GCF levels of human beta defensins 1and 3 in diabetic and non diabetic patients with chronic periodontitis

Abstract

Background: Human beta defensins (HβDs) expression in healthy tissues may aid in preventing the onset and/or progression of inflammatory disease.Type 2 diabetes mellitus (type 2 DM) and periodontal disease encompass inflammatory changes. HβDs in periodontitis patients is differentially regulated by periodontal pathogens, commensal organisms, host derived factors and inflammatory mediators. Additionally hyperglycaemia might influence this inflammatory state. Aim: To evaluate the role of the expression of human beta-defensin 1 and 3 levels in gingival crevicular fluid samples of gingivitis and chronic periodontitis patients with various glycaemic status. Methods and Material: In this study a total number of 140 patients were divided into five groups (Group I-V) consisted of 28 patients under each group. Group I consisted of 28 Type 2 Diabetes mellitus (DM) patients with well controlled glycemic status (> 6.5 <7%HbA1C) and chronic periodontitis(CP),Group II compromised of 28 type 2 DM-CP patients(7-8% HbA1C),Group III had 28 Type 2 DM-CP patients (>8%HbA1C).The concentrations of HβD-1 and HβD-3 were estimated using ELISA. Results: HβD-1 and 3 levels are higher in gingivitis subjects than periodontitis. Significantly higher levels of HβD-3 expression were found in the healthy tissues compared to the diseased ones.