GC‐MS structural characterization of fatty acids from marine aerobic anoxygenic phototrophic bacteria

Rontani, Jean‐François; Christodoulou, Stéphane; Koblížek, Michal

doi:10.1007/s11745-005-1364-6

Cited by 36 publications

(37 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples were shipped in a frozen state and analyzed in the Třeboň laboratory, typically within 1 mo after sample collection. As tested with Roseobacter-like strain COL2P (Rontani et al 2005), the freeze-thaw cycle of formaldehyde-fixed samples does not significantly reduce their IR emission. Chlorophyll a (chl a) content was determined by high performance liquid chromatography as described previously or spectrophotometrically in 90% acetone extracts.…”

Section: Methodsmentioning

confidence: 91%

“…The initial claim that the AAP community is dominated by Erythrobacter sp. (Kolber et al 2001) was later dismissed by both culture-free (Béjà et al 2002) and cultivation studies (Rontani et al 2005, Koblí=ek et al 2006. Rontani et al (2005) isolated 3 strains of Erythrobacter and Roseobacter species from the Baltic Sea, which confirms the presence of these genera in the environment.…”

Section: Discussionmentioning

confidence: 99%

“…(Kolber et al 2001) was later dismissed by both culture-free (Béjà et al 2002) and cultivation studies (Rontani et al 2005, Koblí=ek et al 2006. Rontani et al (2005) isolated 3 strains of Erythrobacter and Roseobacter species from the Baltic Sea, which confirms the presence of these genera in the environment. Cottrell et al (2006) attempted to estimate AAP community composition in the MidAtlantic Bight by comparing cell numbers of AAPs with abundances of individual bacterial groups (Erythrobacter, Roseobacter) determined by FISH.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea

Mašı́n¹,

Zdun²,

Stoń-Egiert³

et al. 2006

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

The community of aerobic anoxygenic phototrophs was investigated in the Baltic Sea using infrared epifluorescence microscopy from September 2004 to October 2005. The majority of these bacteriochlorophyll-containing organisms exhibited a specific sickle-shaped morphology, with rods or other morphotypes observed only occasionally. Aerobic anoxygenic phototrophs were observed mostly from April to September (1 to 12% of total prokaryotes), peaking in May 2005 at a concentration of up to 0.38 × 10 6 cells ml -1. This peak was associated with the later phase of the spring bloom. In the later months, the amount of phototrophic bacteria gradually declined until the beginning of the fall mixing, and remained low from November to March, contributing only 0 to 2% of total prokaryotes. A novel technique combining fluorescent in situ hybridization (FISH) and infrared epifluorescence microscopy indicated that the Baltic aerobic anoxygenic phototrophs were mostly Gammaproteobacteria, with a smaller fraction of Alphaproteobacteria. KEY WORDS: Aerobic photosynthetic bacteria · Bacteriochlorophyll a · Photoheterotrophy · Epifluorescence microscopy Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 45: [247][248][249][250][251][252][253][254] 2006 thermocline structure gradually dissolves due to the decrease in irradiance and intensified wind mixing. The autumn bloom (September-October) is dominated by cyanobacteria along with dinophytes and chlorophytes as other main contributors (Sto? et al. 2002, Wasmund & Uhlig 2003. By the end of the fall season, the water column becomes homogenous down to the halocline, with mean water temperatures of 3 to 4°C.Earlier studies suggested that Baltic primary production is mostly limited by nitrogen availability, but the activity of nitrogen-fixing cyanobacteria in the summer period may cause limitation by phosphorus (Kivi et al. 1993, Nausch et al. 2004. Knowledge of Baltic bacterioplankton dynamics pattern is fragmentary. In the coastal zone offshore of Sweden, bacterial cell numbers display a relatively simple pattern, with a minimum in winter and a maximum in summer (Hagström et al. 1979). In early spring, the bacterial community is predominantly controlled by nitrogen availability and nanoflagellate grazing (Kuupo et al. 1998). In some studies, a stimulation of bacterial growth by phosphorus was observed in late spring, whereas in summer a great stimulation was induced by the combined addition of nitrogen and phosphorus (Kivi et al. 1993).In a previous study, we surveyed the presence of AAPs in the Baltic Sea in late summer (August -September) 2003 by IR kinetic fluorometry; at that time, Bchl a concentration varied between 8 and 50 ng l -1 . Interestingly, during our next survey in April 2004, the activity of AAPs was below the detection limit of the instrument (~2 ng Bchl a l -1 ; M. Koblí=ek unpubl. data). This result suggested that the community of AAPs in the Baltic Sea undergoes seasonal changes. For this reason, we followed ...

