Gel Permeation Chromatography

Beri, Rajesh G.; Hacche, Laurel S.; Martin, C.

doi:10.1201/9781420042665.ch6

Cited by 3 publications

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“…Size-exclusion chromatography, also known as gel-permeation chromatography (GPC), is an analytical technique in which the separation of components in a reaction mixture is based on size rather than chemical affinity, as in HPLC. As such, GPC has been extensively used in the determination of polymer purity, dispersity, and molecular weight, as well as in the analysis of large biomolecules, such as proteins .…”

Section: Introductionmentioning

confidence: 99%

Characterization and Purification of Supramolecular Metal Complexes Using Gel-Permeation Chromatography

et al. 2004

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Gel-permeation chromatography (GPC) has been used to analyze transition-metal-based squares, triangles, and related supramolecular complexes. Using rhenium-containing molecular squares of different sizes, a linear calibration curve has been established, which was used for confirming the relative sizes of other assemblies. GPC can also discriminate cyclic trimers and tetramers of a dirhodium building block. Preparative GPC has been used to resolve macroscopic samples of a rhenium-based supramolecular mixture into pure syn and anti isomers. A mixture of "triangle" and "square" has also been successfully separated.

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Section: Introductionmentioning

confidence: 99%

Characterization and Purification of Supramolecular Metal Complexes Using Gel-Permeation Chromatography

et al. 2004

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“…Gel permeation chromatography (GPC) can give useful information on the average molecular weight distribution information for lignins and other polymers (Himmel et al, 1990). In GPC, there are no chemical interactions with a stationary or mobile phase; rather, analytes are separated based on their hydrodynamic volume (Beri et al, 2000). However, calibration is usually based on a polydisperse polymer such as polystyrene or poly(methyl methacrylate) and there are no specific standards yet available for lignin, making molar mass calculations questionable (Constant et al, 2016).…”

Section: Analytical Methods Without Mass Spectrometrymentioning

confidence: 99%

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Letourneau

Volmer

2021

Mass Spectrometry Reviews

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Lignin is currently one of the most promising biologically derived resources, due to its abundance and application in biofuels, materials and conversion to value aromatic chemicals. The need to better characterize and understand this complex biopolymer has led to the development of many different analytical approaches, several of which involve mass spectrometry and subsequent data analysis. This review surveys the most important analytical methods for lignin involving mass spectrometry, first looking at methods involving gas chromatography, liquid chromatography and then continuing with more contemporary methods such as matrix assisted laser desorption ionization and time-of-flight-secondary ion mass spectrometry. Following that will be techniques that directly ionize lignin mixtures-without chromatographic separation-using softer atmospheric ionization techniques that leave the lignin oligomers intact. Finally, ultra-high resolution mass analyzers such as FT-ICR have enabled lignin analysis without major sample preparation and chromatography steps. Concurrent with an increase in the resolution of mass spectrometers, there have been a wealth of complementary data analyses and visualization methods that have allowed researchers to probe deeper into the "lignome" than ever before. These approaches extract trends such as compound series and even important analytical information about lignin substructures without performing lignin degradation either chemically or during MS analysis. These innovative methods are paving the way for a more comprehensiveThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Chromatographic Separations of Enantiomers and Underivatized Oligosaccharides

Liu

2004

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Gel Permeation Chromatography

Cited by 3 publications

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Characterization and Purification of Supramolecular Metal Complexes Using Gel-Permeation Chromatography

Characterization and Purification of Supramolecular Metal Complexes Using Gel-Permeation Chromatography

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Chromatographic Separations of Enantiomers and Underivatized Oligosaccharides

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