Gdf5 is expressed in the developing skeleton of median fins of late-stage zebrafish, Danio rerio

Crotwell, Patricia L.; Clark, Tim G.; Mabee, Paula M.

doi:10.1007/s00427-001-0186-z

Cited by 25 publications

(37 citation statements)

References 14 publications

(13 reference statements)

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“…4G). The expression around the endoskeletal radials is consistent with published data (Crotwell et al, 2001). Antisense RNA probes were prepared using the following templates: gdf5 (Clement et al, 2008); evx1 (Thaeron et al, 2000).…”

Section: In Situ Hybridizationsupporting

confidence: 71%

“…GDF5 is required for the formation of at least some tetrapod limb joints Kingsley, 1996, 1999;Francis-West et al, 1999;Settle et al, 2003;Masuya et al, 2007) and consistent with the above-mentioned homology, gdf5 expression can be observed in endoskeleton joints in the fins during zebrafish larval development (Crotwell et al, 2001; Fig. 4G).…”

Section: Developmental Dynamicssupporting

confidence: 60%

“…Most notably, the former progress through a cartilaginous stage whereas the latter do not (Geraudie and Landis, 1982;Landis and Geraudie, 1990;Smith, 1994;Hinchliffe, 2002;Suzuki et al, 2003;Mari-Beffa et al, 2007). The separation of proximal and distal radials in the endoskeleton of these fins is thought to be homologous to joint formation in amniote limbs (where the bones also progress through a cartilaginous stage) and similar factors are expressed during both of these processes (Crotwell et al, 2001;Crotwell and Mabee, 2007). However, before our analysis of the evx1 mutant, it was not clear whether this genetic homology also extended to the lepidotrichia joints.…”

Section: Introductionmentioning

confidence: 99%

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Evx1 is required for joint formation in zebrafish fin dermoskeleton

Schulte

Allen

England

et al. 2011

Developmental Dynamics

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The transcription factor Evx1 is expressed in the joints between individual lepidotrichia (bony ray) segments and at the distal tips of the lepidotrichia in developing zebrafish fins. It is also expressed in the apical growth zone in regenerating fins. However, so far there is no functional evidence that addresses whether Evx1 is required for any aspect of fin development or regeneration. In this study, we use a novel mutation in evx1 to address this. We find that Evx1 is not required for either fin outgrowth or regeneration. All of the fins form normally in evx1 mutants, and there are no significant changes in fin length. In contrast, Evx1 is required for lepidotrichia joint formation during both fin development and regeneration. This is a very specific phenotype as both lepidotrichia hemisegment separations and lepidotrichia bifurcations still form normally in evx1 mutant fins, as do joints in the more proximal endoskeletal radials. Developmental Dynamics 240:1240-1248,

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Section: In Situ Hybridizationsupporting

confidence: 71%

Section: Developmental Dynamicssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

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Evx1 is required for joint formation in zebrafish fin dermoskeleton

Schulte

Allen

England

et al. 2011

Developmental Dynamics

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“…Lending support to this hypothesis, in the zebrafish, it was found that the dynamic expression patterns of the genes involved during fin formation (e.g., Gdf5,bmp2a,bmp2b) are similar for both the dorsal and anal fins and are associated with the bidirectional radial development patterns observed in both of these fins (Crotwell et al 2001(Crotwell et al , 2004. The integrated development of the endo-and exoskeletal components of the median fins is also thought to be regulated by a developmental module (Mabee et al 2002).…”

Section: Introductionmentioning

confidence: 84%

Head, Body and Fins: Patterns of Morphological Integration and Modularity in Fishes

2015

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Actinopterygians demonstrate high levels of morphological disparity, especially in the variation of fin positions, sizes and shapes. One hypothesis to explain the diversity of fin morphologies is that it is facilitated by a modular organization. According to this hypothesis, fin modules would be quasi-independent during ontogeny or evolution, facilitating their evolvability. We investigated variational modularity of fins in two cyprinid species, the zebrafish (Danio rerio) and the Northern redbelly dace (Chrosomus eos), to determine which subsets of fins are quasi-independent and which are most highly integrated in positioning. Hypotheses of modularity were evaluated using a combination of methods suitable for analyses of landmarks. The hypothesis that the dorsal and anal fins belong to a posterior trunk and tail module is strongly supported, a finding that can be explained by the use of subcarangiform locomotion in these two species. There is also some support for the hypothesis that the paired fins and head region each constitute variational modules. The support for fin variational modules is weaker than expected considering the wealth of developmental evidence supporting fin modularity. This might be related to a dissociation of the fin positioning modules during actinopterygian evolution, a process that had already been suggested for the dorsal and anal fins. Alternatively, the fin modules inferred from developmental data might not directly translate into variational modules: variational modules can incorporate the signals from numerous partially overlapping developmental processes so that one to one correspondence between developmental and variational modules is not always expected.

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“…Among these are studies discussing the role of chordin in skeletal development (Fisher and Halpern, 1999), dlg3 in vertebral ankylosis (Kö nig et al, 1999), Gdf5 in median fins (Crotwell et al, 2001), sox9 in cartilage differentiation (Chiang et al, 2001), shh and bmp2b in the caudal fin (Quint et al, 2002), and endothelin-1 and pbx4 in the larval head (Kimmel et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Developmental morphology of the axial skeleton of the zebrafish,Danio rerio(Ostariophysi: Cyprinidae)

Bird

Mabee

2003

Developmental Dynamics

315

427

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Before our rapidly increasing knowledge of gene interactions can be connected with the morphologic defects in mutant zebrafish, the normal course of skeletal development must be understood. Here, we describe the developmental morphology of the axial skeleton of zebrafish and review it in relation to the morphology of related species. The relative sequence of ossification in the skeleton is described. Two separate centers of development were found in the axial skeleton (Weberian apparatus and caudal fin) in contrast to tetrapods, which have a single anterior center. Slight variation was found in the overall relative timing of development. The extensive ichthyological literature on teleost anatomy and recent genetic data form the basis for our review and interpretation of homologies of various elements of the axial skeleton. Because homology forms the basis for all evolutionary comparisons, these data are critical for integration in evo-devo studies. Developmental Dynamics 228:337-357, 2003.

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Gdf5 is expressed in the developing skeleton of median fins of late-stage zebrafish, Danio rerio

Cited by 25 publications

References 14 publications

Evx1 is required for joint formation in zebrafish fin dermoskeleton

Evx1 is required for joint formation in zebrafish fin dermoskeleton

Head, Body and Fins: Patterns of Morphological Integration and Modularity in Fishes

Developmental morphology of the axial skeleton of the zebrafish,Danio rerio(Ostariophysi: Cyprinidae)

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