Gel green fluorescence ssDNA aptasensor based on carbon nanotubes for detection of anthrax protective antigen

Karimi, Farrokh; Dabbagh, Somayyeh

doi:10.1016/j.ijbiomac.2019.08.219

Cited by 20 publications

(8 citation statements)

References 46 publications

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“…In another study, a colorimetric assay that utilized asymmetric PCR amplicons/AuNPs complexes was able to detect B. anthracis at 10 pg/ml detection limit with no cross-reactivity ( 50 ). Likewise, a multi-walled carbon nanotube (MWCNTs)-based fluorescence aptasensor was reported to detect the recombinant protective antigen domain 4 (rPAD4) of B. anthracis within 10 min ( 51 ). The immobilized aptamer was labeled with Gel Green, and a sensitivity of 20 ng/ml and 62.5 ng/ml purified and unpurified rPAD4 protein, respectively, was reported ( 51 ).…”

Section: Molecular-based Methodsmentioning

confidence: 99%

“…Likewise, a multi-walled carbon nanotube (MWCNTs)-based fluorescence aptasensor was reported to detect the recombinant protective antigen domain 4 (rPAD4) of B. anthracis within 10 min ( 51 ). The immobilized aptamer was labeled with Gel Green, and a sensitivity of 20 ng/ml and 62.5 ng/ml purified and unpurified rPAD4 protein, respectively, was reported ( 51 ). When coupled together, nanomaterials and aptamers can reduce cost as well as the turn-around time while increasing the sensitivity of the assay ( 50 , 51 ).…”

Section: Molecular-based Methodsmentioning

confidence: 99%

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Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Manhas

Quintela

2021

Front. Vet. Sci.

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Nanotechnology has gained prominence over the recent years in multiple research and application fields, including infectious diseases in healthcare, agriculture, and veterinary science. It remains an attractive and viable option for preventing, diagnosing, and treating diseases in animals and humans. The apparent efficiency of nanomaterials is due to their unique physicochemical properties and biocompatibility. With the persistence of pathogens and toxins in the poultry and livestock industries, rapid diagnostic tools are of utmost importance. Though there are many promising nanomaterials-based diagnostic tests specific to animal disease-causing agents, many have not achieved balanced sensitivity, specificity, reproducibility, and cost-effectiveness. This mini-review explores several types of nanomaterials, which provided enhancement on the sensitivity and specificity of recently reported diagnostic tools related to animal diseases. Recommendations are also provided to facilitate more targeted animal populations into the development of future diagnostic tools specifically for emerging and re-emerging animal diseases posing zoonotic risks.

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Section: Molecular-based Methodsmentioning

confidence: 99%

Section: Molecular-based Methodsmentioning

confidence: 99%

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Manhas

Quintela

2021

Front. Vet. Sci.

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“…This group is shared by seven species, including B. cereus, B. thuringiensis, B. mycoides, B. pseudomycoides, B. cytotoxicus, B. toyonensis, and B. weihenstephanensis. The mentioned species are divergent, phenotypically and pathogenically [42,43]. Bacillus anthracis is an etiological agent of anthrax, a zoonotic-currently a rare disease in humans that is associated with potential bioterror use, and considered as a serious biological threat.…”

Section: Bacillus Anthracismentioning

confidence: 99%

Dangerous Pathogens as a Potential Problem for Public Health

et al. 2020

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Pathogens are various organisms, such as viruses, bacteria, fungi, and protozoa, which can cause severe illnesses to their hosts. Throughout history, pathogens have accompanied human populations and caused various epidemics. One of the most significant outbreaks was the Black Death, which occurred in the 14th century and caused the death of one-third of Europe’s population. Pathogens have also been studied for their use as biological warfare agents by the former Soviet Union, Japan, and the USA. Among bacteria and viruses, there are high priority agents that have a significant impact on public health. Bacillus anthracis, Francisella tularensis, Yersinia pestis, Variola virus, Filoviruses (Ebola, Marburg), Arenoviruses (Lassa), and influenza viruses are included in this group of agents. Outbreaks and infections caused by them might result in social disruption and panic, which is why special operations are needed for public health preparedness. Antibiotic-resistant bacteria that significantly impede treatment and recovery of patients are also valid threats. Furthermore, recent events related to the massive spread of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are an example of how virus-induced diseases cannot be ignored. The impact of outbreaks, such as SARS-CoV-2, have had far-reaching consequences beyond public health. The economic losses due to lockdowns are difficult to estimate, but it would take years to restore countries to pre-outbreak status. For countries affected by the 2019 coronavirus disease (COVID-19), their health systems have been overwhelmed, resulting in an increase in the mortality rate caused by diseases or injuries. Furthermore, outbreaks, such as SARS-CoV-2, will induce serious, wide-ranging (and possibly long-lasting) psychological problems among, not only health workers, but ordinary citizens (this is due to isolation, quarantine, etc.). The aim of this paper is to present the most dangerous pathogens, as well as general characterizations, mechanisms of action, and treatments.

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“…Magnetic field is used to isolate complex and the oligonucleotides are quantified in the form of magnitude of reduction peak current using square wave voltammetry [ 87 ]. Interaction of domain 4 protective antigen of Bacillus anthracis with gel green–labeled aptamer-MWCNTs induces decrease in fluorescence intensity [ 93 ]. DNA sequence that codes for protein responsible for pathogenesis or drug resistance can be explored to develop nanosensor for unexplored mutant, drug-resistant, and genetically modified pathogens.…”

Section: Detection Of Gmo Mutated and Resistant Organismsmentioning

confidence: 99%

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Kumar

Guleria

2020

Curr Pollution Rep

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Gel green fluorescence ssDNA aptasensor based on carbon nanotubes for detection of anthrax protective antigen

Cited by 20 publications

References 46 publications

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Enhanced Detection of Major Pathogens and Toxins in Poultry and Livestock With Zoonotic Risks Using Nanomaterials-Based Diagnostics

Dangerous Pathogens as a Potential Problem for Public Health

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

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