GCMS, phytochemicals and antioxidant activities of in vitro callus extracts of Strobilanthes kunthiana (Nees) T. Anderson ex Benth: An endemic plant of Acanthaceae

Abstract

Strobilanthes kunthiana (Neelakurinji) is an endemic and underexploited plant belongs to the Family Acanthaceae. The aim of our study was to evaluate phytochemical analysis, GC-MS analysis and antioxidant activity of in vitro callus extract of Strobilanthes kunthiana. In this present study the phytochemical analysis of various extract of S. kunthiana in vitro callus were studied. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, steroids, terpenoids and phenols in the methanolic extract comparing to the other extracts. In this study GC-MS analysis revealed the presence of 10 bioactive phytochemical compounds were identified in the methanolic extract. The prevailing compounds were 9,12-octadecadienoic acid (Z,Z) (50.32%), hexadecanoic acid, methyl ester (20.69%), 9-octadecenoic acid (Z)-,methyl ester (10.45%), heptadecanoic acid, 16-methyl-, methyl ester (5.78%), 2,6-bis(1,1-dimethylethyl)-4-methyl phenol (4.70%). 3-methyl-2-ketobutyric acid tbdms (2.79%), 2,2,3,4-tetramethyl-5-hexen-3-ol (2.41%), N-(tertbutoxycarbonyl)-2-(4-methoxyphenyl) allylamine (1.06%), cyclotrisiloxane, hexamethyl (0.94%), benzenesulfonamide (0.87%). The antioxidant property was evaluated for methanol and ethanolic extract by DPPH method. The higher percentage of inhibition (79.23 ± 0.37) was observed in 250 µg/mL of ethanol extract followed by (90.35 ± 0.54) methanolic extract against the standard ascorbic acid (91.25 ± 0.33). The results show that the methanolic extract possesses more antioxidant activity than ethanol. The plant S. kunthiana may be exploited as a source of natural antioxidant and as herbal alternatives for various disorders.

Protective Efficacy of Two Endemic Strobilanthes of Southern Western Ghats Kerala

“…Since these two species were analyzed for the first time, the high protective efficacy was in consistent with the previously reported species of the genus [14,15] . Strobilanthes has been suggested as a herbal alternative after proper analysis of antioxidants in it [16] . The herbal tea from dried leaves increased the defense system and reduced the blood glucose level [17,18] .…”

A Mechanistic Study of Analgesic and Anticancer Activity of unexplored plant Strobilanthes Kunthiana Phytocosntituents using Molecular Docking Studies.

“…The principle compounds were lupeol, Betulin, 9,12-octadecadienoic acid, hexadecenoic acid, methyl ester, 9-octadecenoic acid, methyl ester, alpha-amyrin, β-sitosterol, 4-amino-tetrahydro-2H-pyran-3,5-diol, Decahydro-1,1,4a,8-tetramethyl phenanthren-2 (1H,3H,4Bh)-one, (Dheptadecanoic acid, 16-methyl-methyl ester, 2,6-bis (1,1-dimethylethyl0-4-methylphenol, 3-methyl-2-ketobutyric acid, 2,2,3,4-tetramethyl-5-hexen-3-ol, N-(tertbutoxycarbonyl)-2(-methoxyphenyl) allylamine, cyclotrisiloxane, hexamethyl, benzene sulfonamide (Fig. 3) [4]. Whereas, phytoconstitiutents lupeol and Botulin were found in larger concentration, Hence, this research approaches to target cancer because cancer is one of the leading causes of death worldwide, and the pain associated with it is very intense.…”

“…The Western Ghats valleys, where this unusual plant was found, are a popular destination for tourists [3]. S. kunthiana leaf callus extract in vitro provides a crucial component for pharmacological activity [4]. Acute respiratory in ammation, gastrointestinal disorders, rheumatism, anxiolytic, anti-diabetic, laxative, anticancer, diuretic, anti-arthritic, and anti-in ammatory activities [5].…”

Isolation and Structural Characterization of Phytoconstituents from Strobilanthes kunthianus ArticleView

“…8 Everlyne et al 9 studied FT-IR and GC-MS analysis of ethanolic leaves extract of S. kunthianus and reported that it may contains aromatic hydrocarbons, straight chain primary alcohols, fatty acids, alkenes, phenolic compounds, titerpene alcohols and triterpenes, primary aliphatic alcohols and trienoic fatty acids. Prabakaran and Kirutheka 10 identified ten phytoconstituents based on GC-MS analysis in callus methanolic extract of S. kunthianus and studied for antioxidant properties. However, so far there is no phytoconstituents are isolated individually in various plant parts of S. kunthianus.…”