GDSL lipases modulate immunity through lipid homeostasis in rice

Gao, Ming‐Jun; Yin, Xin; Yang, Weibing; Lam, Sin Man; Tong, Xiaohong; Liu, Jiyun; Wang, Xin; Li, Qun; Shui, Guanghou; He, Zuhua

doi:10.1371/journal.ppat.1006724

Cited by 110 publications

(103 citation statements)

References 66 publications

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“…6). Previous studies have shown that miR156 silencing confers enhanced resistance to BPH [30], and GDSL lipases modulate immunity through lipid homeostasis [45]. Therefore, miR156b-3p may negatively regulate BPH resistance by targeting GDSL lipases.…”

Section: Discussionmentioning

confidence: 96%

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

Tan

Guo

et al. 2020

BMC Genomics

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Background: The brown planthopper (BPH, Nilaparvata lugens Stål) is a kind of phloem-feeding pest that adversely affects rice yield. Recently, the BPH-resistance gene, BPH6, was cloned and applied in rice breeding to effectively control BPH. However, the molecular mechanisms underlying BPH6 are poorly understood.Results: Here, an integrated miRNA and mRNA expression profiling analysis was performed on BPH6-transgenic (BPH6G) and Nipponbare (wild type, WT) plants after BPH infestation, and a total of 217 differentially expressed miRNAs (DEMs) and 7874 differentially expressed mRNAs (DEGs) were identified. 29 miRNAs, including members of miR160, miR166 and miR169 family were opposite expressed during early or late feeding stages between the two varieties, whilst 9 miRNAs were specifically expressed in BPH6G plants, suggesting involvement of these miRNAs in BPH6-mediated resistance to BPH. In the transcriptome analysis, 949 DEGs were opposite expressed during early or late feeding stages of the two genotypes, which were enriched in metabolic processes, cellular development, cell wall organization, cellular component movement and hormone transport, and certain primary and secondary metabolite synthesis. 24 genes were further selected as candidates for BPH resistance. Integrated analysis of the DEMs and DEGs showed that 34 miRNAs corresponding to 42 target genes were candidate miRNA-mRNA pairs for BPH resistance, 18 pairs were verified by qRT-PCR, and two pairs were confirmed by in vivo analysis. Conclusions:For the first time, we reported integrated small RNA and transcriptome sequencing to illustrate resistance mechanisms against BPH in rice. Our results provide a valuable resource to ascertain changes in BPHinduced miRNA and mRNA expression profiles and enable to comprehend plant-insect interactions and find a way for efficient insect control.

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Section: Discussionmentioning

confidence: 96%

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

Tan

Guo

et al. 2020

BMC Genomics

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“…Lipid content was referenced with deacylated anionic phospholipids used as standards. Other glycerolipids were extracted and analysed as described previously (Gao et al, ; Sun et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Other glycerolipids were extracted and analysed as described previously (Gao et al, 2017;Sun et al, 2016).…”

Section: Lipid Profiling and Ip 3 Measurementmentioning

confidence: 99%

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

Deng

Yuan

Cao

et al. 2018

Plant Cell & Environment

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Phosphatidylinositol-specific phospholipase C (PI-PLC) is involved in stress signalling but its signalling function remains largely unknown in crop plants. Here, we report that the PI-PLC4 from rice (Oryza sativa cv), OsPLC4, plays a positive role in osmotic stress response. Two independent knockout mutants, plc4-1 and plc4-2, exhibited decreased seedling growth and survival rate whereas overexpression of OsPLC4 improved survival rate under high salinity and water deficiency, compared with wild type (WT). OsPLC4 hydrolyses PI, phosphatidylinositol 4-phosphate (PI4P), and phosphatidylinositol-4,5-bisphosphate (PIP ) to generate diacylglycerol (DAG) in vitro. Knockout of OsPLC4 attenuated salt-induced increase of phosphatidic acid (PA) whereas overexpression of OsPLC4 decreased the level of PI4P and PIP under salt treatment. Applications of DAG or PA restored the growth defect of plc4-1 to WT but DAG kinase inhibitor 1 blocked the complementary effect of DAG in plc4-1 under salt stress. In addition, the loss of OsPLC4 compromised the increase of inositol triphosphate and free cytoplasmic Ca ([Ca ] ) and inhibited the induction of genes involved in Ca sensor and osmotic stress response to salt stress. The results indicate that OsPLC4 modulates the activity of two signalling pathways, PA and Ca , to affect rice seedling response to osmotic stress.

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“…The secretion of GDSL lipases was sensitive to disruption in either SYP121 or SYP122 (and, therefore, likely independent of SYP132). GDSL lipases have broad substrate and functional specificities and have been associated with plant immunity (Kim et al, 2013;Gao et al, 2017).…”

Section: Members Of Multigene Families Are Trafficked By Different Romentioning

confidence: 99%

SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

et al. 2018

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A.M.E.J.).SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion and contribute to homoeostasis, pathogen defense, cell expansion, and growth in plants. In Arabidopsis (Arabidopsis thaliana), two homologous Qa-SNAREs, SYNTAXIN OF PLANTS121 (SYP121) and SYP122, facilitate the majority of secretory traffic to the plasma membrane, and the single mutants are indistinguishable from wild-type plants in the absence of stress, implying a redundancy in their functions. Nonetheless, several studies suggest differences among the secretory cargo of these SNAREs. To address this issue, we conducted an analysis of the proteins secreted by cultured wildtype, syp121, and syp122 mutant Arabidopsis seedlings. Here, we report that a number of cargo proteins were associated differentially with traffic mediated by SYP121 and SYP122. The data also indicated important overlaps between the SNAREs. Therefore, we conclude that the two Qa-SNAREs mediate distinct but complementary secretory pathways during vegetative plant growth.

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GDSL lipases modulate immunity through lipid homeostasis in rice

Cited by 110 publications

References 66 publications

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

Phosphatidylinositol‐hydrolyzing phospholipase C4 modulates rice response to salt and drought

SNAREs SYP121 and SYP122 Mediate the Secretion of Distinct Cargo Subsets

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