Gebe Konya Merinosu Koyunlarında Erken Gebelikte Gebelik İlişkili Glikoproteinlerin Plazma Profilinin Belirlenmesi

Kaplan, Yasemin; Özyurtlu, Nihat; Köse, Mehmet; Atlı, Mehmet Osman; Küçükaslan, İbrahim; Kırbaş, Mesut

doi:10.17094/ataunivbd.588666

Cited by 3 publications

(9 citation statements)

References 7 publications

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“…Furthermore, sensitivities similar to the sensitivity determined for the rapid visual PAG ELISA in the present study were reported for the "Idexx visual pregnancy test" in Santa Ines ewes 30 days after laparoscopic insemination (Chaves et al, 2017) and for the "Idexx bovine pregnancy test" in Corriedela and Ile de France x Texel sheep throughout pregnancy (Rovani et al, 2016). On the other hand, Kaplan et al (2019) reported a low sensitivity for the "Idexx bovine pregnancy test" before the 56th day of pregnancy in Konya Merino sheep. In addition, Chaves et al (2017) determined a lower sensitivity for the "Idexx visual pregnancy test" on day 26 post-mating compared to day 28 post-mating (73.17 versus 92.68, respectively).…”

Section: Resultssupporting

confidence: 84%

“…PAG ELISA pregnancy tests commercially validated for cattle have been used in various Brasilian and European sheep breeds (Rovani et al, 2016;Chaves et al, 2017;Steckeler et al, 2018). It is stated that PAG profiles in the peripheral blood can vary between sheep breeds; therefore, determining the specific PAG profile of breeds native to Turkey is important for improved reproductive management (Kaplan et al, 2019). The aim of this study was to evaluate the diagnostic reliability of a bovine rapid visual PAG ELISA test and transabdominal ultrasonography in standing position for early pregnancy diagnosis in Awassi sheep using lambing results as a gold standard.…”

Section: Introductionmentioning

confidence: 99%

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İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Akköse¹

2020

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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The aim of this study was to evaluate two different methods (a bovine rapid visual PAG ELISA test and transabdominal ultrasonography) for early diagnosis of pregnancy in Awassi sheep. The ewes used in this study (n=50) were hand-mated in June of 2019 and lambed in October of 2019. Pregnancy diagnosis was performed 34-38 days after mating by rapid visual PAG ELISA test and transabdominal ultrasonography in standing position. The diagnostic test characteristics of both methods were calculated using lambing results as a gold standard. According to the lambing results, it was determined that, of the 50 ewes, 42 (84%) were pregnant and 8 (16%) were not. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of rapid visual PAG ELISA and transabdominal ultrasonography were 97.6%, 62.5%, 93.2%, 83.3%, 92% and 95.2%, 100%, 100%, 80%, 96%, respectively. In McNemar's analysis, no difference was found between the pregnancy diagnosis methods and lambing results. In conclusion, both rapid visual PAG ELISA and transabdominal ultrasonography in standing position proved to be reliable and practical methods for pregnancy diagnosis as of day 34 post-mating in Awassi ewes.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Akköse¹

2020

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…PAGs are specific proteins that aid in pregnancy diagnosis and small ruminant management. However, different results have been reported for PAGs levels, which show volatility during the early gestation period (El Amiri et al, 2014Kaplan et al, 2018;Ranilla et al, 1994). Previous studies also suggest that differences in PAGs or PSPB levels could be associated with the sheep breed (Redden & Passavant, 2013;Ranilla et al, 1994).…”

Section: Discussionmentioning

confidence: 95%

“…Serum Kaplan et al, 2018;Ranilla et al, 1994) for results pertaining to early gestation are attributed to the genetic variations of breeds.…”

