GC/TOF-MS-based phytochemical analysis, in vitro antiproliferative effects, antioxidant and antibacterial activity of Sarcophyte sanguinea subsp. piriei (Hutch.) B. Hansen

Mbakazi, Y.; Kappo, A.P.; Soyingbe, Oluwagbemiga Sewanu; Nety, N.S.; Makhafola, Tshepiso Jan; Chukwuma, Chika I.; Dikhoba, M.P.; Mariri, N.G.; Mongalo, N.I.

doi:10.1016/j.sajb.2022.08.003

Cited by 3 publications

(13 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In comparing the phytocompounds from three extracts, viz acetone, methanol, and ethyl acetate extracts, which exhibited potent biological activity, only an organoheterosilane compound known as 2-(2’,4’,4’,6’,6’,8’,8’-Heptamethyltetrasiloxan-2’-yloxy)-2,4,4,6,6,8,8,10,10-decamethylcyclopentasiloxane was detected in all extracts ( Table 4 ). A similar compound was earlier identified from another medicinal plant extract with potent antibacterial activity [ 15 , 70 ]. Although the biological activity of the compound is not well documented, the organoheterosilanes are well known for their potential antimicrobial and anti-inflammatory activity [ 71 , 72 ].…”

Section: Discussionmentioning

confidence: 64%

“…These may well suggest that only the ethyl acetate extract satisfies that criterion, hence, worth introspecting for further analysis. In the current work, the extracts with LC 50 values ranging from >20 μg/mL to 45 μg/mL are moderately exhibiting some cytotoxic effect or anticancer activity against the tested cell line of choice, while extracts exhibiting IC 50 > 45 are inactive [ 15 ]. According to Mongalo and Makhafola [ 7 ], some traditional healers within the Blouberg area, Limpopo Province, South Africa, topically apply the powdered plant material directly to the wound or the infected breast to treat and manage breast cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Cancer is an illness that results from the development of tumor cells surpassing the growth of normal human cells and may well infect important human body organs that include the breast, liver, stomach, cervix, brain, blood, and prostate [ 15 ]. The disease may be genetically inherited and is more prevalent in Europe than in any other continent.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

Mongalo

Raletsena

Munyai

2023

Life

Self Cite

View full text Add to dashboard Cite

Cissus cornifolia (Baker) Planch is traditionally used in South African traditional medicine (SATM) to treat a variety of human infections. The antimicrobial activity of extracts from C. cornifolia was investigated in vitro against a plethora of pathogenic microorganisms using the microdilution assay. The acetone extract exhibited a notable minimum inhibitory concentration (MIC) value of 0.10 mg/mL against Mycoplasma hominis and a further MIC of 0.20 mg/mL against Candida parapsilosis, Streptococcus agalactiae, Pseudomonas aeruginosa, and Enterococcus faecalis. In the antiproliferative assays, both the ethyl acetate and methanol extracts exhibited a potent inhibition of the MCF-7-21 cell line. In the anti-inflammatory assays, both the ethyl acetate and methanol extracts exhibited IC50 values of 15.59 and 15.78 µg/mL against Cyclooxygenase-2 (COX-2), respectively. Methanol extract further exhibited potent dual inhibition of both COX-2 and 15-LOX enzymes, hence, recommended to curb both related cancers, particularly breast cancer and inflammation-borne diseases. In the comparative gas chromatography time-of-flight mass spectrometry (GC/TOF-MS), the acetone, ethyl acetate, and methanol extract contained significantly prevalent amounts of compound 2-(2’,4’,4’,6’,6’,8’,8’-Heptamethyltetrasiloxan-2’-yloxy)-2,4,4,6,6,8,8,10,10-nonamethylcyclopentasiloxane with % area ranging from 15.714 to 39.225. The findings in the current work validates the use of the plant species in SATM in the treatment of cancer-like infections, opportunistic infections associated with HIV-AIDS. Furthermore, the in vivo studies and the mechanisms of action still need to be explored.

show abstract

Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

Mongalo

Raletsena

Munyai

2023

Life

Self Cite

View full text Add to dashboard Cite

show abstract

“…The in vitro antioxidant activities of the aqueous and organic extracts of S. piriei were determined using various biochemical assay methods. Available data show that these extracts are potent scavengers of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with IC 50 of 4.26 ± 0.22 μg/mL (methanol extract) and 4.62 ± 0.14 μg/mL (dichloromethane extract) compared with ascorbic acid (IC 50 = 1.09 ± 0.01 μg/mL) and butylated hydroxytoluene (BHT) (IC 50 = 3.96 μg/mL) ( Mbakazi et al, 2022 ).…”

Section: Phytochemistry Of Sarcophyte Sparrmmentioning

confidence: 99%

“…In the 2 -2′-Azino-di-[3-ethylbenzthiazoline sulfonate (ABTS) assay, the methanolic and dichloromethane extracts of S. piriei had IC 50 values of 15.52 ± 0.12 μg/mL and 5.62 ± 0.05 μg/mL, while ascorbic acid and BHT had IC 50 values of 1.71 ± 0.00 μg/mL and 3.61 ± 0.08 μg/mL, respectively ( Mbakazi et al, 2022 ). In addition, these extracts demonstrated considerable iron chelating effects with IC 50 values of 1.82 ± 0.01 μg/mL and 3.50 ± 0.09 μg/mL for the methanolic and dichloromethane extracts, respectively, against the standards (ascorbic acid = 0.096 ± 0.04 μg/mL; BHT = 1.00 ± 0.01 μg/mL) ( Mbakazi et al, 2022 ).…”

