Gel of Monascus purpureus JmbA rice accelerate wound healing in diabetic rats

Abstract

The most frequent complication of diabetes mellitus (DM) is diabetic ulcer, with a prevalence of 15-20% and an amputation rate of 30%. There is no effective and easy-to-apply treatment for acute-phase open wounds of patients with DM to prevent their development into ulcers. Monascus purpureus jmbA Rice (MJR) from Jember is superior to similar products from other regions in Indonesia because it has the highest monacolin content with an antioxidant effect. The study aimed to prove the effectiveness of MJR gel in accelerating the healing of acute wounds of diabetic rats. This true experimental laboratory research was a posttest-only control group design. We used Rattus norvegicus Wistar rats, 3-4 months old, weighing 200-300 grams. The 36 rats were divided into four treatment groups. The control group was given a gel of MJR 0%. The positive control group was given the antibiotic and placenta extract gel combination, and treatment groups T1 and T2 were given gel MJR40% and MJR80%. Excision wounds were made as deep as the subcutaneous layer on the back of the hyperglycemic rat. Four rats were terminated from each group on days 4, 11, and 18, and skin tissue was taken to observe the percentage of wound area shrinkage, epithelial thickness, and fibroblast count and analyze the levels of hydroxyproline and MDA. The MJR80% group had the highest percentage of wound area shrinkage, epithelial thickness, and fibroblast count compared to other groups. MDA levels decreased with increasing days in all treatment groups. Hydroxyproline levels increased on days 4 and 11, then decreased on day 18 in all treatment groups. We conclude that the 80% MJR group revealed the best acute diabetic wound healing. It was not statistically different from the combination of antibiotic and placenta extract gel treatment group.