GC–MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

Romoli, Riccardo; Papaleo, Maria Cristiana; Pascale, Donatella de; Tutino, Maria Luisa; Michaud, Luigi; LoGiudice, A.; Fani, Renato; Bartolucci, Gianluca

doi:10.1007/s11306-013-0549-2

Cited by 47 publications

(29 citation statements)

References 20 publications

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“…These properties facilitate evaporation and diffusion through both water-and gas-filled pores in soil and rhizosphere environments. Hence, microbial volatiles have important roles in marine and terrestrial environments (Schulz et al, 2010;Romoli et al, 2014). To date, the chemical structure of~1000 volatiles have been described originating from a wide range of bacterial and fungal genera and species (Effmert et al, 2012;Lemfack et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The amount and composition of volatiles produced by microorganisms can vary according to culturing conditions (Claeson, 2007;Blom et al, 2011;Garbeva et al, 2014a,b). Other important factors influencing the production of volatiles are the physiological state of the producing microorganism, oxygen availability, moisture, temperature and pH (Insam and Seewald, 2010;Romoli et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Volatile affairs in microbial interactions

et al. 2015

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Microorganisms are important factors in shaping our environment. One key characteristic that has been neglected for a long time is the ability of microorganisms to release chemically diverse volatile compounds. At present, it is clear that the blend of volatiles released by microorganisms can be very complex and often includes many unknown compounds for which the chemical structures remain to be elucidated. The biggest challenge now is to unravel the biological and ecological functions of these microbial volatiles. There is increasing evidence that microbial volatiles can act as infochemicals in interactions among microbes and between microbes and their eukaryotic hosts. Here, we review and discuss recent advances in understanding the natural roles of volatiles in microbe-microbe interactions. Specific emphasis will be given to the antimicrobial activities of microbial volatiles and their effects on bacterial quorum sensing, motility, gene expression and antibiotic resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Volatile affairs in microbial interactions

et al. 2015

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“…Similarly, the selective inhibition of the P. nodorum VOC complement against bacteria observed is not without precedent. It has been previously demonstrated that compounds produced by sponge-associated Arctic microbial communities show a strong inhibitory activity against the opportunistic pathogenic bacterial Burkholderia cepacia complex but not to other pathogenic bacteria 23,24 . Why VOCs from P. nodorum would harbour this specificity is unclear but it has been suggested that such selectivity may be a reflection of how different organisms respond differently to the same chemical cue or alternatively it may be a consequence on possible fitness differences among individuals.…”

Section: Discussionmentioning

confidence: 99%

Volatile molecules secreted by the wheat pathogenParastagonospora nodorumare involved in development and phytotoxicity

Gonzalez

Yap

Breen

et al. 2019

Preprint

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AbstractSeptoria nodorum blotch is a major disease of wheat caused by the fungus Parastagonospora nodorum. Recent studies have demonstrated that secondary metabolites, including polyketides and non-ribosomal peptides, produced by the pathogen play important roles in disease and development. However, there is currently no knowledge on the composition or biological activity of the volatile organic compounds (VOCs) secreted by P. nodorum. To address this, we undertook a series of growth and phytotoxicity assays and demonstrated that P. nodorum VOCs inhibited bacterial growth, were phytotoxic and suppressed self-growth. Mass spectrometry analysis revealed that 3-methyl-1-butanol, 2-methyl-1-butanol, 2-methyl-1-propanol and 2-phenylethanol were dominant in the VOC mixture and phenotypic assays using these short chain alcohols confirmed that they were phytotoxic. Further analysis of the VOCs also identified the presence of multiple sesquiterpenes of which four were identified via mass spectrometry and nuclear magnetic resonance as β-elemene, α-cyperone, eudesma-4,11-diene and acora-4,9-diene. Subsequent reverse genetics studies were able to link these molecules to corresponding sesquiterpene synthases in the P. nodorum genome. However, despite extensive testing, these molecules were not involved in either of the growth inhibition or phytotoxicity phenotypes previously observed. Plant assays using mutants of the pathogen lacking the synthetic genes revealed that the identified sesquiterpenes were not required for disease formation on wheat leaves. Collectively, these data have significantly extended our knowledge of the VOCs in fungi and provided the basis for further dissecting the roles of sesquiterpenes in plant disease.

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“…TB47, and TB67 by Romoli et al [98]. The solid phase micro extraction gas-chromatography mass-spectrometry technique revealed that some of the produced antimicrobial compounds were very likely volatile organic compounds (VOCs), which are a well-known regulatory factor in the interactions among different organisms in microbial ecosystems, and that their synthesis was constitutive, as it was not induced by the presence of target strains [99]. More interestingly, VOCs seemed to be more effective in inhibiting the growth of Bcc bacteria than most of the commonly used antibiotics.…”

Section: Antimicrobialsmentioning

confidence: 99%

Bacteria Associated with Marine Benthic Invertebrates from Polar Environments: Unexplored Frontiers for Biodiscovery?

Giudice

Rizzo

2018

Diversity

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The ecological function of bacteria-invertebrate interactions in Polar areas remains poorly understood, despite increasing evidence that microbial metabolites may play pivotal roles in host-associated chemical defense and in shaping the symbiotic community structure. The metabolic and physiological changes that these organisms undergo in response to adapting to extreme conditions result in the production of structurally and functionally novel biologically active molecules. Deepening our knowledge on the interactions between bacteria and their invertebrate host would be highly helpful in providing the rationale for why (e.g., competition or cooperative purpose) and which (whether secondary metabolites, enzymes, or proteins) bioactive compounds are produced. To date, cold-adapted bacteria associated with marine invertebrates from the Arctic and Antarctica have not been given the attention they deserve and the versatility of their natural products remains virtually unexplored, even if they could represent a new attractive frontier in the search for novel natural compounds. This review is aimed at showcasing the diversity of cold-adapted bacteria associated with benthic invertebrates from Polar marine areas, highlighting the yet unexplored treasure they represent for biodiscovery.

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GC–MS volatolomic approach to study the antimicrobial activity of the antarctic bacterium Pseudoalteromonas sp. TB41

Cited by 47 publications

References 20 publications

Volatile affairs in microbial interactions

Volatile affairs in microbial interactions

Volatile molecules secreted by the wheat pathogenParastagonospora nodorumare involved in development and phytotoxicity

Bacteria Associated with Marine Benthic Invertebrates from Polar Environments: Unexplored Frontiers for Biodiscovery?

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