Section: Phytochemistry Of Sarcophyte Sparrmmentioning

confidence: 99%

Ethnomedicinal uses, phytochemistry, and pharmacology of the genus Sarcophyte: a review

Jared Misonge,

Gervason Apiri,

James Onsinyo

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Although medicinal plants have been used by ethnic communities since ancient times to prevent and treat various diseases, only a few have been scientifically documented. Therefore, due to their rare availability and lack of comprehensive scientific information, we reviewed the ethnomedicinal uses, phytochemistry, and pharmacological activities of plants within the genus Sarcophyte. To do this, we used specific search terms and phrases to retrieve relevant information from online sources published in English from 2000 to July 2023. The results showed that there are only two plants in the genus Sarcophyte (Sarcophyte sanguinea Sparrm. and Sarcophyte piriei Hutch.), which are traditionally used to treat a wide range of diseases, especially cancer, and skin, gastrointestinal, and urinogenital tract ailments in humans, and to cure animals in ethnoveterinary practices. It was noted that 13 secondary metabolites have been isolated from the two plants, the most prominent of which are flavonoids (diinsininol, diinsinin, and naringenin). The antioxidant activity of S. piriei is reported based on the scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50: 4.26 ± 0.22 μg/mL) and 2 -2′-Azino-di-[3-ethylbenzthiazoline sulfonate (ABTS) radicals (IC50: 4.62 ± 0.14 μg/mL), chelating iron (IC50: 1.82 ± 0.01 μg/mL, 3.50 ± 0.09 μg/mL), and nitric oxide (IC50: 9.97 ± 0.88 μg/mL, 9.09 ± 0.11 μg/mL). The methanolic stem extracts of S. piriei possess antimicrobial activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Vibrio fluvialis, and Enterococcus avium, with minimum inhibitory concentration (MIC) values ranging from 0.16 to 0.625 mg/mL, and a minimum bactericidal concentration (MBC) of 1.25 to 5 mg/mL. Cytotoxic effects of the extracts from the two plant species were also demonstrated. Sarcophyte piriei possesses therapeutic potential as evidenced by the inhibitory effects of the aqueous rhizome extract on edema (1,000 mg/kg) and prostaglandin synthesis (IC50 = 0.2 mg/mL). In addition, diinsininol and diinsinin were isolated from S. sanguinea inhibited prostaglandin synthesis (IC50: 9.20 µM, 13.14 µM) and platelet-activating factor-induced exocytosis. Therefore, based on this review, further scientific research is needed to demystify the links between traditional medicinal uses, various secondary metabolites, and the pharmacology of the two plants.

show abstract

A review of phylogeny, medicinal values, phytochemistry and toxicity of Sarcophyte piriei Hutch (Balanophoraceae)

Dennis¹,

Timothy²,

Joseph³

2023

Afr. J. Pharm. Pharmacol.

View full text Add to dashboard Cite

In recent years, plants have shown potential as sources of antimicrobial agents due to their immense phytochemical constituents, and there is a need to continuously search and develop potent antimicrobial agents to combat the growing threat of antimicrobial resistance worldwide. The challenges facing the utilization of ethnomedicine are lack of sufficient studies to ascertain their therapeutic use. Sarcophyte is a holoparasite plant and trophic guild parasite. Its native ranges from Southern parts of Ethiopia to South Africa. It has a vital medicinal value in managing various disorders. This review article purposes to offer a complete rundown literature regarding Sarcophyte genus: Phylogeny, medicinal values, phytochemistry and toxicity that have been outlined in diverse journals and articles. This review has been developed with the aid of books, articles, peer reviewed articles, theses, Google Scholar, Science Direct and validated internet sources. Sarcophyte piriei has been utilized customarily to manage a variety of disorders among Africans for ages. It is employed to treat diseases but is not limited to sores, bruises, sore throat, swollen glands, toothache, abdominal pain, diarrhoea, shingles, cancer, snake bites and menstrual disorders. Alkaloids, flavonoids, phenols, saponins, terpenoids and tannins have shown to be the major phytochemical constituents correlated with the antimicrobial properties of the holoparasite and contributed to the antibacterial prospects of the plant against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia and Proteus mirabilis. Its safety and toxicity have not been well established hence the need for evaluation. More studies are required to elucidate more phytochemical composition, medicinal values, efficacy, potency, safety and toxicity measures. Sarcophyte genus will provide a novelty in new remedies for the emerging antimicrobial resistance to conventional antimicrobial agents.

show abstract

GC/TOF-MS-based phytochemical analysis, in vitro antiproliferative effects, antioxidant and antibacterial activity of Sarcophyte sanguinea subsp. piriei (Hutch.) B. Hansen

Cited by 3 publications

References 42 publications

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

Ethnomedicinal uses, phytochemistry, and pharmacology of the genus Sarcophyte: a review

A review of phylogeny, medicinal values, phytochemistry and toxicity of Sarcophyte piriei Hutch (Balanophoraceae)

Contact Info

Product

Resources

About