show abstract

Section: Methodsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea

Mašı́n¹,

Zdun²,

Stoń-Egiert³

et al. 2006

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

show abstract

“…strain NAP1, and Citromicrobium sp. strain CV44, were described previously (29)(30)(31)(32). Freshwater betaproteobacterium strain VA01 was isolated from Velka Amerika lake, Czech Republic, by the dilutionto-extinction technique (M. Mašín, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Distribution and Origin of Oxygen-Dependent and Oxygen-Independent Forms of Mg-Protoporphyrin Monomethylester Cyclase among Phototrophic Proteobacteria

Boldareva-Nuianzina

Bláhová

Sobotka

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

b Magnesium-protoporphyrin IX monomethylester cyclase is one of the key enzymes of the bacteriochlorophyll biosynthesis pathway. There exist two fundamentally different forms of this enzyme. The oxygen-dependent form, encoded by the gene acsF, catalyzes the formation of the bacteriochlorophyll fifth ring using oxygen, whereas the oxygen-independent form encoded by the gene bchE utilizes an oxygen atom extracted from water. The presence of acsF and bchE genes was surveyed in various phototrophic Proteobacteria using the available genomic data and newly designed degenerated primers. It was found that while the majority of purple nonsulfur bacteria contained both forms of the cyclase, the purple sulfur bacteria contained only the oxygenindependent form. All tested species of aerobic anoxygenic phototrophs contained acsF genes, but some of them also retained the bchE gene. In contrast to bchE phylogeny, the acsF phylogeny was in good agreement with 16S inferred phylogeny. Moreover, the survey of the genome data documented that the acsF gene occupies a conserved position inside the photosynthesis gene cluster, whereas the bchE location in the genome varied largely between the species. This suggests that the oxygen-dependent cyclase was recruited by purple phototrophic bacteria very early during their evolution. The primary sequence and immunochemical similarity with its cyanobacterial counterparts suggests that acsF may have been acquired by Proteobacteria via horizontal gene transfer from cyanobacteria. The acquisition of the gene allowed purple nonsulfur phototrophic bacteria to proliferate in the mildly oxygenated conditions of the Proterozoic era.

show abstract

“…The distinctive fatty acids were also explored among different species (Koblížek et al, 2003;Yoon et al, 2004;Rontani et al, 2005). However, no comparison in phospholipid fatty acids (PLFA) has been made between BChl a-containing AAPB and its phylogenetically related bacteria lacking of BChl a.…”

Section: Introductionmentioning

confidence: 99%

Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea

Yang

et al. 2008

Front. Earth Sci. China

View full text Add to dashboard Cite

The comparison of the fatty acids between aerobic anoxygenic phototrophic bacteria (AAPB) and their phylogenetic relatives has been a fascinating but yet enigmatic topic, enhancing our understanding of physiological variations between these evolutionarily related microorganisms. Two strains of marine bacteria, both phylogenetically falling into Erythrobacter sp., were isolated from the South China Sea, and demonstrated, respectively, to be an aerobic anoxygenic phototrophic bacteria (AAPB) (JL475) which is capable of anoxygenic photosynthesis via BChl a, and an obligate heterotroph (JL316) with a lack of BChl a, on the basis of phylogenetic analysis and pure culture cultivation. Phospholipid fatty acids (PLFA) and glycolipid fatty acids (GLFA) of the two strains were extracted and analyzed by gas chromatographymass spectrometry. The PLFA in JL475 AAPB are characterized by C 18∶1 , C 18∶2ω7,13 and C 18∶0 , with the C 18∶2ω7,13 being a specific compound for AAPB and in particular for Erythrobacter longus and some of its phylogenetically closely related relatives. The JL316 strain is characterized in PLFA by the presence of C 18∶1 , C 16∶1 and C 16∶0 , and in particular C 17∶1 . GLFA do not show any discrimination between the two strains. Four α,ω-dicarboxylic acids, including 1,8-octanedioic acid, 1,9-nonanedioic acid, 1,10-decanedioic acid and 1,11-undecanedioic acid, are present only in JL316 GLFA, presumably derived from metabolic products. C 14 -C 16 2-hydroxy fatty acids were found in the two strains, probably assuming a similar function of their LPS in outer membranes.

show abstract

GC‐MS structural characterization of fatty acids from marine aerobic anoxygenic phototrophic bacteria

Cited by 36 publications

References 54 publications

Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea

Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea

Distribution and Origin of Oxygen-Dependent and Oxygen-Independent Forms of Mg-Protoporphyrin Monomethylester Cyclase among Phototrophic Proteobacteria

Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea

Contact Info

Product

Resources

About