Gel-Immobilized Heparin-Binding Lectin as Sensitive Sensor for Certain Groups of Charge-Bearing Carbohydrates

Koopmann, Jens-Oliver; Hocke, J.; Gabius, H.‐J.

doi:10.1515/bchm3.1993.374.7-12.1029

Cited by 8 publications

(7 citation statements)

References 9 publications

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“…Hydrogels are cross-linked polymeric networks that are swollen with solvent. There is a significant body of literature which has examined the swelling phenomenon of hydrogels. − ,− ,− These networks change volume as their environment changes. Electrically neutral hydrogels swell into good solvents, and this tendency to swell is resisted by the elastic restoring force of the hydrogel network, which arises from the crosslinks.…”

Section: Resultsmentioning

confidence: 99%

Intelligent Polymerized Crystalline Colloidal Arrays: Novel Chemical Sensor Materials

1998

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We report the development of a novel sensing material that reports on analyte concentrations via diffraction of visible light from a polymerized crystalline colloidal array (PCCA). The PCCA is a mesoscopically periodic crystalline colloidal array (CCA) of spherical polystyrene colloids polymerized within a thin, intelligent polymer hydrogel film. CCAs are brightly colored, and they efficiently diffract visible light meeting the Bragg condition. The intelligent hydrogel incorporates chemical molecular recognition agents that cause the gel to swell in response to the concentration of particular analytes; the gel volume is a function of the analyte concentration. The color diffracted from the hydrogel film is, thus, a function of analyte concentration: the swelling of the gel changes the periodicity of the CCA, which results in a shift in the diffracted wavelength. We have fabricated a sensor, utilizing a crown ether as the recognition agent, that detects Pb 2+ in the 0.1 µM-20mM (∼20 ppb-∼4000 ppm) concenration range. We have also fabricated glucose and galactose sensors, utilizing glucose oxidase or β-D-galactosidase as the recognition elements. The glucose oxidase sensor detects glucose in the 0.1-0.5 mM (18-90 ppm) concentration range in the presence of oxygen and detects as little as 10 -12 M glucose (0.18 ppt) in the absence of oxygen. In addition, this sensor reports on dissolved oxygen concentration from ∼1 to 6 ppm in the presence of constant glucose concentrations.

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Section: Resultsmentioning

confidence: 99%

Intelligent Polymerized Crystalline Colloidal Arrays: Novel Chemical Sensor Materials

1998

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“…These types of hydrogels are attracting more and more interest in biomedical applications because they exhibit a well-defined volume phase transition temperature (VPTT) in water, which is close to the body temperature. Hence, these gels can be expected to act as intelligent materials in controlled drug release [23,24], immobilization of enzymes and cells [25,26], separation of aqueous proteins and in tissue engineering [27].…”

Section: Reactive and Functional Polymersmentioning

confidence: 99%

Colloidal microgels as transdermal delivery systems

Lopez

Raghavan

Snowden

2004

Reactive and Functional Polymers

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“…Mechanical properties as well as the swelling and shrinking behavior of the gels change in response to physical or chemical stimuli, such as temperature, pH, ionic strength, solvent composition, and electric fields. Hence, these gels can be expected to act as intelligent materials in controlled or targeted drug release,2, 10–14 immobilization of enzymes and cells,15, 16 and in separation of aqueous proteins 16, 17…”

Section: Introductionmentioning

confidence: 99%

Effect of hydrophobicity of a drug on its release from hydrogels with different topological structures

Lowe

Tenhu

Tylli

1999

J. Appl. Polym. Sci.

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The effect of the topological structure; that is, the network heterogeneity, of hydrophobically modified, slightly acidic hydrogels on the binding and release of low molar mass drugs has been studied using ibuprofen and ephedrine as model compounds with varying water solubility. The difference in the heterogeneity of the gels has been produced by the choice of the hydrophobe copolymerized into the polymer network. The effect of the drug loading on the release kinetics has been investigated as well. The release of hydrophobic ibuprofen was slower from a strongly aggregated heterogeneous gel than from a more homogeneous one, because of the strong hydrophobic interaction between ibuprofen and the heterogeneous hydrogel. The release of hydrophilic ephedrine from the homogeneous gel with an initial drug content of 30 wt % of dry polymer showed negative time dependence, indicating that during and after the swelling of the gel, ephedrine started to bind to the polymer. However, the release of ephedrine from a heterogeneous hydrogel increased with time. This shows that the heterogeneous, aggregated polymer binds the hydrophobic substance more strongly than the homogeneous one does, and that the homogeneous network has higher affinity for the basic hydrophilic substance than the heterogeneous one does. The loading contents of ibuprofen and ephedrine affect the release rates in different ways because of the different binding and release mechanisms. The number of binding sites accessible for ephedrine inside the polymer network is assumed to change upon the swelling of the gel.

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Gel-Immobilized Heparin-Binding Lectin as Sensitive Sensor for Certain Groups of Charge-Bearing Carbohydrates

Cited by 8 publications

References 9 publications

Intelligent Polymerized Crystalline Colloidal Arrays: Novel Chemical Sensor Materials

Intelligent Polymerized Crystalline Colloidal Arrays: Novel Chemical Sensor Materials

Colloidal microgels as transdermal delivery systems

Effect of hydrophobicity of a drug on its release from hydrogels with different topological structures

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