Gelam honey promotes ex vivo corneal fibroblasts wound healing

Yusof, Alia Md; Ghafar, Norzana Abd; Kamarudin, Taty Anna; Chua, Kien Hui; Azmi, Muhammad Fairuz; Ng, Sook-Luan; Yusof, Yasmin Anum Mohd

doi:10.1007/s10616-019-00349-8

Cited by 5 publications

(2 citation statements)

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“…The application of Gelam honey-based hydrogel decreased the inflammatory response as seen by the suppressed expression of proinflammatory cytokines IL-1α, IL-1β and IL-6 [ 128 ]. In another study, Gelam honey was shown to promote in vitro corneal fibroblast wound healing, where corneal fibroblast cultured with Gelam honey demonstrated faster wound closure than the control group [ 129 ]. Moreover, two studies have shown that Gelam honey at a concentration of 0.0015% promoted ex-vivo corneal keratocytes and corneal epithelial cell proliferation whilst preserving their phenotypical features [ 130 , 131 ].…”

Section: Medicinal Properties Of Tualang Gelam and Kelulut Honeysmentioning

confidence: 99%

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

et al. 2021

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Tualang, Gelam and Kelulut honeys are tropical rainforest honeys reported to have various medicinal properties. Studies related to the medicinal properties and physicochemical characteristics of these honeys are growing extensively and receiving increased attention. This review incorporated and analysed the findings on the biological and physicochemical properties of these honeys. Tualang, Gelam and Kelulut honeys were found to possess a wide variety of biological effects attributed to their physicochemical characteristics. Findings revealed that these honeys have anti-diabetic, anti-obesity, anti-cancer, anti-oxidative, anti-microbial, anti-inflammatory and wound-healing properties and effects on the cardiovascular system, nervous system and reproductive system. The physicochemical properties of these honeys were compared and discussed and results showed that they have high-quality contents and excellent antioxidant sources.

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Section: Medicinal Properties Of Tualang Gelam and Kelulut Honeysmentioning

confidence: 99%

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

et al. 2021

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“…Besides its high sugar content, honey also contains flavonoids, phenolic acid, and other minerals of different proportion, depending on the floral type, bee species, and geographical origin of the honey (Bogdanov et al., 2008; Buba et al., 2013; Cantarelli et al., 2008). Recently, several ex vivo studies have demonstrated positive effects of the different types of honey toward corneal wound healing and cell proliferation (Abd Ghafar et al., 2016; Yusof et al, 2016; Yusof et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

The role of Gelam honey in accelerating reepithelialization of ex vivo corneal abrasion model

et al. 2021

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This study aimed to investigate the role of Gelam honey (GH) in accelerating reepithelialization of corneal abrasion. Corneal epithelial cells (CEC) isolated from New Zealand white rabbit corneas, were cultured and circular-shaped wounds were created onto them, representing the corneal abrasion model. These wounds were treated with basal (BM) and cornea media (CM) supplemented with GH. The percentage of wound closure was measured on day 0, 3, and 5. Expressions of cytokeratin 3 (CK3), cluster of differentiation 44 (CD 44), and connexin 43 (Cx43) were analyzed via qRT-PCR and immunocytochemistry. The results showed CEC cultured in GHenriched media reepithelialized faster compared to control. Corneal abrasion treated with CM supplemented with GH closed completely on day 5. CK3, CD44, and Cx43 expressions correspond to the stages of reepithelialization. In conclusion, GH promotes the healing of the ex vivo corneal abrasion model. Further explorations of its potential as adjuvant therapy in treating corneal injuries are needed. Practical applicationsHoney has been reported to have many medicinal properties including antibacterial, anti-inflammatory, and the ability to promote skin wound healing. However, the effects of honey on corneal wound healing have not been fully elucidated. In the present study, we aimed to determine the effects of Gelam honey (GH), well-known local honey obtained from the beehive of Gelam trees (Melaleuca spp.), on the ex vivo corneal abrasion model via cell migration study and analysis of genes and proteins during corneal epithelial wound healing. GH has proven to have accelerated effects on the corneal epithelial cell migration during the closure of the ex vivo corneal abrasion wound model. The expressions of the genes and proteins of the corneal epithelial wound healing markers were in accordance with the stages of healing. Therefore, GH has the potential to be developed as adjuvant therapy in the form of GH-based eye drop in treating corneal injuries.

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