Gel chromatography of heparin

Losito, R; Gattiker, H; Bilodeau, Ginette

doi:10.1016/s0378-4347(00)84206-1

Cited by 17 publications

(6 citation statements)

References 24 publications

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“…Many methods, such as ion exchange, gel or affinity chromatography, and countercurrent distribution, can be used to separate the various molecular components of heparin. 17,28,36,40,47,51,52,64,70 The pharmacology of heparin is made more complex by the fact that each of these molecular components exhibits varying interactions with endogenous modulating proteins and sites. Thus, being a polycomponent drug with multicomponent endogenous interactions and transformations, heparin represents a challenge to biochemists and pharmacologists involved in exploring the mechanism of action and therapeutic effect of this drug.…”

Section: Fig 1 Diagramatic Illustration Of the Heterogeneous Naturementioning

confidence: 99%

Heparin, Its Fractions, Fragments and Derivatives Some Newer Perspectives

Fareed¹

1985

Semin Thromb Hemost

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A brief attempt has been made to provide an overview of the field of heparinology. As stated, in coming years one should witness significant developments in this area. It is, however, important to caution that the newer products derived from heparin may not behave like heparin, and one must consider their individual molecular and biochemical interactions. The available limited data on heparin may or may not be applicable to the newer heparin fractions or derivatives. Independent preclinical and clinical trials are needed to investigate the pharmacology and therapeutic actions of newer heparin derivatives.

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Section: Fig 1 Diagramatic Illustration Of the Heterogeneous Naturementioning

confidence: 99%

Heparin, Its Fractions, Fragments and Derivatives Some Newer Perspectives

Fareed¹

1985

Semin Thromb Hemost

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“…We also found 28 that heparin could be excreted into the bile by the isolated perfused liver, supporting the hypothesis of hepatic involvement in the regulation of the ultimate pharmacologic effects of heparin. It was shown 29 that column chromatography on G-50 Sepharose could resolve pork mucosal heparin into three components and that only the larger molecular weight fraction possessed anticoagulant activity in vivo. Widlund and coworkers 51 found that both in the rat and the dog heparin fragments were excreted more rapidly into the urine when compared with routine heparin; after 72 hours, the liver retained most of the labeled heparin.…”

Section: Metabolismmentioning

confidence: 99%

Molecular Weight of Heparin Versus Biologic Activity Some Additional Considerations

Losito¹,

Losito²

1985

Semin Thromb Hemost

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“…The production process involves a proteolytic digestion, followed by extraction and combination with ion pairing reagents and, finally, fractional precipitation and several purification steps based on anion exchange processes [5,6]. The biological activity of its commercial preparations depends on purity, molecular mass distribution and degree of sulfation, as well as on the presence of specific oligosaccharide sequences which occur differently in heparins from different sources [7,8,9]. All these factors must be controlled in order to obtain the appropriate anticoagulant activity while, at the same time, minimizing the bleeding side effect.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the composition of heparin mixtures from various origins using proton nuclear magnetic resonance and multivariate calibration methods

et al. 2002

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A multivariate calibration method for the characterization of heparin samples based on the analysis of (1)H nuclear magnetic resonance (NMR) spectral data is proposed. Heparin samples under study consisted of two-component or four-component mixtures of heparins from porcine, ovine and bovine mucosae and bovine lung. Although the (1)H NMR spectra of all heparin types were highly overlapping, each origin showed some particular features that could be advantageously used for the quantification of the components. These features mainly concerned the anomeric H, which appeared in the range 5.0-5.7 ppm and the peaks of acetamidomethyl protons at 2.0-2.1 ppm. The determination of the percentage of each heparin class depended on these differences and was carried out using partial least squares regression (PLS) as a calibration method. Prior to the PLS analysis, the spectral data were standardized using the internal standard peak (sodium 4,4-dimethyl-4-silapentanoate- 2,2,3,3- d (4), TSP) as the reference. The quantification of each heparin type in the samples using PLS models built with 4 or 5 components was satisfactory, with an overall prediction error ranging from 3% to 10%.

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Gel chromatography of heparin

Cited by 17 publications

References 24 publications

Heparin, Its Fractions, Fragments and Derivatives Some Newer Perspectives

Heparin, Its Fractions, Fragments and Derivatives Some Newer Perspectives

Molecular Weight of Heparin Versus Biologic Activity Some Additional Considerations

Estimation of the composition of heparin mixtures from various origins using proton nuclear magnetic resonance and multivariate calibration methods

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