Section: Discussionmentioning

confidence: 99%

Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Akköse,

Çınar,

Yazlık

et al. 2024

Veterinary Medicine & Sci

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BackgroundReported as being expressed by mono‐ and binucleate placental cells, pregnancy‐associated glycoproteins (PAGs) are released into the blood circulation from the ruminant placenta. Circulating gestational PAGs levels may differ between sheep breeds.ObjectiveThis study was aimed at the close monitoring of the serum PAGs profiles of Karya and Konya Merino sheep during early pregnancy.MethodsFifteen Karya and 15 Konya Merino ewes were synchronized by a 12‐day treatment with progesterone‐impregnated intravaginal sponges. After the sponges were withdrawn, the ewes were administered 400 IU of equine chorionic gonadotropin. The ewes were allowed to mate naturally, and all animals were sampled for blood as of the day of mating (day 0) at weeks 1, 2, 3, 4 and 5. Pregnancy diagnoses were made by transabdominal ultrasonography at week 5. At weeks 6, 7, 8, 9 and 11, blood samples were collected only from the pregnant ewes. The blood samples were centrifuged at 3000 × g, and extracted sera were stored at −20°C until being used for laboratory analyses. Serum PAGs levels were determined with the aid of a commercial PAG‐enzyme‐linked immunosorbent assay test originally developed for pregnancy diagnosis in cattle. Differences in the between the PAGs levels throughout pregnancy and the group effect (Karya and Konya Merino) were determined with a two‐way mixed analysis of variance. Pairwise comparisons were made using a Bonferroni adjustment.ResultsPAGs levels showed a linear increase with the advance of pregnancy in both Karya and Konya Merino sheep. No difference was detected between the breeds for serum PAGs levels. The serum PAGs levels of the pregnant and non‐pregnant ewes differed as of the fourth week.ConclusionThe serum PAGs levels of the Karya and Konya Merino ewes were similar during the first 11 weeks of gestation, and pregnancy diagnosis could be made based on serum PAGs levels as of the 4th week in both breeds.

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“…However, with the progression of implantation and placentation, they reach a much higher concentration than can be detected in maternal circulation on day 35. Moreover, the PAGs level in the maternal blood reaches the highest level at the end of the pregnancy due to the increase in the functional mass of the placenta [9,10,11]. It is stated that the migration of binuclear trophoblastic cells to maternal tissue and their association with endometrial cells further facilitate the detection and passage of PAGs into the maternal circulation [3].…”

Section: Introducti̇onmentioning

confidence: 99%

Plasma profile of Pregnancy Associated Glycoproteins during Postpartum period in Konya Merino ewes lambing single and twin

Ucar,

Kose,

Kirbas

et al. 2023

RC FCV-LUZ

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This study investigated the plasma profile of Pregnancy Associated Glycoproteins (PAGs) after lambing in Konya Merino ewes using specific cattle pregnancy test kit. A total of 16 Konya Merino ewes were used as a material. Four groups were set up, ewes birthing a male lamb (SM group, n=4), a female lamb (SF group, n=4), twin male lambs (TM group, n=4) or twin female lambs (TF group, n=4). Blood plasma samples were taken on days 0 (lambing day), 3, 7, 10, 14 and then weekly until day 35. All samples were analysed using a bovine pregnancy test kit to detect of PAGs level. It was determined that there was a strong negative correlation between PAGs levels in peripheral blood and days after lambing (r2=0.969; P<0.01). However, no relationship was found between PAGs level and lamb gender or birth type. In conclusion, plasma PAGs level decreases rapidly in Konya Merino ewes regardless of lamb gender and birth type. Moreover, the results showed that the plasma profile of PAGs after lambing in Konya Merino ewes can be monitored with a bovine commercial ELISA–based pregnancy test kit, and the test results can be used in decisions and assessments based on the levels of PAG molecules.

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Gebe Konya Merinosu Koyunlarında Erken Gebelikte Gebelik İlişkili Glikoproteinlerin Plazma Profilinin Belirlenmesi

Cited by 3 publications

References 7 publications

İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Plasma profile of Pregnancy Associated Glycoproteins during Postpartum period in Konya Merino ewes lambing single and twin

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