Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions

McGookin, R.

doi:10.1385/0-89603-064-4:93

Cited by 1 publication

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characteristics Source or reference* Forrai et al, 1983 Quality and concentration of the RNA was analyzed in agarose gels according to the method of McGookin (1984b) and by spectrophotometric measurement.…”

Section: Strainsmentioning

confidence: 99%

“…RNA was phenol extracted, ethanol precipitated, treated with DNAse (Worthington Biochemical, Freehold, N J), and reprecipitated (Maniatis et al, 1982) to yield 20-30 0,g RNA from 40 to 50 mg nodules. Quality and concentration of the RNA was analyzed in agarose gels according to the method of McGookin (1984b) and by spectrophotometric measurement.…”

Section: Rna Isolationmentioning

confidence: 99%

See 1 more Smart Citation

Rhizobium fix genes mediate at least two communication steps in symbiotic nodule development.

Putnoky

Grosskopf

et al. 1988

The Journal of Cell Biology

View full text Add to dashboard Cite

Abstract.To identify bacterial genes involved in symbiotic nodule development, ineffective nodules of alfalfa (Medicago sativa) induced by 64 different Fixmutants of Rhizobium meliloti were characterized by assaying for symbiotic gene expression and by morphological studies. The expression of leghemoglobin and nodulin-25 genes from alfalfa and of the nifHD genes from R. meliloti were monitored by hybridizing the appropriate DNA probes to RNA samples prepared from nodules. The mutants were accordingly divided into three groups. In group I none of the genes were expressed, in group II only the plant genes were expressed and in group III all three genes were transcribed. Light and electron microscopical analysis of nodules revealed that nodule development was halted at different stages in nodules induced by different group I mutants. In most cases nodules were empty lacking infection threads and bacteroids or nodules contained infection threads and a few released bacteroids. In nodules induced by a third mutant class bacteria were released into the host cells, however the formation of the peribacteroid membrane was not normal. On this basis we suggest that peribacteroid membrane formation precedes leghemoglobin and nodulin-25 induction, moreover, after induction of nodulation by the nod genes at least two communication steps between the bacteria and the host plants are necessary for the development of the mature nodule. By complementing each mutant of group I with a genomic R. meliloti library made in pLAFR1, four new fix loci were identified, indicating that several bacterial genes are involved in late nodule development.HIZOBIA are able to cooperate with leguminous plants to fix atmospheric nitrogen. Nitrogen fixation takes place in symbiotic nodules, new plant organs developed for this purpose. Nodule cells harbor unique cell organelles, bacteroids, derived from Rhizobium bacteria which reduce dinitrogen to ammonia. The differentiation and function of nodules involves symbiosis-specific expression of both bacterial and plant genes (nodulin genes) in a highly coordinated manner, as well as communication between the two partners. This communication process seems to consist of signal exchange during nodule development. The molecular basis of these events are, at present, mostly unknown.Recently, plant flavones were reported to act as signal molecules inducing the expression of bacterial nodulation (nod)

show abstract

Section: Strainsmentioning

confidence: 99%

Section: Rna Isolationmentioning

confidence: 99%