Gel-forming mucin interactome drives mucus viscoelasticity

Demouveaux, Bastien; Gouyer, Valérie; Gottrand, Frédéric; Narita, Tetsuharu; Desseyn, Jean‐Luc

doi:10.1016/j.cis.2017.12.005

Cited by 83 publications

(109 citation statements)

References 209 publications

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“…Generally, TFF2 would be ideally designed to modulate the packaging and oligomerization of MUC6 along the secretory pathway and the viscoelastic properties of gastric mucus . The latter might be relevant for the development of new drugs . Here, TFF2 could eventually support the release of MUC6 oligomers and stabilize, for example, a two‐ or three‐dimensional network.…”

Section: Resultsmentioning

confidence: 99%

“…Here, TFF2 could eventually support the release of MUC6 oligomers and stabilize, for example, a two‐ or three‐dimensional network. Different release mechanisms have already been described for the mucins MUC2, MUC5AC, and MUC5B, the structures of which are similar to that of MUC6 . The topological organization of MUC6 oligomers is probably of importance for the permeability of gastric mucus.…”

Section: Resultsmentioning

confidence: 99%

“…[35,36] The latter might be relevant for the development of new drugs. [56] Here, TFF2 could eventually support the release of MUC6 oligomers and stabilize, for example, at wo-or three-dimensional network. Different release mechanisms have already been described for the mucinsM UC2, MUC5AC, and MUC5B,t he structures of which are similar to that of MUC6.…”

Section: Resultsmentioning

confidence: 99%

“…Different release mechanisms have already been described for the mucinsM UC2, MUC5AC, and MUC5B,t he structures of which are similar to that of MUC6. [55][56][57][58] The topological organizationo fM UC6 oligomers is probably of importance for the permeability of gastric mucus. This might also influence the absorption of nutrients and drug delivery.…”

Section: Resultsmentioning

confidence: 99%

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Commercial Porcine Gastric Mucin Preparations, also Used as Artificial Saliva, are a Rich Source for the Lectin TFF2: In Vitro Binding Studies

et al. 2018

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Mucous gels (mucus) cover internal body surfaces. The secretory mucins MUC5AC and MUC6 and the protective peptide TFF2 are characteristic constituents of gastric mucus; TFF2 is co‐secreted with MUC6. Herein, we investigated two commercial mucin preparations by FPLC and proteomics, because they are model systems for studying the rheology of gastric mucins. One preparation is also used as a saliva substitute, for example, after radiation therapy. We show that both preparations contain TFF2 (≈0.6 to 1.1 %, w/w). The majority of TFF2 is strongly bound noncovalently to mucin in a manner that is resistant to boiling in SDS. First overlay assays with 125I‐labeled porcine TFF2 revealed that mucin binding is modulated by Ca2+ and can be blocked by the lectin GSA‐II and the antibody HIK1083, both recognizing the peripheral GlcNAcα1→4Galβ1→R moiety of MUC6. TFF2 binding was also inhibited in the presence of Me‐β‐Gal but less so by the α anomer. TFF2 may play a role in the oligomerization and secretion of MUC6, the rheology of gastric mucus, and the adherence of gastric microbiota. TFF2 in artificial saliva may be of benefit. TFF2 might also interact with the sugar moiety of various receptors.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Commercial Porcine Gastric Mucin Preparations, also Used as Artificial Saliva, are a Rich Source for the Lectin TFF2: In Vitro Binding Studies

et al. 2018

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“…A vital feature of this mucin family is the occurrence of several O-glycans attached to the PTS domain, specifically to the threonine and serine residues (Backstrom et al 2013). The gel-forming mucins are distinguished by the presence of other remarkable domains like von Willebrand factor (vWF) and cysteine (CYS) domains (Bonser and Erle 2017;Demouveaux et al 2018). The CYS domain is a region of 110 amino acids containing 10 invariant cysteine residues close to the PTS domain, which is thought to be important in the protein network (Verdugo 2012).…”

Section: Gel-forming Mucinsmentioning

confidence: 99%

Mucin 2 (MUC2) promoter characterization: an overview

Yamashita

Melo

2018

Cell Tissue Res

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Transgenic livestock have been studied with a well-known interest in improving quantitative and qualitative traits. In order to direct heterologous gene expression, it is indispensable to identify and characterize a promoter suitable for directing the expression of the gene of interest (GOI) in a tissue-specific way. The gastrointestinal tract is a desirable target for gene expression in several mammalian models. Throughout the surface of the intestinal epithelium, there is an intricate polymer network, formed by gel-forming mucins (especially MUC2 and MUC5AC, of which MUC2 is the major one), which plays a protective role due to the formation of a physical, chemical and immunological barrier between the organism and the environment. The characterization of the gel-forming mucins is difficult because of their large size and repetitive DNA sequences and domains. The main mucin in the small and large intestine, mucin 2 (MUC2), is expressed specifically in goblet cells. MUC2 plays an important role in intestinal homeostasis and its disruption is associated with several diseases and carcinomas. This mucin is also an important marker for elucidating mechanisms that regulate differentiation of the secretory cell lineage. This review presents the state of the art of MUC2 promoter structure and functional characterization.

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The filter‐house of the larvaceanOikopleura dioica. A complex extracellular architecture: From fiber production to rudimentary state to inflated house

Razghandi

Janßen

et al. 2021

Journal of Morphology

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While cellulose is the most abundant macromolecule in the biosphere, most animals are unable to produce cellulose with the exception of tunicates. Some tunicates have evolved the ability to secrete a complex house containing cellulosic fibers, yet little is known about the early stages of the house building process.Here, we investigate the rudimentary house of Oikopleura dioica for the first time using complementary light and electron microscopic techniques. In addition, we digitally modeled the arrangement of chambers, nets, and filters of the functional, expanded house in three dimensions based on life-video-imaging. Combining 3Dreconstructions based on serial histological semithin-sections, confocal laser scanning microscopy, transmission electron microscopy, scanning electron microscopy (SEM), and focused ion beam (FIB)-SEM, we were able to elucidate the arrangement of structural components, including cellulosic fibers, of the rudimentary house with a focus on the food concentration filter. We developed a model for the arrangement of folded structures in the house rudiment and show it is a precisely preformed structure with identifiable components intricately correlated with specific cells. Moreover, we demonstrate that structural details of the apical surfaces of Nasse cells provide the exact locations and shapes to produce the fibers of the house and interact among each other, with Giant Fol cells, and with the fibers to arrange them in the precise positions necessary for expansion of the house rudiment into the functional state. The presented data and hypotheses advance our knowledge about the interrelation of structure and function on different biological levels and prompt investigations into this astonishing biological object.

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Gel-forming mucin interactome drives mucus viscoelasticity

Cited by 83 publications

References 209 publications

Commercial Porcine Gastric Mucin Preparations, also Used as Artificial Saliva, are a Rich Source for the Lectin TFF2: In Vitro Binding Studies

Commercial Porcine Gastric Mucin Preparations, also Used as Artificial Saliva, are a Rich Source for the Lectin TFF2: In Vitro Binding Studies

Mucin 2 (MUC2) promoter characterization: an overview

The filter‐house of the larvaceanOikopleura dioica. A complex extracellular architecture: From fiber production to rudimentary state to inflated house

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