 26, 32), laminin (7, 16, 27, 29, 32), and collagen (3, 7, 12, 32) Chemicals. Type II collagen was purified from bovine nasal septum as described by Strawich and Nimmi (28). Native collagen types I and III were isolated with neutral salt from fetal bovine skin and purified by NaCl precipitation and DEAE chromatography (30). Type IV collagen was isolated from mouse tumor (31). Type V and VI collagens were purified from a pepsin digest of human placenta (1,20). The 1(I) and 2(I) chains both from calf and rat skin type I collagens were purified by carboxymethyl cellulose chromatography (21). The rat a chains were treated with cyanogen bromide, and the generated peptides (CB peptides) were purified as previously described (2, 6). The isolated peptides included CB2, CB5, CB6, CB7, and CB8 from the 1(I) chain and CB3, CB4, and CB5 from the a2 chain of rat type

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“…Moreover, a longer time to reach a binding equilibrium with 125I Type-I and II Cn suggests more Cn interacting sites on the cell surface of TSS than on non-TSS strains. Specific binding of different types of Cn to S. aureus and coagulase negative staphylococci has been described [21][22][23][24][25]. These observations are based on binding of soluble Cn to staphylococci.…”

Section: Discussionmentioning

confidence: 99%

Binding of type-I and type-II collagens toStaphylococcus aureusstrains isolated from patients with toxic shock syndrome compared to other staphylococcal infections

Naidu

Ekstrand

Wadström

1989

FEMS Microbiology Letters

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Toxic shock syndrome toxin‐1 (TSST‐1) producing strains of Staphylococcus aureus isolated from 18 patients with toxic shock syndrome (TSS) and from 56 patients with other diagnoses were compared for capacity to interact with various serum and connective tissue proteins. TSS associated isolates showed significantly stronger binding of Type‐I collagen (Cn‐I) and Cn‐II than non‐TSS strains, in a particle agglutination assay (PAA) as well as in 125I labelled Cn uptake experiments. 125I Cn‐IV binding, was similar between the two groups, whereas in PAA, a stronger interaction was observed for non‐TSS than TSS associated strains. The median binding of 125I Cn to TSS‐associated strains were 52.2 (Cn‐I), 30.6 (Cn‐II) and 20.0 (Cn‐IV) compared to 20.0 (Cn‐I), 14.4 (Cn‐II) and 24.4 (Cn‐IV) values of non‐TSS strains. A saturation with 125I Cn‐I and Cn‐II binding was established for TSS (30 min) and non‐TSS (15 min) strains. 125I Cn‐IV binding reached a saturation in 10 min and 90 min with TSS and non‐TSS strains respectively. Finally, the binding profiles of TSS associated and non‐TSS strains to fibronectin, fibrinogen, laminin and IgG did not differ in both PAA and radioisotope assays. In scanning electron microscopy, cells of TSS associated strains bound to the reprecipitated native Cn‐I fibrils. In contrast, most cells of non‐TSS strains were localized to the distal end or were trapped between the Cn fibrils. The stronger interaction with Cn‐I and II in particular, shown by TSS associated strains, might enhance submucosal localization, thereby facilitating entry of toxins into the blood and establishment of TSS.

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Gelatin and collagen binding to Staphylococcus aureus strains

Cited by 17 publications

References 9 publications

Activity of some Mucolytics Against Bacterial Adherence to Mammalian Cells

Activity of some Mucolytics Against Bacterial Adherence to Mammalian Cells

Binding of collagen to Staphylococcus aureus Cowan 1

Binding of type-I and type-II collagens toStaphylococcus aureusstrains isolated from patients with toxic shock syndrome compared to other staphylococcal infections

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